70773 | pET Expression System 26b - Novagen

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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      pET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 26b
      The pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB071VM.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

      Catalogue Number 70773
      Brand Family Novagen®
      References
      Product Information
      Components
      Fusion tag His•Tag, HSV•Tag
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze No
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Idiomas
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      Certificados de análisis

      CargoNúmero de lote
      70773

      Citas

      Título
    • Wu. L., et al. (2009) Structural Basis for Proteolytic Specificity of the Human Apoptosis-Inducing Granzyme M J. Immunol. 183, 421.
    • Damien Bessière, et al. (2008) Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways. Journal of Biological Chemistry 283, 4352-4363.
    • Nobutaka Funa, Takayoshi Awakawa and Sueharu Horinouchi. (2007) Pentaketide resorcylic acid synthesis by Type III polyketide synthase from Neurospora crassa. Journal of Biological Chemistry 282, 14476-14481.
    • Yongchao Li, Diana C. Irwin and David B. Wilson. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Applied and Enviornmental Microbiology 73, 3165-3172.
    • Jean-Michel Heraud, et al. (2006) Subunit recombinant vaccine protects against monkeypox. Journal of immunology 177, 2552-2564.
    • Stan J. J. Brouns, et al. (2005) Engineering a selectable marker for hyperthermophiles. Journal of Biological Chemistry 280, 11422-11431.
    • Nobutaka Funa, et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. Journal of Biological Chemistry 280, 14514-14523.
    • Ryohei Ishii, et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. Journal of Biological Chemistry 280, 14138-14144.
    • Hongmin Li, et al. (2005) Crystal structures of T cell receptor β chains related to rheumatoid arthritis. Protein Science 14, 3025-3038.
    • Taishi Tsubouchi, et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Journal of Biological Chemistry 280, 18511-18516.
    • Florence Vincent, Gideon J. Davies and James A. Brannigan. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? Journal of Biological Chemistry 280, 19649-19655.
    • Yu-hang Chen, et al. (2004) Structural basis of the α1-β subunit interaction of voltage-gated Ca2+ channels. 429, 675-680.
    • Watchrra Teartasin, et al. (2004) Expression, purification, and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallographica D60, 1137-1138.
    • Shalom D. Goldberg, et al. (2003) Identification of residues critical for catalysis in a class C b-lactamase by combinatorial scanning mutagenesis. Protein Science 12, 1633-1645.
    • Protocolos de usuario

      Cargo
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Mapa vectorial

      Cargo
      TB071VM pET-26b(+) Vector Map

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      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors