MAB351 | Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6

Mouse anti-Glutamate Decarboxylase [GAD] (Catalog Number MAB351) localization of GAD in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Mouse anti-Glutamate Decarboxylase [GAD] (Catalog Number MAB351) localization of GAD in rat hippocam
MAB351
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      Overview

      Ofertas especiales

      Tabla espec. clave

      Reactividad según especiesAplicaciones claveHospedadorFormatoTipo de anticuerpo
      Rata, Ser humanoWB, InmunohistoquímicaRatónPurificadoAnticuerpo monoclonal
      Descripción
      Número de catálogo MAB351
      Marca de familia Chemicon®
      Sinónimos GAD65
      Nombre comercial
      • Chemicon
      Descripción Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6
      Información preliminar Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk et al., 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
      Referencias bibliográficas
      Información del producto
      Formato Purificado
      Especificidad Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain (Gottlieb, et al., 1986; Chang & Gottlieb, 1988). This monclonal antibody can be used for immunohistochemical localization in brain or pancreas. Anti-GAD has also been used to label purified GAD on Western blots (Chang & Gottlieb, 1988).
      Control
      • human brain lysate
      • Rat brain tissue
      Presentación Purified immunoglobulin. Lyophilized from 10 mM potassium phosphate, 70 mM sodium chloride, pH 7.4, 0.02% sodium azide. Reconstitute with 100 μL of sterile water (1 mg/mL).
      Aplicaciones
      Aplicación Anti-Glutamate Decarboxylase Antibody, 65 kDa isoform, clone GAD-6 detects level of Glutamate Decarboxylase & has been published & validated for use in IH & WB.
      Aplicaciones clave
      • Western Blotting
      • Inmunohistoquímica
      Notas de aplicación Immunohistochemistry: ( 1 μg/mL * See protocol)

      Western blot

      Optimal working dilutions must be determined by end user.

      APPLICATION NOTES FOR MAB351

      IMMUNOHISTOCHEMISTRY

      1) Perfuse rats with 100 mM phosphate buffer, pH 7.4 containing 1% paraformaldehyde, 0.34% L-lysine and 0.05% m-periodate (1% PLP).

      2) Postfix brains in 1% PLP for 1-2 hours.

      3) Transfer brains to 100 mM phosphate buffer containing 30% sucrose and gently agitate on a shaker platform at +4°C for 48-60 hours.

      4) Using a sliding microtome, cut 30 mm sections of frozen cerebellum. As the sections are cut, collect them in a vial of cold 100 mM phosphate buffer.

      5) Incubate sections in PBS containing 1.5% normal serum and 0.2% Triton X-100 for 30 minutes.

      6) On a shaker platform, incubate sections with MAB351 (diluted 1 μg/mL in PBS containing 1.5% normal serum and 0.2% Triton X-100) for 12-36 hours at +4°C.

      7) On a shaker platform, rinse sections eight times, 10-15 minutes per rinse, in PBS.

      8) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

      9) Mount sections, dehydrate, and apply coverslips.
      Información biológica
      Especies inmunógenas Purified rat brain glutamic acid decarboxylase.
      Clon GAD-6
      Concentración Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hospedador Ratón
      Especificidad Recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain (Gottlieb, et al., 1986; Chang & Gottlieb, 1988). This monclonal antibody can be used for immunohistochemical localization in brain or pancreas. Anti-GAD has also been used to label purified GAD on Western blots (Chang & Gottlieb, 1988).
      Isotipo IgG2a
      Reactividad según especies Rata Ser humano
      Tipo de anticuerpo Anticuerpo monoclonal
      Número de Entrez Gene
      Resumen de Entrez Gene This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.
      Símbolo de gen
      • EC 4.1.1.15
      • GAD2
      • MGC161607
      • GAD65
      • MGC161605
      • GAD-65
      Método de purificación Protein A purfied
      Número UniProt
      Resumen UniProt FUNCTION: SwissProt: Q05329 # Catalyzes the production of GABA.
      COFACTOR: Pyridoxal phosphate.
      SIZE: 585 amino acids; 65411 Da
      SUBUNIT: Homodimer (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
      PTM: Phosphorylated; which does not affect kinetic parameters or subcellular location. & Palmitoylated; which is required for presynaptic clustering.
      SIMILARITY: SwissProt: Q05329 ## Belongs to the group II decarboxylase family.
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Declaración de uso
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Información de almacenamiento y transporte
      Condiciones de almacenamiento Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Información sobre embalaje
      Tamaño material 100 μg
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Ficha datos de seguridad (MSDS)

      Idiomas
      English

      Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE -2519307 2519307
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY - 1998916 1998916
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY - 2070808 2070808
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY - 2189668 2189668
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY - 2275543 2275543
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY - 2326979 2326979
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY - 2365949 2365949
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY -2475646 2475646

      Referencias bibliográficas | 27 Disponible | Ver todas las referencias

      Visión general referenciasPub Med ID
      Thalamocortical dysfunction and thalamic injury after asphyxial cardiac arrest in developing rats.
      Michael Shoykhet,Daniel J Simons,Henry Alexander,Christina Hosler,Patrick M Kochanek,Robert S B Clark
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 2012

      Mostrar resumen
      22492052
      Reduced glutamate decarboxylase 65 protein within primary auditory cortex inhibitory boutons in schizophrenia.
      Caitlin E Moyer,Kristen M Delevich,Kenneth N Fish,Josephine K Asafu-Adjei,Allan R Sampson,Karl-Anton Dorph-Petersen,David A Lewis,Robert A Sweet
      Biological psychiatry 72 2012

      Mostrar resumen
      22624794
      Salubrinal, an endoplasmic reticulum stress blocker, modulates sleep homeostasis and activation of sleep- and wake-regulatory neurons.
      Methippara, M, et al.
      Neuroscience, 209: 108-18 (2012) 2012

      Mostrar resumen
      22387272
      Compensation of depleted neuronal subsets by new neurons in a local area of the adult olfactory bulb.
      Murata K, Imai M, Nakanishi S, Watanabe D, Pastan I, Kobayashi K, Nihira T, Mochizuki H, Yamada S, Mori K, Yamaguchi M.
      The Journal of neuroscience : the official journal of the Society for Neuroscience 31 10540-57 2011

      Mostrar resumen
      21775599
      Nitric oxide signaling modulates synaptic transmission during early postnatal development.
      Csaba Cserép,András Szonyi,Judit M Veres,Beáta Németh,Eszter Szabadits,Jan de Vente,Norbert Hájos,Tamás F Freund,Gábor Nyiri
      Cerebral cortex (New York, N.Y. : 1991) 21 2011

      Mostrar resumen Artículo Texto completo
      21282319
      Interneurons in the developing human neocortex.
      Nada Zecevic,Frances Hu,Igor Jakovcevski
      Developmental neurobiology 71 2011

      Mostrar resumen
      21154907
      Complementary distribution of glutamatergic cerebellar and GABAergic basal ganglia afferents to the rat motor thalamic nuclei.
      Eriko Kuramoto,Fumino Fujiyama,Kouichi C Nakamura,Yasuhiro Tanaka,Hiroyuki Hioki,Takeshi Kaneko
      The European journal of neuroscience 33 2011

      Mostrar resumen
      21073550
      Synaptic reorganisation of the medial amygdala during puberty.
      Cooke BM
      J Neuroendocrinol 23 65-73. doi 2011

      Mostrar resumen
      20874778
      Evaluation of AAV-Mediated Expression of Chop2-GFP in the Marmoset Retina.
      Ivanova E, Hwang GS, Pan ZH, Troilo D
      Invest Ophthalmol Vis Sci 2010

      Mostrar resumen
      20484599
      Reduced cortical BDNF expression and aberrant memory in Carf knock-out mice.
      Kelli A McDowell,Ashley N Hutchinson,Sarah J E Wong-Goodrich,Matthew M Presby,Dan Su,Ramona M Rodriguiz,Krystal C Law,Christina L Williams,William C Wetsel,Anne E West
      The Journal of neuroscience : the official journal of the Society for Neuroscience 30 2010

      Mostrar resumen Artículo Texto completo
      20519520

      Ficha técnica

      Cargo
      MOUSE ANTI-GLUTAMIC ACID DECARBOXYLASE MONOCLONAL ANTIBODY

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