05-716 | Anti-Myosin Heavy Chain Antibody, clone A4.1025

WB: C2C12 cell lysate was probed with anti-Myosin Heavy Chain (MyHC) (1:1000 dilution).
05-716
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      Overview

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Reactividad según especies Aplicaciones clave Hospedador Formato Tipo de anticuerpo
      Conejo, Ser humano, Rata, Ratón, Pez cebra, DrWB, InmunohistoquímicaRatónAscitosAnticuerpo monoclonal
      Descripción
      Número de catálogo 05-716
      Marca de familia Upstate
      Sinónimos myosin, heavy polypeptide 1, skeletal muscle, adult Myosin heavy chain 1 Myosin heavy chain 2x Myosin heavy chain IIx/d Myosin heavy chain, skeletal muscle, adult 1 myosin, heavy chain 1, skeletal muscle, adult
      Nombre comercial
      • Upstate
      Descripción Anti-Myosin Heavy Chain Antibody, clone A4.1025
      Información preliminar Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.
      Referencias bibliográficas
      Información del producto
      Formato Ascitos
      Control
      • RIPA lysates from C2C12 cells
      Presentación Unpurified mouse ascites monoclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
      Aplicaciones
      Aplicación Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.
      Aplicaciones clave
      • Western Blotting
      • Inmunohistoquímica
      Notas de aplicación Immunohistochemistry:
      This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).
      Información biológica
      Especies inmunógenas Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.
      Clon A4.1025
      Concentración Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hospedador Ratón
      Especificidad This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.
      Isotipo IgG
      Reactividad según especies Conejo Ser humano Rata Ratón Pez cebra Drosophila
      Species Reactivity Note Human, mouse, rat, rabbit, zebrafish and Drosophila.
      Tipo de anticuerpo Anticuerpo monoclonal
      Número de Entrez Gene
      Resumen de Entrez Gene Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
      Símbolo de gen
      • MYHSA1
      • MYH1
      • MyHC-2X/D
      • MGC133384
      • MYHa
      • MyHC-IIx/d
      • MyHC-2x
      • Myosin-1
      Método de purificación Unpurified
      Número UniProt
      Resumen UniProt FUNCTION: SwissProt: P12882 # Muscle contraction.
      SIZE: 1939 amino acids; 223115 Da
      SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
      SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
      DOMAIN: SwissProt: P12882 The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
      SIMILARITY: Contains 1 IQ domain. & Contains 1 myosin head-like domain.
      MISCELLANEOUS: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
      Información fisicoquímica
      Dimensiones
      Información de materiales
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Aseguramiento de calidad Routinely evaluated by Western Blot on RIPA lysates from C2C12 cells.

      Western Blot Analysis:
      1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.
      Declaración de uso
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Información de almacenamiento y transporte
      Condiciones de almacenamiento Stable for 1 year at -20ºC from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Información sobre embalaje
      Cantitad 200 µL
      Información de transporte
      Información complementaria
      Especificaciones

      Documentation

      Certificados de análisis

      CargoNúmero de lote
      Anti-Myosin Heavy Chain (MyHC), clone A4.1025 (mouse ascites) - DAM1408805DAM1408805
      Anti-Myosin Heavy Chain, clone A4.10252472931
      Anti-Myosin Heavy Chain, clone A4.1025 - 07020515660702051566
      Anti-Myosin Heavy Chain, clone A4.1025 - 2451224512
      Anti-Myosin Heavy Chain, clone A4.1025 - 25006732500673
      Anti-Myosin Heavy Chain, clone A4.1025 - DAM1394773DAM1394773
      Anti-Myosin Heavy Chain, clone A4.1025 - JBC1361185JBC1361185

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Effects of doxorubicin cancer therapy on autophagy and the ubiquitin-proteasome system in long-term cultured adult rat cardiomyocytes.
      Polychronis Dimitrakis,Maria-Iris Romay-Ogando,Francesco Timolati,Thomas M Suter,Christian Zuppinger
      Cell and tissue research 350 2012

      Mostrar resumen
      22864983
      Comparison of various kinds of bone marrow stem cells for the repair of infarcted myocardium: single clonally purified non-hematopoietic mesenchymal stem cells serve as a superior source.
      Shaoheng Zhang, Junbo Ge, Aijun Sun, Danling Xu, Juying Qian, Jinyi Lin, Yan Zhao, Haifeng Hu, Ying Li, Keqiang Wang, Yunzeng Zou
      Journal of cellular biochemistry 99 1132-47 2006

      Mostrar resumen
      16795039
      Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development
      Maggs, A. M., et al
      J Muscle Res Cell Motil, 21:101-13 (2000) 2000

      Immunoblotting (Western), Immunohistochemistry (Tissue)10961835
      Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog
      Blagden, C. S., et al
      Genes Dev, 11:2163-75 (1997) 1997

      Immunohistochemistry (Tissue)9303533
      Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception
      Cho, M, et al
      J Cell Sci, 107 ( Pt 9):2361-71 (1994) 1994

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