05-636 | Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

ICC: Jurkat cells were treated with etoposide and stained with Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (green, right panel; DNA stained with DAPI, left panel).
ICC: Jurkat cells were treated with etoposide and stained with Anti-phospho-Histone H2A.X (Ser139),
05-636
200 µg  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
Minimum Quantity needs to be mulitiple of
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
Demander le prix
Disponibilité limitée Disponibilité limitée
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
      Nous vous tiendrons informé

      Offres spéciales

       

      Contacter le Service Clients

         ComparerClick To Print This Page

      Overview

      Replacement Information

      Offres spéciales

      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      VertébrésChIP, Immunocytochimie, IF, WBSourisPurifié(e) Anticorps monoclonal
      Description
      Référence 05-636
      Replaces MABE205
      Famille de marques Upstate
      Synonymes Histone H2A.X (phospho S139) H2AXS139P
      Nom de marque
      • Upstate
      Description Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
      Informations générales Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      Références bibliographiques
      Informations produit
      Format Purifié(e)
      Contrôle
      • UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue
      Présentation Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
      Applications
      Application Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
      Principales applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunocytochimie
      • Immunofluorescence
      • Western Blotting
      Notes opératoires Additional Referenced Applications:
      Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
      Informations biologiques
      Espèces d'immunogène peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X
      Clone JBW301
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Souris
      Spécificité Recognizes Histone H2A.X phosphorylated at Ser139.
      Isotype IgG1
      Espèces réactives Vertébrés
      Type d'anticorps Anticorps monoclonal
      Code Entrez Gene
      Résumé Entrez Gene Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Symbole du gène
      • H2A.X
      • H2AFX
      • H2AX
      • H2a/x
      • H2A/X
      Modifications
      • Phosphorylation
      Méthode de purification Protein G purfied
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
      SIZE: 143 amino acids; 15145 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      SUBCELLULAR LOCATION: Nucleus.DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
      DOMAIN: SwissProt: P16104 The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
      SIMILARITY: Belongs to the histone H2A family.
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
      Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
      Informations sur l'emballage
      Quantité 200 µg
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      FDS

      Langues
      Argentina Australia Belgie
      Belgien Belgique Brasil
      Ceská Rep Chile China
      Colombia Cyprus Danmark
      Deutschland Eesti Ellas
      España France Hrvatska 
      Iceland India Indonesia
      Italia Korea Latvija
      Lietuva Magyarország Malta
      México Nederland  Norge
      Peru Polska Portugal
      Schweiz Slovenija Slovensko
      South Africa Srpski Standard EU English
      Suisse Suomi Sverige
      Svizzera Taiwan Thailand
      Türkiye United Kingdom Venezuela
      Vietnamese Éire Österreich
      България Россия

      Certificats d'analyse

      TitreNuméro de lot
      Anti-phospho-Histone H2A.X (Ser139), clone JBW3012476967
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (mouse monoclonal IgG1) - DAM1405597DAM1405597
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (mouse monoclonal IgG1) - DAM1474315DAM1474315
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 21166202116620
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 23905262390526
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 23922652392265
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 24204452420445
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 24247002424700
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 24286602428660
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 24419142441914
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 24556022455602
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 07020524470702052447
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 19977191997719
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 20197322019732
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 20645122064512
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 20681772068177
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 21380162138016
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 22025402202540
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2209222092
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 22110832211083
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 22272562227256
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 22397932239793
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 22505242250524
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 22755862275586
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 22763322276332
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 23103552310355
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2376023760
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 25064832506483
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2535825358
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2750527505
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 3252632526
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - DAM1493341DAM1493341
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - DAM1567248DAM1567248
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - DAM1636071DAM1636071
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - DAM1661039DAM1661039
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - DAM1690636DAM1690636
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - DAM1772418DAM1772418
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - DAM1782241DAM1782241
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - JBC1353261JBC1353261
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - JBC1367868JBC1367868
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - JBC1863420JBC1863420
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - JBC1881392JBC1881392
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - NG1904671NG1904671
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - NG1951659NG1951659
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 -25548982554898
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 -25857932585793

