05-1050 | 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

Lysates from various cells with various treatments to stimulated varying tyrosine phosphorylations on multiple substrates were resolved in parallel by SDS-PAGE and transferred to PVDF membrane. The membranes were probed with one of three different anti-phosphotyrosine antibodies, 4G10® (Cat. 05-321), PY20 (Cat. No. 05-947), or 4G10® Platinum (Cat. No. 05-1050). The various blots show the advantages of the anti-phosphotyrosine monoclonal cocktail, 4G10® Platinum.
Lysates from various cells with various treatments to stimulated varying tyrosine phosphorylations o
05-1050
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      Overview

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      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      ToutImmunoprécipitation, WB, Immunocytochimie, ELISA, Immunohistochimie, IF, Cytométrie en fluxSourisPurifié(e) Anticorps monoclonal
      Description
      Référence 05-1050
      Famille de marques Upstate
      Nom de marque
      • Upstate
      Description 4G10® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)
      Informations générales The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of.
      Références bibliographiques
      Informations produit
      Format Purifié(e)
      Spécificité Tyrosine-phosphorylated proteins from all species
      Contrôle
      • Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      Présentation Proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide.
      Liquid at 2-8°C.
      Applications
      Application 4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & IP.
      Principales applications
      • Immunoprécipitation
      • Western Blotting
      • Immunocytochimie
      • ELISA
      • Immunohistochimie
      • Immunofluorescence
      • Cytométrie en flux
      Notes opératoires Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.
      Informations biologiques
      Espèces d'immunogène 4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein
      Hôte Souris
      Spécificité Tyrosine-phosphorylated proteins from all species
      Isotype IgG2b
      Espèces réactives Tout
      Type d'anticorps Anticorps monoclonal
      Modifications
      • Phosphorylation
      Méthode de purification Protein G-Sepharose™ chromatography
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Evaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

      Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable for 1 year at from date of receipt.
      Informations sur l'emballage
      Quantité 100 µL
      Informations sur le transport
      Information complémentaire
      Caractéristiques

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      Certificats d'analyse

      TitreNuméro de lot
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 21160282116028
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 20239212023921
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 19581151958115
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 20065342006534
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 21899382189938
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1535707DAM1535707
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM165529DAM165529
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1713267DAM1713267
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1739309DAM1739309
      4G10#174; Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - DAM1823498DAM1823498
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1462606DAM1462606
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1493437DAM1493437
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1514057DAM1514057
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1548638DAM1548638
      4G10#174; Platinum, Anti-Phosphotyrosine - DAM1774799DAM1774799
      4G10#174; Platinum, Anti-Phosphotyrosine - JBC1870017JBC1870017
      4G10#174; Platinum, Anti-Phosphotyrosine - JBC1917752JBC1917752
      4G10#174; Platinum, Anti-Phosphotyrosine - R0709B0207R0709B0207
      4G10#174; Platinum, Anti-Phosphotyrosine(mouse monoclonal cocktail IgG2b) - DAM1581058DAM1581058
      4G10® Platinum, Anti-Phosphotyrosine2475690
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 23922672392267
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 24304142430414
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 24441212444121
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 22971972297197
      4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) - 23410822341082
      4G10® Platinum, Anti-Phosphotyrosine -25178202517820
      4G10® Platinum, Anti-Phosphotyrosine -25670632567063

      Références bibliographiques | 17 Disponible | Voir toutes les références

      Aperçu de la référence bibliographiqueEspèceNº PubMed
      Wnt proteins regulate acetylcholine receptor clustering in muscle cells.
      Bin Zhang,Chuan Liang,Ryan Bates,Yiming Yin,Wen-Cheng Xiong,Lin Mei
      Molecular brain 5 2012

      Afficher le résumé
      22309736
      The metastasis gene NEDD9 product acts through integrin β3 and Src to promote mesenchymal motility and inhibit amoeboid motility.
      Jessica Ahn,Victoria Sanz-Moreno,Christopher J Marshall
      Journal of cell science 125 2012

      Afficher le résumé
      22328516
      Pro-neural miR-128 is a glioma tumor suppressor that targets mitogenic kinases.
      T Papagiannakopoulos,D Friedmann-Morvinski,P Neveu,J C Dugas,R M Gill,E Huillard,C Liu,H Zong,D H Rowitch,B A Barres,I M Verma,K S Kosik
      Oncogene 31 2012

      Afficher le résumé
      21874051
      Prolactin-induced activation of phagocyte NADPH oxidase in the teleost fish gilthead seabream involves the phosphorylation of p47phox by protein kinase C.
      Víctor H Olavarría,Jaime E Figueroa,Victoriano Mulero
      Developmental and comparative immunology 36 2012

      Afficher le résumé
      21884725
      Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBP? expression and contributes to transformation of breast tumor cells.
      Tapasree Roysarkar,Shikha Sharan,Jun Wang,Snehalata A Pawar,Carrie A Cantwell,Peter F Johnson,Deborah K Morrison,Ju-Ming Wang,Esta Sterneck,Tapasree Roy Sarkar,Tapasree Roy Sarkar
      Molecular and cellular biology 32 2012

      Afficher le résumé
      22037769
      Omega-3 fatty acids modulate collagen signaling in human platelets.
      Larson, M K, et al.
      Prostaglandins Leukot. Essent. Fatty Acids, 84: 93-8 2011

      Afficher le résumé
      21177087
      Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.
      Driessens G, Zheng Y, Locke F, Cannon JL, Gounari F, Gajewski TF
      J Immunol 186 784-90. Epub 2010 Dec 13. 2011

      Afficher le résumé
      21149602
      A PATHOBIOLOGICAL ROLE OF THE INSULIN RECEPTOR IN CHRONIC LYMPHOCYTIC LEUKEMIA.
      Saiya-Cork K, Collins R, Parkin B, Ouillette P, Kuizon E, Kujawski L, Erba H, Campagnaro E, Shedden K, Kaminski M, Malek SN
      Clin Cancer Res 2011

      Afficher le résumé
      21307146
      Participation of the cell polarity protein PALS1 to T-cell receptor-mediated NF-κB activation.
      Carvalho, Gabrielle, et al.
      PLoS ONE, 6: e18159 (2011) 2011

      Afficher le résumé
      21479189
      Osmotic stress stimulates phosphorylation and cellular expression of heat shock proteins in rhesus macaque sperm.
      Cole JA, Meyers SA
      Journal of andrology 32 402-10. Epub 2010 Nov 18. 2011

      Afficher le résumé
      21088232

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      Familles de produits

      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies