Unidades de filtración Stericup®

El sistema de filtración a vacío Stericup combina una unidad de filtración Steritop con un recipiente de recogida para procesar y almacenar volúmenes de 150 ml a 1.000 ml.

El sistema de filtración a vacío Stericup combina una unidad de filtración Steritop con un recipiente de recogida para procesar y almacenar volúmenes de 150 ml a 1.000 ml.

El nuevo diseño de recipiente con agarre sencillo y su compacto perfil mejoran la estabilidad durante la filtración, facilitando el agarre del recipiente de recogida y haciendo la unidad Stericup idónea para usar en campanas de flujo laminar.

Como ventaja adicional, la base del recipiente de recogida está ligeramente hacia dentro, lo que permite apilar los recipientes tapados para su conveniente almacenamiento.

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Unidades con membrana Millipore Express (PES) de 0,1 µm de alto caudal y baja adsorción proteica para la esterilización de soluciones acuosas y eliminación de micoplasma; se suministran estériles Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Capacidad del embudo (ml)icon Receptoricon Tamaño de envaseicon
SCVPU02REUnidad de filtración Stericup-VP; 0,10 µm, poliétersulfona, 250 ml, esterilizada por radiación250Botella con tapa, 250 ml12 Precios y disponibilidad
SCVPU11REUnidad de filtración Stericup-VP; 0,10 µm, poliétersulfona, 1000 ml, esterilizada por radiación1000Botella con tapa, 1.000 ml12 Precios y disponibilidad

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Unidades con membrana Millipore Express PLUS (PES) de 0,22 µm de alto caudal y baja adsorción proteica para la esterilización de soluciones acuosas; se suministran estériles* Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Capacidad del embudo (ml)icon Receptoricon Tamaño de envaseicon
SCGPU01REUnidad de filtración Stericup-GP; 0,22 µm, poliétersulfona, 150 ml, esterilizada por radiación150Botella con tapa, 150 ml12 Precios y disponibilidad
SCGPU02REUnidad de filtración Stericup-GP; 0,22 µm, poliétersulfona, 250 ml, esterilizada por radiación250Botella con tapa, 250 ml12 Precios y disponibilidad
SCGPU05REUnidad de filtración Stericup-GP; 0,22 µm, poliétersulfona, 500 ml, esterilizada por radiación500Botella con tapa, 500 ml12 Precios y disponibilidad
SCGPU10REUnidad de filtración Stericup-GP; 0,22 µm, poliétersulfona, 500/1.000 ml, esterilizada por radiación500Botella con tapa, 1.000 ml12 Precios y disponibilidad
SCGPU11REUnidad de filtración Stericup-GP; 0,22 µm, poliétersulfona, 1.000/1.000 ml, esterilizada por radiación1000Botella con tapa, 1.000 ml12 Precios y disponibilidad

* Estos productos han sido ensayados para usar en aplicaciones de investigación con células madre. Para determinar sus efectos en el crecimiento o diferenciación de células madre de ratón, se han utilizado tres lotes de unidades Stericup-GP para filtrar medios de cultivo con LIF. Una vez filtrado, este medio se utilizó para pasar las células madre de ratón cinco veces a fin de verificar que la filtración con las unidades Stericup-GP no afecta a la pluripotencialidad de las células madre de ratón.

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Unidades con membrana Durapore (PVDF) de 0,22 µm de muy baja adsorción proteica para esterilización de soluciones acuosas; se suministran estériles Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Capacidad del embudo (ml)icon Receptoricon Tamaño de envaseicon
SCGVU01REUnidad de filtración Stericup-GV; 0,22 µm, PVDF, 150 ml, esterilizada por radiación150Botella con tapa, 150 ml12 Precios y disponibilidad
SCGVU02REUnidad de filtración Stericup-GV; 0,22 µm, PVDF, 250 ml, esterilizada por radiación250Botella con tapa, 250 ml12 Precios y disponibilidad
SCGVU05REUnidad de filtración Stericup-GV; 0,22 µm, PVDF, 500 ml, esterilizada por radiación500Botella con tapa, 500 ml12 Precios y disponibilidad
SCGVU10REUnidad de filtración Stericup-GV; 0,22 µm, PVDF, 500/1.000 ml, esterilizada por radiación500Botella con tapa, 1.000 ml12 Precios y disponibilidad
SCGVU11REUnidad de filtración Stericup-GV; 0,22 µm, PVDF, 1.000/1.000 ml, esterilizada por radiación1000Botella con tapa, 1.000 ml12 Precios y disponibilidad

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Unidades con membrana Durapore (PVDF) de 0,45 µm de muy baja adsorción proteica para clarificación de soluciones acuosas; se suministran estériles Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Capacidad del embudo (ml)icon Receptoricon Tamaño de envaseicon
SCHVU01REUnidad de filtración Stericup-HV; 0,45 µm, PVDF, 150 ml, esterilizada por radiación150Botella con tapa, 150 ml12 Precios y disponibilidad
SCHVU02REUnidad de filtración Stericup-HV; 0,45 µm, PVDF, 250 ml, esterilizada por radiación250Botella con tapa, 250 ml12 Precios y disponibilidad
SCHVU05REUnidad de filtración Stericup-HV; 0,45 µm, PVDF, 500 ml, esterilizada por radiación500Botella con tapa, 500 ml12 Precios y disponibilidad
SCHVU11REUnidad de filtración Stericup-HV; 0,45 µm, PVDF, 1000/1.000 ml, esterilizada por radiación1000Botella con tapa, 1.000 ml12 Precios y disponibilidad

* These products have been tested and validated for use in stem cell research applications. To determine their effects on Mouse Stem Cell growth or differentiation, three lots of Stericup-GP devices were used to filter media with LIF. Once filtered, this media was used to passage mouse stem cells five times to verify that Stericup-GP filtration does not impact pluripotency of mouse stem cells.

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    Documentation

    Folleto

    Cargo
    Advanced cell culture systems for every challenge.
    Precise cell assays start with Stericup® media filtration
    Simply better membrane technology, simply better Stericup® filters.
    Sterile filtration you can trust.

    Ficha técnica

    Cargo
    Maximize the Performance of your UHPLC System
    Stericup and Steritop Vacuum Filter Cups

    Póster

    Cargo
    Laboratory Filtration Membranes, Materials and Formats

    Preguntas frecuentes

    PreguntaRespuesta
    Can I purchase extra receiver flasks for the Stericups separately? Yes, the catalog number is SC00B10RE.
    Can you freeze Stericup units? You can successfully freeze and store many aqueous solutions ( such as culture media ) at temperatures to -20C ( -4F ). We strongly recommend that you run a trial under actual conditions to test for suitability of the bottles for frozen storage.
    Do you have a filter for 100% serum? Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.
    I want to sterilize 100% ethanol. Are any of the Stericups or Steritops compatible with ethanol? The funnel and receiver flask is made of polystyrene. Any molded polystyrene will craze in pure ethanol and could structurally fail depending on the forces being applied to it. The funnel could handle the short exposure time but the receiver would craze eventually. Therefore we would not recommend the Stericup with 100% ethanol. The Steritop, if you keep the exposure time to the funnel minimal, should be fine. As you dilute the alcohol the compatibility improves.

    Both Express and Durapore membranes are compatible. MCE is not.
    I will be filtering tissue culture media with your Stericup/top and am concerned that wetting agents in the filter could effect my cells. Which membrane type would you recommend to avoid this? All of Millipore's Tissue Culture Media filtration sterilization devices contain membranes that are wetting agent (surfactant ) free.
    When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening? Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
    What is the diameter of the membrane in the Stericup and Steritop units? All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
    Should I filter my acrylamide solution prior to gel casting? Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things.
    1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and
    2. It will aid in degassing, which will promote efficient polymerization of the gel.

    Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane.
    It sounds like air is leaking into my Stericup, what should I do? First make sure that the top is securely fastened to the base. Discontinue use if there appears to be any cracks on the apparatus.
    What Stericup would you recommend to remove mycoplasma? The Stericup VP ontains an 0.1um Express membrane. The VP membrane has been validated for the removal of mycoplasma.
    What is the expiration date on Stericup and Steritop units? The expiration date, which specifies product sterility, for the Stericup and Steritop products is three years from the date of manufacture.
    What type of flow rates can I expect with the Stericups and Steritops? For typical non -serum tissue culture media you can expect flow rates from 1-1.5 lpm.
    Are Stericup/tops compatible with formamide? Yes. Formamide is probably the least organic compound. It's almost like water. So it would be compatible with everything. The potential mistake is mistaking formamide for dimethylformamide, which is a powerful organic solvent.
    Can I sterile filter my 10% acetone, 90% water solution through a Stericup with the Durapore membrane? The membrane will be OK since it is well supported in these configurations ( Durapore is not compatible with 100% acetone). The molded plastic components (polystyrene/polyethylene) may have a tendency to develop microcracks, or crazing, due to the acetone being able to releave the stresses from the molding process. If it does craze this will reduce the strength of the molded parts. Crazing would be instantaneous with high levels of acetone. At 10% it is difficult to say. If under a strong vacuum the crazed part could even fail.
    Are Stericup and Steritop units autoclavable? No. The highest temperature that the product can be used at is 50C ( 122F ).
    Can I bubble point my Stericup/top? No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.
    What diameter prefilter is used in the Stericup? We recommend a 75mm diameter. Even though the membrane has a 70mm diameter, there is a very thin lip of plastic that seals it in place in the funnel. The 75mm gives it a nice snug fit.

    Inside the cup there is also a small tab of plastic just above the filter designed for you to slip the prefilter under. This eliminates the prefilter floating up when you add your solution.
    Can I filter 100% serum with the Stericup? Yes. The Express membrane is the highest throughput membrane available on the market today.
    For tissue culture media filtration which membrane should I use? The best membrane for this application is the Millipore Express membrane. It was designed specifically to filter tissue culture media with serum. It is low protein binding, has fast flow and high throughput (which is also called filter capacity).
    Is the Express membrane surfactant free? Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.
    What is the typical holdup volume for Stericup. The hold up volume is about 6 ml. If you give the unit a slight shake, you can reduce the holdup volume by about 60% to 2.4 ml. Solutions containing protein have a slightly higher holdup volume, about 7 ml, again reduced to less than half by a slight shake.
    Why use a vacuum driven filter rather than a pressure driven filter? Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy!
    Which pre-filter should I be using with my Stericup or Steritop unit? You should use an AP20 75mm pre-filter (AP2007500). To help keep the filter from floating in the solution to be filtered, insert the pre-filter under the pre-filter tab inside the Stericup or Steritop.
    What is the thread size on the Stericup units? The thread size on the Stericup units is 45mm.

    Manuales del usuario

    Cargo
    Stericup and Steritop Filtration and Storage Systems

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    Categorías

    Life Science Research > Cell Culture and Systems > Sterile Filtration Devices

    C3240El sistema de filtración a vacío Stericup combina una unidad de filtración Steritop con un recipiente de recogida para procesar y almacenar volúmenes de 150 ml a 1.000 ml.
    Materiales
    Dispositivo Poliestireno
    Membrana Como se indica en la tabla
    Superficie de filtración, cm2 40
    Volumen de proceso, ml 150 a 1000
    Temperatura operativa máxima, °C 45
    Método de esterilización Radiación gamma
    Prefiltro
    Para soluciones difíciles de filtrar, puede colocarse un prefiltro de fibra de vidrio tipo AP20 en el embudo sobre la membrana y asegurarlo con una pestaña conveniente. Los prefiltros se deben pedir por separado: referencia AP20 075 00, 100/pq.