07-352 | Anti-acetyl-Histone H3 (Lys9) Antibody

Beadlyte® Histone-Peptide Specificity Assay
Representative data from a previous lot.
1:1000-1:5000 dilutions were incubated with a cocktail of microspheres conjugated to Histone H3 peptides with the following modifications:

1. acetyl-lysine 9
2. acetyl-
Beadlyte® Histone-Peptide Specificity Assay
Representative data from a previous lot.
1:10
07-352
100 µL  
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      Overview

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      Tableau de caractéristiques principal

      Species Reactivity Key Applications Host Format Antibody Type
      Yeast (S. cerevisiae),H,Vrt ChIP,WB,Mplex,ChIP-seq Rb Serum Polyclonal Antibody
      Description
      Catalogue Number
      Brand Family Upstate
      Synonyms
      Trade Name
      • Upstate
      Description Anti-acetyl-Histone H3 (Lys9) Antibody
      Background Information Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      Format Serum
      Specificity Recognizes acetyl-histone H3 (Lys9), Mr 17 kDa. An additional unknown protein was detected at Mr 23 kDa.
      Control
      • TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
      Presentation Antiserum containing 0.35% sodium azide and 30% glycerol.
      Applications
      Application Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H3 (Lys9) also known as H3K9Ac, Histone H3 (acetyl K9) & has been validated in ChIP, WB, Mplex.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • Multiplexing
      • ChIP-seq
      Application Notes Chromatin Immunoprecipitation (ChIP):
      An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.

      Beadlyte ® Histone-Peptide Specificity Assay:
      A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No cross-reactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.
      Biological Information
      Immunogen Ovalbumin-conjugated, synthetic peptide (KQTARAcKSTGGK-C) corresponding to amino acids 4-14 of yeast histone H3 acetylated on lysine 9, with a C-terminal cysteine added for conjugation purposes.
      Epitope a.a. 4-14
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rabbit
      Specificity Recognizes acetyl-histone H3 (Lys9), Mr 17 kDa. An additional unknown protein was detected at Mr 23 kDa.
      Isotype
      Species Reactivity
      Species Reactivity Note Yeast and human. Broad species cross-reactivity is expected.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • H3/t
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      Modifications
      • Acetylation
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by western blot on acid extracts from sodium butyrate treated HeLa cells.

      Western Blot Analysis:
      A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells (Catalog # 17-305).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size 100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      FDS

      Langues
      Deutschland

      Certificats d'analyse

      TitreNuméro de lot
      Anti-acetyl-Histone H3 (Lys9) 2465196
      Anti-acetyl-Histone H3 (Lys9) - 2388934 2388934
      Anti-acetyl-Histone H3 (Lys9) - 2090152 2090152
      Anti-acetyl-Histone H3 (Lys9) - 2203126 2203126
      Anti-acetyl-Histone H3 (Lys9) - 22399 22399
      Anti-acetyl-Histone H3 (Lys9) - 2325091 2325091
      Anti-acetyl-Histone H3 (Lys9) - 24661 24661
      Anti-acetyl-Histone H3 (Lys9) - 28741 28741
      Anti-acetyl-Histone H3 (Lys9) - 31388 31388
      Anti-acetyl-Histone H3 (Lys9) - DAM1576933 DAM1576933
      Anti-acetyl-Histone H3 (Lys9) - DAM1658697 DAM1658697
      Anti-acetyl-Histone H3 (Lys9) - DAM1724049 DAM1724049
      Anti-acetyl-Histone H3 (Lys9) - DAM1767487 DAM1767487
      Anti-acetyl-Histone H3 (Lys9) - DAM1813175 DAM1813175

      Références bibliographiques | 49 Disponible | Voir toutes les références

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      Plant physiology and biochemistry : PPB / Société française de physiologie végétale 57 2012

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      RORγ directly regulates the circadian expression of clock genes and downstream targets in vivo.
      Yukimasa Takeda,Raja Jothi,Veronique Birault,Anton M Jetten
      Nucleic acids research 40 2012

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      Promoter-associated small double-stranded RNA interacts with heterogeneous nuclear ribonucleoprotein A2/B1 to induce transcriptional activation.
      Jia Hu,Zhong Chen,Ding Xia,Jia Wu,Hua Xu,Zhang-Qun Ye
      The Biochemical journal 447 2012

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      23035981
      5-azacitidine in aggressive myelodysplastic syndromes regulates chromatin structure at PU.1 gene and cell differentiation capacity.
      N Curik,P Burda,K Vargova,V Pospisil,M Belickova,P Vlckova,F Savvulidi,E Necas,H Hajkova,C Haskovec,J Cermak,M Krivjanska,M Trneny,P Laslo,A Jonasova,T Stopka
      Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 26 2012

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      The transcription factor Pax5 regulates its target genes by recruiting chromatin-modifying proteins in committed B cells.
      McManus, S; Ebert, A; Salvagiotto, G; Medvedovic, J; Sun, Q; Tamir, I; Jaritz, M; Tagoh, H; Busslinger, M
      The EMBO journal 30 2388-404 2011

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      Sgf29 binds histone H3K4me23 and is required for SAGA complex recruitment and histone H3 acetylation.
      Bian C, Xu C, Ruan J, Lee KK, Burke TL, Tempel W, Barsyte D, Li J, Wu M, Zhou BO, Fleharty BE, Paulson A, Allali-Hassani A, Zhou JQ, Mer G, Grant PA, Workman JL, Zang J, Min J.
      The EMBO journal 30 2829-42 2011

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      Trichostatin a modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viability.
      Gómez MC, Pope CE, Biancardi MN, Dumas C, Galiguis J, Morris AC, Wang G, Dresser BL
      Cellular reprogramming 13 315-29. doi 2011

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      Brain-specific Expression of N-Acetylglucosaminyltransferase IX (GnT- IX) Is Regulated by Epigenetic Histone Modifications.
      Kizuka Y, Kitazume S, Yoshida M, Taniguchi N
      The Journal of biological chemistry 286 31875-84. Epub 2011 Jul 19. 2011

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      Additional sex comb-like (ASXL) proteins 1 and 2 play opposite roles in adipogenesis via reciprocal regulation of peroxisome proliferator-activated receptor {gamma}.
      Park UH, Yoon SK, Park T, Kim EJ, Um SJ
      J Biol Chem 286 1354-63. Epub 2010 Nov 3. 2011

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      HBO1 is required for H3K14 acetylation and normal transcriptional activity during embryonic development.
      Kueh AJ, Dixon MP, Voss AK, Thomas T
      Mol Cell Biol 31 845-60. Epub 2010 Dec 13. 2011

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