      Références bibliographiques | 99 Disponible | Voir toutes les références

      Aperçu de la référence bibliographiqueNº PubMed
      PARP-1 dependent recruitment of the amyotrophic lateral sclerosis-associated protein FUS/TLS to sites of oxidative DNA damage.
      Rulten, Stuart L, et al.
      Nucleic Acids Res., 42: 307-14 (2014) 2014

      Afficher le résumé
      24049082
      APLF promotes the assembly and activity of non-homologous end joining protein complexes.
      Grundy, Gabrielle J, et al.
      EMBO J., 32: 112-25 (2013) 2013

      Afficher le résumé
      23178593
      Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
      Tang, Jiangbo, et al.
      Nat. Struct. Mol. Biol., 20: 317-25 (2013) 2013

      Afficher le résumé
      23377543
      Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
      Culver-Cochran, Ashley E and Chadwick, Brian P
      BMC Genomics, 14: 740 (2013) 2013

      Afficher le résumé
      24168170
      Homologous recombination DNA repair genes play a critical role in reprogramming to a pluripotent state.
      González, Federico, et al.
      Cell Rep, 3: 651-60 (2013) 2013

      Afficher le résumé
      23478019
      PARP inhibition sensitizes to low dose-rate radiation TMPRSS2-ERG fusion gene-expressing and PTEN-deficient prostate cancer cells.
      Chatterjee, Payel, et al.
      PLoS ONE, 8: e60408 (2013) 2013

      Afficher le résumé
      23565244
      The activation of DNA damage detection and repair responses in cleavage-stage rat embryos by a damaged paternal genome.
      Lisanne Grenier,Bernard Robaire,Barbara F Hales
      Toxicological sciences : an official journal of the Society of Toxicology 127 2012

      Afficher le résumé
      22454429
      Role of SUMO modification of human PCNA at stalled replication fork.
      Himabindu Gali,Szilvia Juhasz,Monika Morocz,Ildiko Hajdu,Karoly Fatyol,Valeria Szukacsov,Peter Burkovics,Lajos Haracska
      Nucleic acids research 40 2012

      Afficher le résumé
      22457066
      The autophagy-senescence connection in chemotherapy: must tumor cells (self) eat before they sleep?
      Rachel W Goehe,Xu Di,Khushboo Sharma,Molly L Bristol,Scott C Henderson,Kristoffer Valerie,Francis Rodier,Albert R Davalos,David A Gewirtz
      The Journal of pharmacology and experimental therapeutics 343 2012

      Afficher le résumé
      22927544
      The 19S proteasome subunit Rpn7 stabilizes DNA damage foci upon genotoxic insult.
      Avgi Tsolou,Glyn Nelson,Varvara Trachana,Niki Chondrogianni,Gabriele Saretzki,Thomas von Zglinicki,Efstathios S Gonos
      IUBMB life 64 2012

      Afficher le résumé
      22473755

      Brochure

      Titre
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

      FAQ

      QuestionRéponse
      How can I check the phosphospecificity of my antibody once my protein is already immobilized on a membrane? You can check for the phosphospecificity of your antibody using Lambda Protein Phosphatase. Simply perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a cell lysate and transfer the proteins to your membrane of choice. Wash the blotted nitrocellulose twice with water. Block the blotted nitrocellulose in freshly prepared TBS containing 1% bovine serum albumin (BSA) and 0.1% Triton X-100 for 1 hour at 20-25°C with constant agitation. Incubate the nitrocellulose in TBS containing 1% bovine serum albumin (BSA), 0.1% Triton X-100 and 2 mM MnCl2, and where dephosphorylation of proteins is desirable, 400 U/ml Lambda Protein Phosphatase for two hours at room temperature, or overnight at 4°C. After incubation, wash the nitrocellulose in PBS-0.1% Tween 20 for 3-5 minutes. Rinse the nitrocellulose in 4-5 changes of water. Continue with your western blotting assay.

      Produits & Applications associés

      Alternative Packsize

       Référence Description  
      05-636-KC Anti-Phos-Hist H2A.1 (S139) (KC) Prix & Disponibilité

      Alternative Format

       Référence Description  
      16-193 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate Prix & Disponibilité
      16-202A Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate Prix & Disponibilité

      Familles de produits

      View Gene Related Products for this Target

      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies