07-175 | Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody

Immunoblot Analysis: 10 μg of untreated (lane 1) or anisomycin treated (30 min) (lane 2) NIH/3T3 cell lysates were resolved by electrophoresis, transferred to Immobilon-P PVDF membrane, and probed with anti-phospho-JNK 1/2 (Thr183/Tyr185, Thr221/Tyr223) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-JNK 1 &2.
Immunoblot Analysis: 10 μg of untreated (lane 1) or anisomycin treated (30 min) (lane 2) NIH/
07-175
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      Overview

      Ofertas especiales

      Tabla espec. clave

      Reactividad según especies Aplicaciones clave Hospedador Formato Tipo de anticuerpo
      Caballo, Ratón, Ser humano, Rata, Canina, Xn, Pollo, BovinaInmunoprecipitación, WB, Ensayo enzimáticoConejoPurificado por afinidadAnticuerpo policlonal
      Descripción
      Número de catálogo 07-175
      Sinónimos protein kinase JNK1 JNK1 alpha protein kinase JNK1 beta protein kinase JUN N-terminal kinase Stress-activated protein kinase JNK1 mitogen-activated protein kinase 8 mitogen-activated protein kinase 8 isoform JNK1 alpha1
      Descripción Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
      Información preliminar JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.
      Referencias bibliográficas
      Información del producto
      Formato Purificado por afinidad
      Especificidad This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .
      Control
      • Untreated and Lambda phosphatase treated RAW264.7 cell lysates
      Presentación Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Aplicaciones
      Aplicación Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.
      Aplicaciones clave
      • Inmunoprecipitación
      • Western Blotting
      • Ensayo enzimático
      Notas de aplicación Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.
      Información biológica
      Especies inmunógenas KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.
      Epítopo Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223
      Concentración Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hospedador Conejo
      Especificidad This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .
      Isotipo IgG
      Reactividad según especies Caballo Ratón Ser humano Rata Canina Xenopus Pollo Bovina
      Species Reactivity Note Demonstrated to react with mouse and human. Predicted to react with rat, canine, xenopus, chicken, bovine, and equine based on 100% sequence homology.
      Tipo de anticuerpo Anticuerpo policlonal
      Número de Entrez Gene
      Resumen de Entrez Gene The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
      Símbolo de gen
      • JNK1A2
      • MAPK8
      • JNK1
      • JNK21B1/2
      • PRKM8
      • JNK-46
      • JNK
      • SAPK1
      Modificaciones
      • Phosphorylation
      Método de purificación Affinity purified
      Número UniProt
      Resumen UniProt FUNCTION: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

      COFACTOR: Magnesium.

      SIZE: 427 amino acids; 48296 Da

      SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).

      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

      PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Aseguramiento de calidad Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.
      Declaración de uso
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Información de almacenamiento y transporte
      Condiciones de almacenamiento Stable for 1 year at 2-8°C from date of receipt.
      Información sobre embalaje
      Tamaño material 200 µg
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Ficha datos de seguridad (MSDS)

      Idiomas
      Deutschland

      Certificados de análisis

      CargoNúmero de lote
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2430491 2430491
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1713218 DAM1713218
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1810820 DAM1810820
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 20316 20316
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2073119 2073119
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2276339 2276339
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2318094 2318094
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2517878 2517878
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 30813 30813
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 32347 32347
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1394789 DAM1394789
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1661070 DAM1661070
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1770310 DAM1770310
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - JBC1361845 JBC1361845

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Activation of c-Jun N-terminal kinase (JNK) during mitosis in retinal progenitor cells.
      Vinicius Toledo Ribas,Bruno Souza Gonçalves,Rafael Linden,Luciana Barreto Chiarini
      PloS one 7 2012

      Mostrar resumen
      22496813
      CD22 regulates adaptive and innate immune responses of B cells.
      Norihito Kawasaki,Christoph Rademacher,James C Paulson
      Journal of innate immunity 3 2011

      Mostrar resumen
      21178327
      Decoy receptor 3, upregulated by Epstein-Barr virus latent membrane protein 1, enhances nasopharyngeal carcinoma cell migration and invasion.
      Cheng-Hsun Ho, Chi-Long Chen, Wing-Yin Li, Chi-Ju Chen, Cheng-Hsun Ho, Chi-Long Chen, Wing-Yin Li, Chi-Ju Chen
      Carcinogenesis 30 1443-51 2009

      Mostrar resumen
      19483191
      Subcellular receptor redistribution and enhanced microspike formation by a Ret receptor preferentially recruiting Dok.
      Anna Stenqvist, T Kalle Lundgren, Matthew J Smith, Ola Hermanson, Gonçalo Castelo-Branco, Tony Pawson, Patrik Ernfors
      Neuroscience letters 435 11-6 2008

      Mostrar resumen
      18353552
      WNT pathways in the neonatal ovine uterus: potential specification of endometrial gland morphogenesis by SFRP2.
      Kanako Hayashi, Thomas E Spencer
      Biology of reproduction 74 721-33 2006

      Mostrar resumen
      16407498