07-001 | Anti-p47-phox Antibody

WB: HL-60 cell lysate was probed with anti-p47-phox (1:2000 dilution). Arrow indicates p47-phox.
07-001
200 µL  
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      Overview

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Reactividad según especies Aplicaciones clave Hospedador Formato Tipo de anticuerpo
      Ser humanoWB, Inmunocitoquímica, InmunoprecipitaciónConejoSueroAnticuerpo policlonal
      Descripción
      Número de catálogo 07-001
      Marca de familia Upstate
      Nombre comercial
      • Upstate
      Descripción Anti-p47-phox Antibody
      Referencias bibliográficas
      Información del producto
      Formato Suero
      Control
      • HL-60 cell lysate
      Presentación Immunoaffinity Purified immunoglobulin in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
      Aplicaciones
      Aplicación Anti-p47-phox Antibody detects level of p47-phox & has been published & validated for use in IC, IP & WB.
      Aplicaciones clave
      • Western Blotting
      • Inmunocitoquímica
      • Inmunoprecipitación
      Información biológica
      Especies inmunógenas GST fusion protein corresponding to full-length human p47-phox
      Concentración Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hospedador Conejo
      Especificidad Recognizes p47 phox.
      Isotipo IgG
      Reactividad según especies Ser humano
      Tipo de anticuerpo Anticuerpo policlonal
      Número de Entrez Gene
      Resumen de Entrez Gene The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
      Símbolo de gen
      • NCF-1
      • NCF1
      • NCF-47K
      • NOXO2
      • p47-phox
      • SH3PXD1A
      • NCF1A
      • p47phox
      Método de purificación Unpurified
      Número UniProt
      Resumen UniProt FUNCTION: SwissProt: P14598 # NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
      SIZE: 390 amino acids; 44683 Da
      SUBCELLULAR LOCATION: Cytoplasm.
      DISEASE:SwissProt: P14598 # Defects in NCF1 are the cause of autosomal cytochrome-b- positive chronic granulomatous disease type 1 (CGD) [MIM:233700]; an autosomal recessive form.
      SIMILARITY: SwissProt: P14598 ## Contains 1 PX (phox homology) domain. & Contains 2 SH3 domains.
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Aseguramiento de calidad routinely evaluated by immunoblot on RIPA lysates from HL-60 cells
      Declaración de uso
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Información de almacenamiento y transporte
      Condiciones de almacenamiento Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Información sobre embalaje
      Cantitad 200 µL
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Certificados de análisis

      CargoNúmero de lote
      Anti-p47-phox (rabbit antiserum) - 21886682188668
      Anti-p47-phox (rabbit antiserum) - 22668252266825
      Anti-p47-phox (rabbit antiserum) - 23283492328349
      Anti-p47-phox (rabbit antiserum) - 23665512366551
      Anti-p47-phox (rabbit antiserum) -24911832491183
      Anti-p47-phox - 1913619136
      Anti-p47-phox - 2104821048
      Anti-p47-phox - 2634726347
      Anti-p47-phox - 2974629746
      Anti-p47-phox - DAM1394795DAM1394795
      Anti-p47-phox - DAM1413939DAM1413939
      Anti-p47-phox - DAM1754397DAM1754397
      Anti-p47-phox - DAM1792490DAM1792490
      Anti-p47-phox - DAM1811082DAM1811082
      Anti-p47-phox - JBC1361869JBC1361869
      Anti-p47-phox - JBC1908439JBC1908439

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Antrocamphin A, an anti-inflammatory principal from the fruiting body of Taiwanofungus camphoratus , and its mechanisms.
      Hsieh YH, Chu FH, Wang YS, Chien SC, Chang ST, Shaw JF, Chen CY, Hsiao WW, Kuo YH, Wang SY
      J Agric Food Chem 58 3153-8. 2010

      Mostrar resumen
      20128588
      Molecular diagnosis of X-linked chronic granulomatous disease in Iran.
      S Teimourian, Z Rezvani, M Badalzadeh, C Kannengiesser, D Mansouri, M Movahedi, E Zomorodian, N Parvaneh, S Mamishi, Z Pourpak, M Moin, S Teimourian, Z Rezvani, M Badalzadeh, C Kannengiesser, D Mansouri, M Movahedi, E Zomorodian, N Parvaneh, S Mamishi, Z Pourpak, M Moin
      International journal of hematology 87 398-404 2008

      Mostrar resumen
      18322777
      A myocardial Nox2 containing NAD(P)H oxidase contributes to oxidative stress in human atrial fibrillation.
      Young M Kim, Tomasz J Guzik, Yin Hua Zhang, Mei Hua Zhang, Hassan Kattach, Chandi Ratnatunga, Ravi Pillai, Keith M Channon, Barbara Casadei
      Circulation research 97 629-36 2005

      Mostrar resumen
      16123335
      Molecular characterization of a superoxide-generating NAD(P)H oxidase in the ventilatory muscles
      Javesghani, D., et al
      Am J Respir Crit Care Med, 165:412-8 (2002) 2002

      Immunoblotting (Western), Immunohistochemistry (Tissue)11818330
      Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: enhancement by angiotensin II
      Pagano, P J, et al
      Proc Natl Acad Sci USA, 94:14483-8 (1997) 1997

      9405639