17-700 | Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

PCR Analysis of RIP (Fold Enrichment):
RIP was performed using HeLa cell lysate and either anti-SNRNP70 or Normal Rabbit IgG as the immunoprecipitating antibody. Purified RNA was then analyzed by qRT-PCR using RIP Primers specific for the U1 snRNA.
PCR Analysis of RIP (Fold Enrichment):
RIP was performed using HeLa cell lysate and either anti
17-700
12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
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      Overview

      Replacement Information

      Tableau de caractéristiques principal

      Principales applications
      Immunoprécipitation de protéines fixant l'ARN (RIP)
      Description
      Référence 17-700
      Famille de marques Upstate
      Synonymes Magnetic RIP kit
      Nom de marque
      • Upstate
      • Magna RIP
      Description Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
      Aperçu RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

      Features & Benefits:
      -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
      -RNAse inhibitors and RNAse-free reagents
      -Negative controls
      Informations générales Gene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
      products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
      Références bibliographiques
      Informations produit
      Composants
      • Precipitate Enhancer
      • Normal Mouse IgG
      • Rabbit IgG Purified
      • Protease Inhibitor Cocktail 200X
      • RNase Inhibitor
      • Proteinase K (10 mg/mL)
      • Nuclease free water
      • Magnetic Beads Protein A/G
      • RIP Wash Buffer
      • RIP Lysis Buffer
      • 0.5 M EDTA
      • 10% SDS
      • Salt Solution I
      • Salt Solution II
      Présentation Two boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
      Applications
      Application RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.
      Principales applications
      • Immunoprécipitation de protéines fixant l'ARN (RIP)
      Informations biologiques
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
      Informations sur l'emballage
      Quantité 12 assays
      Conditionnement RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      FDS

      Langues
      English

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      MicroRNA-466l Upregulates IL-10 Expression in TLR-Triggered Macrophages by Antagonizing RNA-Binding Protein Tristetraprolin-Mediated IL-10 mRNA Degradation.
      Ma, Feng, et al.
      Journal of immunology (Baltimore, Md. : 1950), (2010) 2010

      Afficher le résumé
      20410487
      Advances in RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray profiling.
      Baroni, Timothy E, et al.
      Methods Mol. Biol., 419: 93-108 (2008) 2008

      Afficher le résumé
      18369977
      Ribonomic and short hairpin RNA gene silencing methods to explore functional gene programs associated with tumor growth arrest.
      Baroni, Timothy E, et al.
      Methods Mol. Biol., 383: 227-44 (2007) 2007

      Afficher le résumé
      18217689
      RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts.
      Keene, Jack D, et al.
      Nat Protoc, 1: 302-7 (2006) 2006

      Afficher le résumé
      17406249
      Gene expression analysis of messenger RNP complexes.
      Penalva, Luiz O F, et al.
      Methods Mol. Biol., 257: 125-34 (2004) 2004

      Afficher le résumé
      14770002
      Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays.
      Tenenbaum, Scott A, et al.
      Methods, 26: 191-8 (2002) 2002

      Afficher le résumé
      12054896
      Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays.
      Tenenbaum, S A, et al.
      Proc. Natl. Acad. Sci. U.S.A., 97: 14085-90 (2000) 2000

      Afficher le résumé
      11121017
      Soluble and particulate metals in the Po River: factors affecting concentrations and partitioning.
      M Pettine,M Camusso,W Martinotti,R Marchetti,R Passino,G Queirazza
      The Science of the total environment 145 1994

      Afficher le résumé
      8023132

      Brochure

      Titre
      RNA-Binding Protein Immunoprecipitation

      Manuels d'utilisation

      Titre
      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

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      Catégories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits