MAB3068 | Anti-Connexin 43 Antibody, CT

Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CONNEXIN 43 (1:500 dilution of this lot).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates protein CONNEXIN 43 (~43 kDa).
Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF
MAB3068
100 µg  
Recuperando precio...
No pudo obtenerse el precio
Minimum Quantity needs to be mulitiple of
Al finalizar el pedido Más información
Ahorró ()
 
Solicitar precio
Disponibilidad a confirmar Disponibilidad a confirmar
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    Remaining : Will advise
      Se avisará

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

         CompararClick To Print This Page

      Overview

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Reactividad según especies Aplicaciones clave Hospedador Formato Tipo de anticuerpo
      Rata, Canina, Pollo, Ser humano, RatónWB, ELISA, Inmunocitoquímica, Immunofluorescencia, Inmunohistoquímica, InmunoprecipitaciónRatónPurificadoAnticuerpo monoclonal
      Descripción
      Número de catálogo MAB3068
      Marca de familia Chemicon®
      Nombre comercial
      • Chemicon
      Descripción Anti-Connexin 43 Antibody, CT
      Alternate Names
      • gap junction protein, alpha-like
      • Gap junction 43 kDa heart protein
      • connexin 43
      • gap junction protein, alpha 1, 43kDa
      • gap junction protein, alpha 1, 43kDa (connexin 43)
      • 43kDa
      • 43kDa (connexin 43)
      Información preliminar Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.
      Referencias bibliográficas
      Información del producto
      Formato Purificado
      Control
      • Rat brain lysate
      Presentación Purified mouse monoclonal IgG1 liquid in buffer containing aqueous buffered solution containing 50% glycerol, BSA and < 0.09% sodium azide
      Aplicaciones
      Aplicación Detect Connexin 43 using this Anti-Connexin 43 Antibody, C-terminus validated for use in ELISA, IC, IF, IH, IP & WB.
      Aplicaciones clave
      • Western Blotting
      • ELISA
      • Inmunocitoquímica
      • Immunofluorescencia
      • Inmunohistoquímica
      • Inmunoprecipitación
      Notas de aplicación Western blot:
      1:500 Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse fibroblasts.

      ELISA:
      A previous lot of this antibody was used in Enzyme-linked Immunosorbent Assay.

      Immunofluorescence:
      A previous lot of this antibody was used in Enzyme-linked Immunosorbent Assay.

      Immunohistochemistry:
      (2% paraformaldehyde fixation) of 12 μm cryosections of canine Purkinje fibers. 1:250-1:1000 dilution of a previous lot was used with incubation for 16 hours at 4ºC prior to fluorescence detection [Circulation Res. (1993) 72: 1124].

      Immunocytochemistry (acetone fixation):
      1:50-1:300 dilution of a previous lot was used for fluorescence detection (see Mol. Cell Biol (1990) 10: 1754). More sensitive methods of detection will permit increased antibody dilution.

      Immunoprecipitation:
      A previous lot of this antibody was used in immunoprecipitation.

      Optimal working dilutions must be determined by end user.
      Información biológica
      Especies inmunógenas A synthetic peptide corresponding to positions 252-270 of the native sequence from rat cardiac connexin 43 [NH2-GDLSPSKDCGSPK YAYFNGK-COOH]
      Epítopo C-terminus
      Clon RN26
      Concentración Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hospedador Ratón
      Especificidad Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse fibroblasts. Also effective for detection of Cx43 in human fibroblasts, rat brain, canine ventricular myocytes, and goldfish astrocytes. The molecular weight range (43-37 kDa) detected in Western blot of mouse 3T3 fibroblasts likely represents post-translational modifications of a 43 kDa primary translation product [Mol. Cell Biol. (1990) 10: 1754].
      Isotipo IgG1
      Reactividad según especies Rata Canina Pollo Ser humano Ratón
      Species Reactivity Note Human, rat, canine, mouse, chick.
      Tipo de anticuerpo Anticuerpo monoclonal
      Número de Entrez Gene
      Resumen de Entrez Gene This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.
      Símbolo de gen
      • CX43
      • GJAL
      • Cx43
      • ODD
      • DFNB38
      • ODDD
      • ODOD
      • SDTY3
      • Connexin-43
      • GJA1
      Método de purificación Protein A purfied
      Número UniProt
      Resumen UniProt FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
      SIZE: 382 amino acids; 43008 Da
      SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
      TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea.
      DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
      SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Aseguramiento de calidad Routinely evaluated by Western Blot on Mouse brain lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected CONNEXIN 43 on 10 μg of Mouse brain lysates.
      Declaración de uso
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Información de almacenamiento y transporte
      Condiciones de almacenamiento Stable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Información sobre embalaje
      Cantitad 100 µg
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Ficha datos de seguridad (MSDS)

