17-218 | Ras Activation Assay Kit

Affinity Precipitation/Immunoblot Analysis: 1 milligram of HeLa cell lysate was loaded with GDP (lane 1) or with GTPγS (lane 2) then incubated with Raf-1 RBD, agarose. Subsequent immunoblotting was performed with 1μg/ml of Anti-Ras. Arrow indicates Ras (~21kDa).
Affinity Precipitation/Immunoblot Analysis: 1 milligram of HeLa cell lysate was loaded with GDP (lan
17-218
1 kit  Kit capacity: 30 assays
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      Overview

      Tabla espec. clave

      Aplicaciones clave
      Ensayo enzimático
      Descripción
      Número de catálogo 17-218
      Replaces SGT435
      Marca de familia Upstate
      Nombre comercial
      • Upstate
      Descripción Ras Activation Assay Kit
      Referencias bibliográficas
      Información del producto
      Componentes Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168) Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278) Anti-Ras, clone RAS10 (Cat.# 05-516) 100X GTPγS, 10mM (Cat.# 20-176) 100X GDP, 100mM (Cat.# 20-177)
      Aplicaciones
      Aplicaciones clave
      • Ensayo enzimático
      Información biológica
      Número de Entrez Gene
      Resumen de Entrez Gene Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM]
      Símbolo de gen
      • RRAS3
      • MRAS
      • M-RAs
      • M-RAS
      • FLJ42964
      • R-RAS3
      Número UniProt
      Resumen UniProt FUNCTION: SwissProt: O14807 # May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
      SIZE: 208 amino acids; 23846 Da
      SUBUNIT: Interacts with RGL3 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
      TISSUE SPECIFICITY: Expression highly restricted to the brain and heart.
      SIMILARITY: SwissProt: O14807 ## Belongs to the small GTPase superfamily. Ras family.
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Aseguramiento de calidad This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10
      Declaración de uso
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Información de almacenamiento y transporte
      Información sobre embalaje
      Tamaño material 1 kit
      Material de embalaje Kit capacity: 30 assays
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Ficha datos de seguridad (MSDS)

      Idiomas
      English

      Certificados de análisis

      CargoNúmero de lote
      Ras Activation Assay Kit (non-radioactive) - 2390546 2390546
      Ras Activation Assay Kit (non-radioactive) - 1959294 1959294
      Ras Activation Assay Kit (non-radioactive) - 2166054 2166054
      Ras Activation Assay Kit (non-radioactive) - 2210394 2210394
      Ras Activation Assay Kit (non-radioactive) - 2281660 2281660
      Ras Activation Assay Kit (non-radioactive) - 2329806 2329806
      Ras Activation Assay Kit (non-radioactive) -2484190 2484190
      Ras Activation Assay Kit - 2430405 2430405
      Ras Activation Assay Kit - 0606032766 0606032766
      Ras Activation Assay Kit - 0609039663 0609039663
      Ras Activation Assay Kit - 0611045665 0611045665
      Ras Activation Assay Kit - 16849 16849
      Ras Activation Assay Kit - 18775 18775
      Ras Activation Assay Kit - 19032 19032
      Ras Activation Assay Kit - 19318 19318
      Ras Activation Assay Kit - 19683 19683
      Ras Activation Assay Kit - 2047789 2047789
      Ras Activation Assay Kit - 2064532 2064532
      Ras Activation Assay Kit - 20653 20653
      Ras Activation Assay Kit - 21074 21074
      Ras Activation Assay Kit - 21375 21375
      Ras Activation Assay Kit - 21955 21955
      Ras Activation Assay Kit - 22838 22838
      Ras Activation Assay Kit - 23501 23501
      Ras Activation Assay Kit - 25027 25027
      Ras Activation Assay Kit - 26220 26220
      Ras Activation Assay Kit - 29904 29904
      Ras Activation Assay Kit - 32019 32019
      Ras Activation Assay Kit - 33439 33439
      Ras Activation Assay Kit - DAM1503342 DAM1503342
      Ras Activation Assay Kit - DAM1556075 DAM1556075
      Ras Activation Assay Kit - DAM1567201 DAM1567201
      Ras Activation Assay Kit - DAM1601770 DAM1601770
      Ras Activation Assay Kit - DAM1692676 DAM1692676
      Ras Activation Assay Kit - DAM1745418 DAM1745418
      Ras Activation Assay Kit - DAM1792809 DAM1792809
      Ras Activation Assay Kit - JBC1370020 JBC1370020
      Ras Activation Assay Kit - JBC1393242 JBC1393242
      Ras Activation Assay Kit - JBC1831438 JBC1831438
      Ras Activation Assay Kit - JBC1903640 JBC1903640
      Ras Activation Assay Kit -2500472 2500472

