17-700 | Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

PCR Analysis of RIP (Fold Enrichment):
RIP was performed using HeLa cell lysate and either anti-SNRNP70 or Normal Rabbit IgG as the immunoprecipitating antibody. Purified RNA was then analyzed by qRT-PCR using RIP Primers specific for the U1 snRNA.
PCR Analysis of RIP (Fold Enrichment):
RIP was performed using HeLa cell lysate and either anti
17-700
12 assays  RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
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      Overview

      Replacement Information

      Tabla espec. clave

      Key Applications
      RIP
      Description
      Catalogue Number 17-700
      Brand Family Upstate
      Trade Name
      • Magna RIP
      • Upstate
      Description Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
      Overview RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).

      Features & Benefits:
      -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
      -RNAse inhibitors and RNAse-free reagents
      -Negative controls
      Alternate Names
      • Magnetic RIP kit
      Background Information Gene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
      products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
      References
      Product Information
      Components
      • Magnetic Beads Protein A/G
      • Nuclease free water
      • Proteinase K (10 mg/mL)
      • RNase Inhibitor
      • Protease Inhibitor Cocktail 200X
      • Rabbit IgG Purified
      • Normal Mouse IgG
      • Precipitate Enhancer
      • Salt Solution II
      • Salt Solution I
      • 10% SDS
      • 0.5 M EDTA
      • RIP Lysis Buffer
      • RIP Wash Buffer
      Presentation Two boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
      Applications
      Application RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
      Packaging Information
      Material Size 12 assays
      Material Package RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Title

      Material Safety Data Sheet (MSDS)  

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      MicroRNA-466l Upregulates IL-10 Expression in TLR-Triggered Macrophages by Antagonizing RNA-Binding Protein Tristetraprolin-Mediated IL-10 mRNA Degradation.
      Ma, Feng, et al.
      Journal of immunology (Baltimore, Md. : 1950), (2010) 2010

      Mostrar resumen
      20410487
      Advances in RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray profiling.
      Baroni, Timothy E, et al.
      Methods Mol. Biol., 419: 93-108 (2008) 2008

      Mostrar resumen
      18369977
      Ribonomic and short hairpin RNA gene silencing methods to explore functional gene programs associated with tumor growth arrest.
      Baroni, Timothy E, et al.
      Methods Mol. Biol., 383: 227-44 (2007) 2007

      Mostrar resumen
      18217689
      RIP-Chip: the isolation and identification of mRNAs, microRNAs and protein components of ribonucleoprotein complexes from cell extracts.
      Keene, Jack D, et al.
      Nat Protoc, 1: 302-7 (2006) 2006

      Mostrar resumen
      17406249
      Gene expression analysis of messenger RNP complexes.
      Penalva, Luiz O F, et al.
      Methods Mol. Biol., 257: 125-34 (2004) 2004

      Mostrar resumen
      14770002
      Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays.
      Tenenbaum, Scott A, et al.
      Methods, 26: 191-8 (2002) 2002

      Mostrar resumen
      12054896
      Identifying mRNA subsets in messenger ribonucleoprotein complexes by using cDNA arrays.
      Tenenbaum, S A, et al.
      Proc. Natl. Acad. Sci. U.S.A., 97: 14085-90 (2000) 2000

      Mostrar resumen
      11121017
      Soluble and particulate metals in the Po River: factors affecting concentrations and partitioning.
      M Pettine,M Camusso,W Martinotti,R Marchetti,R Passino,G Queirazza
      The Science of the total environment 145 1994

      Mostrar resumen
      8023132

      Folleto

      Cargo
      RNA-Binding Protein Immunoprecipitation

      Manuales del usuario

      Cargo
      Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

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      Categorías

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Kits