70773 | pET Expression System 26b

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      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      70773-3
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          Descripción
          Visión de conjunto pET Expression Systems and pET
          pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

          Components: pET Expression Systems
          Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
          • 10 µg pET vector DNA (for each indicated plasmid)
          • 0.2 ml BL21 Glycerol Stock
          • 0.2 ml BL21(DE3) Glycerol Stock
          • 0.2 ml BL21(DE3)pLysS Glycerol Stock
          • 0.2 ml Induction Control Glycerol Stock

          Components: pET Expression Systems plus Competent Cells
          pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
          • 0.2 ml NovaBlue Competent Cells
          • 0.2 ml BL21(DE3) Competent Cells
          • 0.2 ml BL21(DE3)pLysS Competent Cells
          • 2 × 0.2 ml SOC Medium
          • 10 µl Test Plasmid

          These components are sufficient for up to 10 transformations in each host.

          Purification and Detection Reagents
          Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

          pET Expression System 26b
          The pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB071VM.




          Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          Número de catálogo 70773
          Marca de familia Novagen®
          Referencias bibliográficas
          Información del producto
          Componentes
          Marcador de fusión His•Tag, HSV•Tag
          Aplicaciones
          Información biológica
          Información fisicoquímica
          Dimensiones
          Información de materiales
          Información toxicológica
          Información de seguridad según el GHS
          Información de seguridad
          Declaraciones de uso del producto
          Información de almacenamiento y transporte
          Código de envío Shipped with Blue Ice or with Dry Ice
          Toxicidad Standard Handling
          Almacenamiento ≤ -70°C
          Evitar congelar/descongelar Avoid freeze/thaw
          No congelar No
          Información sobre embalaje
          Información de transporte
          Información complementaria
          Especificaciones

          Documentation

          Ficha datos de seguridad (MSDS)

          Idiomas
          Danmark Deutschland Ellas
          English España France
          Italia Korea Magyarország
          Nederland  Norge Polska
          Portugal Suomi Sverige
          България

          Certificados de análisis

          CargoNúmero de lote
          70773

          Citas

          Título
        • Wu. L., et al. (2009) Structural Basis for Proteolytic Specificity of the Human Apoptosis-Inducing Granzyme M J. Immunol. 183, 421.
        • Damien Bessière, et al. (2008) Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways. Journal of Biological Chemistry 283, 4352-4363.
        • Nobutaka Funa, Takayoshi Awakawa and Sueharu Horinouchi. (2007) Pentaketide resorcylic acid synthesis by Type III polyketide synthase from Neurospora crassa. Journal of Biological Chemistry 282, 14476-14481.
        • Yongchao Li, Diana C. Irwin and David B. Wilson. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Applied and Enviornmental Microbiology 73, 3165-3172.
        • Jean-Michel Heraud, et al. (2006) Subunit recombinant vaccine protects against monkeypox. Journal of immunology 177, 2552-2564.
        • Stan J. J. Brouns, et al. (2005) Engineering a selectable marker for hyperthermophiles. Journal of Biological Chemistry 280, 11422-11431.
        • Nobutaka Funa, et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. Journal of Biological Chemistry 280, 14514-14523.
        • Ryohei Ishii, et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. Journal of Biological Chemistry 280, 14138-14144.
        • Hongmin Li, et al. (2005) Crystal structures of T cell receptor β chains related to rheumatoid arthritis. Protein Science 14, 3025-3038.
        • Taishi Tsubouchi, et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Journal of Biological Chemistry 280, 18511-18516.
        • Florence Vincent, Gideon J. Davies and James A. Brannigan. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? Journal of Biological Chemistry 280, 19649-19655.
        • Yu-hang Chen, et al. (2004) Structural basis of the α1-β subunit interaction of voltage-gated Ca2+ channels. 429, 675-680.
        • Watchrra Teartasin, et al. (2004) Expression, purification, and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallographica D60, 1137-1138.
        • Shalom D. Goldberg, et al. (2003) Identification of residues critical for catalysis in a class C b-lactamase by combinatorial scanning mutagenesis. Protein Science 12, 1633-1645.
        • Protocolos de usuario

          Cargo
          TB053 Academic and Non-profit Laboratory Assurance Letter
          TB055 pET System Manual

          Mapa vectorial

          Cargo
          TB071VM pET-26b(+) Vector Map

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          Categorías

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors