EZGLP1T-36K | Multi Species GLP-1 Total ELISA, EZGLP1T-36K

EZGLP1T-36K
96-well plate  
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      Overview

      Replacement Information

      Tabla espec. clave

      Analitos disponibles Reactividad según especies Aplicaciones clave
      Glucagon Like Peptide-1Rata, Ser humano, RatónELISA
      Descripción
      Número de catálogo EZGLP1T-36K
      Descripción Multi Species GLP-1 Total ELISA, EZGLP1T-36K
      Información preliminar Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.
      Referencias bibliográficas
      Información del producto
      Especificidad The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.
      Precisión (%)
      • Inter-assay:< 12%
      • Intra-assay: < 5%
      Aplicaciones
      Aplicación This Multi Species GLP-1 Total ELISA, EZGLP1T-36K is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.
      Aplicaciones clave
      • ELISA
      Notas de aplicación This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
      Información biológica
      Especificidad The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.
      Reactividad según especies Rata Ser humano Ratón
      Analitos disponibles
      • Glucagon Like Peptide-1
      Reactividad cruzada GLP-1 is conserved in almost all mammals.
      Número de Entrez Gene
      Resumen de Entrez Gene The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
      Símbolo de gen
      • glucagon
      • GCG
      • GRPP
      • GLP1
      • GLP2
      Número UniProt
      Resumen UniProt FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
      SIZE: 180 amino acids; 20909 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
      PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
      SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
      MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
      Precisión
      • Linearity 90 - 132%
      • Recovery: 90 - 110%
      Información fisicoquímica
      Sensibilidad
      • MinDC is 1.5 pM
      Intervalo de curvas patrón
      • 4.1 – 1000 pM
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Información de almacenamiento y transporte
      Información sobre embalaje
      Cantitad 96-well plate
      Información de transporte
      Información complementaria
      Especificaciones