      Referencias bibliográficas | 24 Disponible | Ver todas las referencias

      Visión general referenciasAplicación Pub Med ID
      The TGFβ Receptor-interacting Protein km23-1/DYNLRB1 Plays an Adaptor Role in TGFβ1 Autoinduction via Its Association with Ras.
      Qunyan Jin,Wei Ding,Kathleen M Mulder
      The Journal of biological chemistry 287 2012

      Mostrar resumen
      22637579
      E4orf1: a novel ligand that improves glucose disposal in cell culture.
      Emily J Dhurandhar,Olga Dubuisson,Nazar Mashtalir,Rashmi Krishnapuram,Vijay Hegde,Nikhil V Dhurandhar
      PloS one 6 2011

      Mostrar resumen Artículo Texto completo
      21886789
      Effects of estrogen on stress-induced premature senescence of vascular smooth muscle cells: A novel mechanism for the \time window theory\ of menopausal hormone therapy.
      Zhu C, Zhang L, Zheng Y, Xu J, Song J, Rolfe BE, Campbell JH
      Atherosclerosis 215 294-300. Epub 2011 Jan 19. 2011

      21300357
      Ligand-specific function of transforming growth factor beta in epithelial-mesenchymal transition in heart development.
      Mohamad Azhar,Raymond B Runyan,Connie Gard,L Philip Sanford,Marian L Miller,Anastasia Andringa,Sharon Pawlowski,Sudarsan Rajan,Thomas Doetschman
      Developmental dynamics : an official publication of the American Association of Anatomists 238 2009

      Mostrar resumen Artículo Texto completo
      19161227
      Activated K-RAS increases polyamine uptake in human colon cancer cells through modulation of caveolar endocytosis.
      Upal K Basu Roy,Nathaniel S Rial,Karen L Kachel,Eugene W Gerner
      Molecular carcinogenesis 47 2008

      Mostrar resumen Artículo Texto completo
      18176934
      Gene silencing for epidermal growth factor receptor variant III induces cell-specific cytotoxicity.
      Yamoutpour, Farnaz, et al.
      Mol. Cancer Ther., 7: 3586-97 (2008) 2008

      Mostrar resumen
      19001441
      RIG1 suppresses Ras activation and induces cellular apoptosis at the Golgi apparatus.
      Tsai, Fu-Ming, et al.
      Cell. Signal., 19: 989-99 (2007) 2007

      Mostrar resumen
      17196792
      RIG1 inhibits the Ras/mitogen-activated protein kinase pathway by suppressing the activation of Ras.
      Tsai, Fu-Ming, et al.
      Cell. Signal., 18: 349-58 (2006) 2006

      Mostrar resumen
      16005186
      Optimization of a nonradioactive method for consistent and sensitive determination of activated K-ras protein.
      Richard J Calvert,Wafa Kammouni,Keith D Kikawa
      Analytical biochemistry 343 2005

      Mostrar resumen
      16018961
      Methylation and inhibition of expression of uPA by the RAS oncogene: divergence of growth control and invasion in breast cancer cells.
      Pouya Pakneshan, Moshe Szyf, Shafaat A Rabbani
      Carcinogenesis 26 557-64 2005

      Mostrar resumen
      15618232

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      05-1072 Anti-Ras Antibody, (K-, H-, N-), clone 9A11.2 Precios y disponibilidad

      Kit Component

       Número de referencia Descripción  
      05-516 Anti-Ras Antibody, clone RAS10 Precios y disponibilidad
      14-278 Ras Assay Reagent (Raf-1 RBD, agarose) Precios y disponibilidad
      20-168 Mg2+ Lysis/Wash Buffer, 5X Precios y disponibilidad
      20-176 100X GTPγS, 10mM Precios y disponibilidad
      20-177 100X GDP, 100mM Precios y disponibilidad

      Familias de productos

      Categorías

      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays