ABC458 | Anti-Thymidylate Kinase/TMPK Antibody

Western Blotting Analysis: Representative lot data. Jurkat cell lysate was probed with Cat. No. ABC458, Anti-Thymidylate Kinase/TMPK (2 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Thymidylate Kinase/TMPK (~24 kDa).
Western Blotting Analysis: Representative lot data. Jurkat cell lysate was probed with Cat. No. ABC4
ABC458
100 µg  
Recuperando precio...
No pudo obtenerse el precio
Minimum Quantity needs to be mulitiple of
Al finalizar el pedido Más información
Ahorró ()
 
Solicitar precio
Disponibilidad a confirmar Disponibilidad a confirmar
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    Remaining : Will advise
      Se avisará

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

         CompararClick To Print This Page

      Overview

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Reactividad según especies Aplicaciones clave Hospedador Formato Tipo de anticuerpo
      Ser humanoInmunoprecipitación, WBConejoPurificadoAnticuerpo policlonal
      Descripción
      Número de catálogo ABC458
      Descripción Anti-Thymidylate Kinase/TMPK Antibody
      Alternate Names
      • dTMP kinase
      • Thymidylate kinase
      Información preliminar TMPK, also known as dTMP kinase, and encoded by the gene DTYMK/CDC8/TMPK/TYMK, is a key enzyme for pyrimidine synthesis that catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form thymidine 5'-diphosphate (dTDP). TMPK has important functions in cell proliferation and DNA replication, and the synthesis of dTDP is unique because it requires TMPK. All other dNDPs are directly produced by ribonucleotide reductase (RNR). Thus, TMPK is well recognized as a potential drug target for many diseases and disease agents including viruses. TMPK is currently a target of anti-HIV nucleoside therapy, and TMPK is also a validated target for antibiotic development against resistance strains of bacteria and parasites, and research demonstrates that it may also be a good target for the anticancer agent doxorubicin cancer cells. Expressed by virtually all cells it is localized to cell cytoplasm with only traces found in Golgi, mitochondria and nuclei.
      Referencias bibliográficas
      Información del producto
      Formato Purificado
      Presentación Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Aplicaciones
      Aplicación Anti-Thymidylate Kinase/TMPK Antibody is a rabbit polyclonal antibody, validated for use in western blotting & IP.
      Aplicaciones clave
      • Inmunoprecipitación
      • Western Blotting
      Notas de aplicación Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MDA-MB231, MCF-7, H184B5F5/M10 cell lysates (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunprecipitated Thymidylate Kinase/TMPK from MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).
      Información biológica
      Especies inmunógenas GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.
      Concentración Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hospedador Conejo
      Reactividad según especies Ser humano
      Tipo de anticuerpo Anticuerpo policlonal
      Número de Entrez Gene
      Símbolo de gen
      • TYMK
      • DTYMK
      • CDC8
      • TMPK
      Método de purificación Protein A purified
      Número UniProt
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Aseguramiento de calidad Evaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected Thymidylate Kinase/TMPK in 10 µg of Jurkat cell lysate.
      Declaración de uso
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Información de almacenamiento y transporte
      Condiciones de almacenamiento Stable for 1 year at 2-8°C from date of receipt.
      Información sobre embalaje
      Cantitad 100 µg
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Certificados de análisis

      CargoNúmero de lote
      Anti-Thymidylate Kinase/TMPK - Q2341232Q2341232

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability.
      Ke, Po-Yuan, et al.
      Genes Dev., 19: 1920-33 (2005) 2005

      Mostrar resumen
      16103219