      Idiomas
      English

      Certificados de análisis

      CargoNúmero de lote
      Anti-Connexin 43, C-terminus2483213
      Anti-Connexin 43, C-terminus - 23431332343133
      Anti-Connexin 43, C-terminus - 23922432392243
      Anti-Connexin 43, C-terminus - 19946671994667
      Anti-Connexin 43, C-terminus - 20909182090918
      Anti-Connexin 43, C-terminus - 21919342191934
      Anti-Connexin 43, C-terminus - 22877082287708
      Anti-Connexin 43, C-terminus - JBC1766826JBC1766826
      Anti-Connexin 43, C-terminus - LV1459882LV1459882
      Anti-Connexin 43, C-terminus - LV1519578LV1519578
      Anti-Connexin 43, C-terminus - NG1719692NG1719692
      Anti-Connexin 43, C-terminus - NG1843011NG1843011
      Anti-Connexin 43, C-terminus -25673162567316
      Anti-Connexin 43, C-terminus -26063512606351

      Referencias bibliográficas | 32 Disponible | Ver todas las referencias

      Visión general referenciasPub Med ID
      Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.
      Joanna Gemel,Tasha K Nelson,Janis M Burt,Eric C Beyer
      The Journal of membrane biology 245 2012

      Mostrar resumen
      22729648
      Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance.
      Megan L McCain,Thomas Desplantez,Nicholas A Geisse,Barbara Rothen-Rutishauser,Helene Oberer,Kevin Kit Parker,Andre G Kleber
      American journal of physiology. Heart and circulatory physiology 302 2012

      Mostrar resumen
      22081700
      Connexin-26 is a key factor mediating gemcitabine bystander effect.
      Garcia-Rodríguez L, Pérez-Torras S, Carrió M, Cascante A, García-Ribas I, Mazo A, Fillat C
      Mol Cancer Ther 10 505-17. 2011

      Mostrar resumen
      21388975
      E-cadherin contributes to the bystander effect of TK/GCV suicide therapy and enhances its antitumoral activity in pancreatic cancer models.
      L Garcia-Rodríguez,D Abate-Daga,A Rojas,J R González,C Fillat
      Gene therapy 18 2011

      Mostrar resumen
      20720574
      Influence of v5/6-his tag on the properties of gap junction channels composed of connexin43, connexin40 or connexin45.
      Desplantez T, Halliday D, Dupont E, Severs NJ, Weingart R
      J Membr Biol 240 139-50 2011

      Mostrar resumen
      21424225
      Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits.
      Helga Reinshagen,Claudia Auw-Haedrich,Ruediger V Sorg,Daniel Boehringer,Philipp Eberwein,Johannes Schwartzkopff,Rainer Sundmacher,Thomas Reinhard
      Acta ophthalmologica 89 2011

      Mostrar resumen
      20039850
      Changes of connexin43 expression in non-pregnant porcine myometrium correlate with progesterone concentration during oestrous cycle.
      J Karasinski,J Galas,D Semik,A Fiertak,B Bilinska,W M Kilarski
      Reproduction in domestic animals = Zuchthygiene 45 2010

      Mostrar resumen
      20149142
      Hippocampal seizures alter the expression of the pannexin and connexin transcriptome.
      Mylvaganam S, Zhang L, Wu C, Zhang ZJ, Samoilova M, Eubanks J, Carlen PL, Poulter MO
      J Neurochem 112 92-102. Epub 2009 Oct 13. 2010

      Mostrar resumen
      19840216
      Activator of G protein signaling 8 (AGS8) is required for hypoxia-induced apoptosis of cardiomyocytes: role of G betagamma and connexin 43 (CX43).
      M Sato, Q Jiao, T Honda, R Kurotani, E Toyota, S Okumura, T Takeya, S Minamisawa, SM Lanier, Y Ishikawa
      The Journal of biological chemistry 284 31431-40 2009

      Mostrar resumen Artículo Texto completo
      19723622
      ZO-1 is required for protein kinase C gamma-driven disassembly of connexin 43.
      Vladimir Akoyev,Dolores J Takemoto
      Cellular signalling 19 2007

      Mostrar resumen Artículo Texto completo
      17210245

      Ficha técnica

      Cargo
      Anti-Connexin 43, C-terminus - Data Sheet