05-904 | Anti-HA Tag Antibody

Western Blot Analysis
Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-HA Tag (0.5 μg/mL).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
Western Blot Analysis
Lysates from untransfected (lane 1) and transfected (lane 2) HEK293 cells
05-904
200 µg  
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      Overview

      Replacement Information
      Replacement Information 05-904 is a recommended replacement for 07-221

      Offres spéciales

      Tableau de caractéristiques principal

      Species Reactivity Key Applications Host Format Antibody Type
      Vrt ICC, WB, IP M Purified Monoclonal Antibody
      Description
      Catalogue Number 05-904
      Replaces 07-221
      Brand Family Upstate
      Trade Name
      • Upstate
      Description Anti-HA Tag Antibody
      Overview Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
      Background Information Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
      References
      Product Information
      Format Purified
      Control
      • RIPA lysates from HEK293 cells.
      Presentation Purified mouse monoclonal IgG3 in buffer containing PBS, pH 7.4 containing 0.05% sodium azide.
      Applications
      Application Detect HA Tag using this Anti-HA Tag Antibody validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      Application Notes Immunoprecipitation:
      5 μg/mL of his antibody has been reported by an independent laboratory to immunoprecipitate recombinant HA-tagged fusion protein.

      Immunocytochemistry:
      5 μg/mL of this antibody has been reported by an independent laboratory to immunostain HA-tagged fusion protein.
      Biological Information
      Immunogen KLH-conjugated, synthetic peptide corresponding to amino acids 98-106 of influenza hemaglutinin.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Recognizes HA tagged proteins overexpressed in cells, including both amino or carboxy-termini of targeted proteins in transfected mammalian cells.
      Isotype IgG3
      Species Reactivity Vertebrates
      Species Reactivity Note Most common vertebrate species tested. Reactivity with other species has not been confirmed.
      Antibody Type Monoclonal Antibody
      Purification Method Protein A purfied
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by western blot of recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected a recombinant protein containing the HA-tag at the amino terminus in RIPA lysates from HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size 200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificats d'analyse

      TitreNuméro de lot
      Anti-HA Tag (mouse monoclonal IgG3) - DAM1462563DAM1462563
      Anti-HA Tag - 23911052391105
      Anti-HA Tag - 24525202452520
      Anti-HA Tag - 06060335510606033551
      Anti-HA Tag - 06080384210608038421
      Anti-HA Tag - 06100439940610043994
      Anti-HA Tag - 06110459410611045941
      Anti-HA Tag - 19848371984837
      Anti-HA Tag - 20448182044818
      Anti-HA Tag - 20645162064516
      Anti-HA Tag - 21906332190633
      Anti-HA Tag - 22763342276334
      Anti-HA Tag - 24912162491216
      Anti-HA Tag - 3038430384
      Anti-HA Tag - 3243632436
      Anti-HA Tag - 3335133351
      Anti-HA Tag - DAM1394781DAM1394781
      Anti-HA Tag - DAM1676171DAM1676171
      Anti-HA Tag - DAM1690645DAM1690645
      Anti-HA Tag - DAM1718050DAM1718050
      Anti-HA Tag - DAM1770288DAM1770288
      Anti-HA Tag - DAM1794298DAM1794298
      Anti-HA Tag - JBC1381963JBC1381963
      Anti-HA Tag - JBC1879872JBC1879872
      Anti-HA Tag - JBC1900557JBC1900557
      Anti-HA Tag -25752672575267
      Anti-HA Tag -26053712605371
      Monoclonal Antibody - JBC1903651JBC1903651

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
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      Cancer research 72 2012

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      Neutrophils activate alveolar macrophages by producing caspase-6-mediated cleavage of IL-1 receptor-associated kinase-M.
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      J Immunol 186 403-10. Epub 2010 Nov 22. 2011

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      A region of the nucleosome required for Multiple types of transcriptional silencing in saccharomyces cerevisiae.
      Prescott ET, Safi A, Rusche LN
      Genetics 188 535-48. Epub 2011 May 5. 2011

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      21546544
      TBC1D24, an ARF6-interacting protein, is mutated in familial infantile myoclonic epilepsy.
      Falace, Antonio, et al.
      Am. J. Hum. Genet., 87: 365-70 (2010) 2010

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      20727515
      Involvement of p21 (waf1) in merlin deficient sporadic vestibular schwannomas.
      Wu H, Chen Y, Wang Z, Li W, Li J, Zhang L, Lu Y
      Neuroscience 2010

      Afficher le résumé
      20600642
      Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
      Terpe, K
      Appl Microbiol Biotechnol, 60:523-33 (2003) 2003

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