06-1047 | Anti-IRF4 Antibody, NT

Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of PC3 cells using Anti-IRF4. Actin filaments have been labeled with Alexa Fluor 488-Phalloidin (Green), and nuclei are stained with DAPI (Blue) (left image). Anti-IRF4 shows positive cytosolic and nuclear staining (Red) (right image).
Immunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of PC3 cells u
06-1047
100 µg  
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      Espèces réactives Principales applications Hôte Format Type d'anticorps
      R, Humain, Souris, ChevalImmunocytochimie, WBLapinPurifié(e) par affinitéAnticorps polyclonal
      Description
      Référence 06-1047
      Description Anti-IRF4 Antibody, NT
      Alternate Names
      • interferon regulatory factor 4
      • Lymphocyte-specific interferon regulatory factor
      • Multiple myeloma oncogene
      Informations générales Interferon regulatory factor 4, also known as IRF4, belongs to the IRF family. IRF4 encodes a 450 amino acid polypeptide with a predicted molecular weight of 51.6 kDa (Grossman 1996). The IRF4 gene is expressed in the spleen, lymphocytes and melanocytes, and is induced in to T cells after cross linking with the T cell receptor. IRF4 is a transcriptional activator that binds to the interferon stimulated response element (ISRE) of the MHC class I promoter. IRF4 is essential for mature T and B lymphocyte function. IRF4 interacts with MYD88 to prevent the interaction of IRF5, resulting in negative regulation of Toll Like Receptor (TLR) signaling. High levels of IRF4 have been associated with Epstein Barr Virus (EBV) and may be critical to EBV transformation. Further oncogenic potential has been associated with the involvement of IRF4 with hair color and pigmentation of the skin, which may cause multiple myeloma.
      Références bibliographiques
      Informations produit
      Format Purifié(e) par affinité
      Contrôle
      • Mouse brain lysate.
      Présentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application Anti-IRF4 Antibody, N-terminus detects level of IRF4 & has been published & validated for use in WB & IC.
      Principales applications
      • Immunocytochimie
      • Western Blotting
      Notes opératoires Immunocytochemistry: 1:500 dilution of a previous lot of this antibody was used on immunocytochemistry in PC3 cells.
      Informations biologiques
      Espèces d'immunogène KLH conjugated linear peptide corresponding to the N-terminal region of IRF4.
      Épitope N-Terminus
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Lapin
      Spécificité This antibody recognizes the N-terminal region of IRF4 at and around amino acids 177-194.
      Espèces réactives Rat Humain Souris Cheval
      Species Reactivity Note Tested to react with human and mouse. Predicted to react with rat and horse based on sequence homology.
      Type d'anticorps Anticorps polyclonal
      Code Entrez Gene
      Symbole du gène
      • NF-EM5
      • IRF-4
      • LSIRF
      • MUM1
      Méthode de purification Affinity Purfied
      Code UniProt
      Résumé UniProt Function: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
      Subunit structure: Interacts with SPIB and DEF6. Ref.5 Ref.6
      Subcellular location: Nucleus.
      Tissue specificity: Lymphoid cells.
      Induction: Not induced by interferons.
      Involvement in disease: A chromosomal aberration involving IRF4 may be a cause of multiple myeloma [MIM:254500]. Translocation t(6;14)(p25;q32) with the IgH locus.
      Sequence similarities: Belongs to the IRF family.
      Contains 1 tryptophan pentad repeat DNA-binding domain.

      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Evaluated by western blot on mouse brain lysate.
      Western Blot Analysis: 0.5 μg/mL of this antibody detected IRF4 in 10 μg of mouse brain lysate.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable for 1 year at 2-8ºC from date of receipt.
      Informations sur l'emballage
      Quantité 100 µg
      Informations sur le transport
      Information complémentaire
      Caractéristiques