06-942 | Anti-acetyl-Histone H3 (Lys9) Antibody

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10e5 cell equivalents per IP) were subjected to chromatin immunoprecipitation with the Magna ChIP™ HiSense Kit (Cat. # 17-10460), using 5 μg of either a negative control rabbit IgG or Cat. No 06-942, Anti-Acetyl-Histone H3 (Lys9) antibody. Successful immunoprecipitation of acetyl-Histone H3 (Lys9)-associated DNA fragments was verified by qPCR using ChIP Primers GAPDH promoter (Part No. 22-004) as a positive locus, and β-Globin primers as a negative locus.
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from
200 µg  
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      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      R, Humain, SourisChIP-seq, WB, ChIP, DB, Cytométrie en fluxLapinPurifié(e) Anticorps polyclonal
      Référence 06-942
      Replaces 04-1003
      Famille de marques Upstate
      Nom de marque
      • Upstate
      Description Anti-acetyl-Histone H3 (Lys9) Antibody
      Alternate Names
      • histone cluster 3, H3
      • H3K9Ac
      • Histone H3 (acetyl K9)
      • H3 histone family, member T
      • histone 3, H3
      • histone 3
      • H3
      Informations générales Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Références bibliographiques
      Informations produit
      Format Purifié(e)
      Présentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
      Application Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot blot (DB), published in peer reviewed journals, and validated in WB, ChIP, ChIP-seq, FC.
      Principales applications
      • ChIP-seq
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Dot Blot
      • Cytométrie en flux
      Notes opératoires Chromatin Immunoprecipitation/ChIP-seq Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys9) in 5 µg of HeLa cell lysate.
      Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.
      Informations biologiques
      Espèces d'immunogène KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.
      Épitope Surrounding H3K9ac
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Lapin
      Spécificité Histone H3 acetylated at position 9.
      Isotype IgG
      Espèces réactives Rat Humain Souris
      Species Reactivity Note Human, Mouse, Rat, although this peptide sequence is identical in a wide range of animal and plant species.
      Type d'anticorps Anticorps polyclonal
      Code Entrez Gene
      Symbole du gène
      • MGC126886
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • Acétylation
      Méthode de purification Protein A purfied
      Code UniProt
      Informations physico-chimiques
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Informations sur l'emballage
      Quantité 200 µg
      Informations sur le transport
      Information complémentaire




      Certificats d'analyse

      TitreNuméro de lot
      Anti-acetyl-Histone H3 (Lys9) - 21351722135172
      Anti-acetyl-Histone H3 (Lys9) - 1874018740
      Anti-acetyl-Histone H3 (Lys9) - 19734141973414
      Anti-acetyl-Histone H3 (Lys9) - 21112832111283
      Anti-acetyl-Histone H3 (Lys9) - 21784192178419
      Anti-acetyl-Histone H3 (Lys9) - 2189721897
      Anti-acetyl-Histone H3 (Lys9) - 21931542193154
      Anti-acetyl-Histone H3 (Lys9) - 22795102279510
      Anti-acetyl-Histone H3 (Lys9) - 2399723997
      Anti-acetyl-Histone H3 (Lys9) - 25110872511087
      Anti-acetyl-Histone H3 (Lys9) - 25621002562100
      Anti-acetyl-Histone H3 (Lys9) - 3163631636
      Anti-acetyl-Histone H3 (Lys9) -25246852524685
      Anti-acetyl-Histone H3 (Lys9) -25493942549394
      Anti-acetyl-Histone H3 (Lys9), - 23878122387812
      Anti-acetyl-Histone H3 (Lys9), - 24287142428714

      Références bibliographiques | 29 Disponible | Voir toutes les références

      Aperçu de la référence bibliographiqueApplicationNº PubMed
      Association of the interferon-β gene with pericentromeric heterochromatin is dynamically regulated during virus infection through a YY1-dependent mechanism.
      T Josse,H Mokrani-Benhelli,R Benferhat,E Shestakova,Z Mansuroglu,H Kakanakou,A Billecocq,M Bouloy,Eliette Bonnefoy
      Nucleic acids research 40 2012

      Afficher le résumé
      An essential role for a mammalian SWI/SNF chromatin-remodeling complex during male meiosis.
      Yuna Kim,Andrew M Fedoriw,Terry Magnuson
      Development (Cambridge, England) 139 2012

      Afficher le résumé
      Epigenetic reprogramming as a key contributor to melanocyte malignant transformation.
      Molognoni F, Cruz AT, Meliso FM, Morais AS, Souza CF, Xander P, Bischof JM, Costa FF, Soares MB, Liang G, Jones PA, Jasiulionis MG.
      Epigenetics : official journal of the DNA Methylation Society 6 450-64 2011

      Afficher le résumé
      Mouse Rankl expression is regulated in T cells by c-Fos through a cluster of distal regulatory enhancers designated the T cell control region.
      Bishop KA, Coy HM, Nerenz RD, Meyer MB, Pike JW
      The Journal of biological chemistry 2011

      Afficher le résumé
      Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation.
      Weake, Vikki M, et al.
      Genes Dev., 25: 1499-509 (2011) 2011

      Afficher le résumé
      SIRT6 in mouse spermatogenesis is modulated by diet-induced obesity.
      Palmer NO, Fullston T, Mitchell M, Setchell BP, Lane M.
      Reproduction, fertility, and development 23 929-39 2011

      Afficher le résumé
      MDM2 recruitment of lysine methyltransferases regulates p53 transcriptional output.
      Chen L, Li Z, Zwolinska AK, Smith MA, Cross B, Koomen J, Yuan ZM, Jenuwein T, Marine JC, Wright KL, Chen J
      EMBO J 2010

      Afficher le résumé
      Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells.
      S Barrand, P Collas
      Biochemical and biophysical research communications 391 762-7 2010

      Afficher le résumé
      Polycomb target genes are silenced in multiple myeloma.
      Kalushkova A, Fryknäs M, Lemaire M, Fristedt C, Agarwal P, Eriksson M, Deleu S, Atadja P, Osterborg A, Nilsson K, Vanderkerken K, Oberg F, Jernberg-Wiklund H
      PLoS One 5 e11483. 2010

      Afficher le résumé Article en texte intégral
      Chromatin environment of histone variant H3.3 revealed by quantitative imaging and genome-scale chromatin and DNA immunoprecipitation.
      Delbarre E, Jacobsen BM, Reiner AH, Sørensen AL, Küntziger T, Collas P
      Mol Biol Cell 21 1872-84. Epub 2010 Apr 7. 2010

      Afficher le résumé Article en texte intégral

      Informations techniques

      Anti-acetyl-Histone H3 (Lys9) ChIP-Seq Analysis