07-1334 | Anti-JunD Antibody

Immunocytochemistry Analysis: Confocal fluorescent analysis of TPA-treated HeLa cells using anti-JunD (Red). Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue).
Immunocytochemistry Analysis: Confocal fluorescent analysis of TPA-treated HeLa cells using anti-Ju
07-1334
100 µg  
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      Overview

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      Tableau de caractéristiques principal

      Espèces réactivesPrincipales applicationsHôteFormatType d'anticorps
      Bovins, Humain, Souris, RWB, ImmunocytochimieLapinPurifié(e) par affinitéAnticorps polyclonal
      Description
      Référence 07-1334
      Synonymes transcription factor jun-D JunD-FL isoform activator protein 1 jun D proto-oncogene
      Description Anti-JunD Antibody
      Informations générales JunD is a member of the JUN family, and a functional component of the AP1 transcription factor complex. JunD binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site. It also binds DNA as a dimer. Furthermore, JunD has been proposed to protect cells from p53-dependent senescence and apoptosis.
      Références bibliographiques
      Informations produit
      Format Purifié(e) par affinité
      Spécificité This antibody recognizes JunD.
      Contrôle
      • HeLa cell lysate
      Présentation Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
      Applications
      Application Anti-JunD Antibody is a Rabbit Polyclonal Antibody for detection of JunD also known as JunD-FL isoform, transcription factor jun-D & has been validated in ICC & WB.
      Principales applications
      • Western Blotting
      • Immunocytochimie
      Notes opératoires Western Blot (SNAP ID Technique):
      A 1:2,000 dilution from a previous lot detected JunD using the SNAP ID analysis.
      Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected JunB in TPA-treated HeLa lysates.
      Immunocytochemistry Analysis:
      Representative images from a previous lot.
      Informations biologiques
      Espèces d'immunogène KLH-conjugated linear peptide corresponding to human JunD.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Lapin
      Spécificité This antibody recognizes JunD.
      Espèces réactives Bovins Humain Souris Rat
      Species Reactivity Note Proven to react with human.
      Predicted to react with mouse, rat, and cow based on 100% sequence homology.
      Type d'anticorps Anticorps polyclonal
      Code Entrez Gene
      Résumé Entrez Gene The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms.
      Symbole du gène
      • AP-1
      Méthode de purification Affinity Purfied
      Code UniProt
      Résumé UniProt FUNCTION: Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.
      SUBUNIT STRUCTURE: Binds DNA as a dimer By similarity.
      SUBCELLULAR LOCATION: Nucleus.
      SEQUENCE SIMILARITIES: Belongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP domain.
      SEQUENCE CAUTION: The sequence CAA40010.1 differs from that shown. Reason: Frameshift at several positions.
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Evaluated by Western Blot in TPA-treated HeLa cell lysate.
      Western Blot Analysis: 0.25 µg/mL of this antibody detected JunD in 10 µg of TPA-treated HeLa cell lysate. Antibody will also work in untreated HeLa cells and lysate.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable at 2-8°C for 1 year from date of receipt.
      Informations sur l'emballage
      Taille du matériau 100 µg
      Informations sur le transport
      Information complémentaire
      Caractéristiques