70956 | Rosetta™(DE3)pLysS Competent Cells

70956
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      70956-3
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          0.4 ml
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          70956-4
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              1 ml
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              Description
              Overview Rosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLysS, the rare tRNA genes are present on the same plasmids that carries the T7 lysozyme gene.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

              pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
              Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE (CamR)
              Catalogue Number 70956
              Brand Family Novagen®
              References
              Product Information
              Components
              0.4 ml1 mlComponent
              2 × 0.2 ml5 × 0.2 mlRosetta(DE3)pLysS Competent Cells
              2 × 2 ml4 × 2 mlSOC Medium
              2 ng2 ngTest Plasmid
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage ≤ -70°C
              Licence Brookhaven This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.
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              70956

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              Title
              Competent Cell Brochure

              Citations

              Title
            • Marc Graille, Maxime Chaillet and Herman van Tilbeurgh. (2008) Structure of yeast Dom34: a protein related to translation termination factor eRF1 and involved in No-Go decay. Journal of Biological Chemistry 283, 7145-7154.
            • Jesús Prieto, et al. (2008) Generation and analysis of mesophilic variants of the thermostable archaeal I-DmoI homing endonuclease. Journal of Biological Chemistry 283, 4364-4374.
            • Sunish Kumar Radhakrishnan, Martin Thanbichler and Patrick H. Viollier. (2008) The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus. Genes and Development 22, 212-225.
            • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
            • Markus C. Gershater, Ian Cummins and Robert Edwards. (2007) Herbicide bioactivation in Arabidopsis thaliana: Role of a carboxylesterase. Journal of Biological Chemistry 282, 21460-21466.
            • Robert T. Glover, et al. (2007) The two-component regulatory system senX3-regX3 regulates phosphate-dependent gene expression in Mycobacterium smegmatis. Journal of Bacteriology 189, 5495-5503.
            • Anette Thysse Jonstrup, et al. (2007) The 1.4-Å crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme. Nucleic Acids Research 35, 3153-3164.
            • David K. Liscombe and Peter J. Facchini. (2007) Molecular cloning and characterization of tetrahydroprotoberberine cis-N-methyltransferase, an enzyme involved in alkaloid biosynthesis in opium poppy. Journal of Biological Chemistry 282, 14741-14751.
            • Erika J. Mancini, et al. (2007) Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 A resolution. Protein Science 16, 2294-2300.
            • Phillip J. Monaghan, David Leys and Nigel S. Scrutton. (2007) Mechanistic aspects and redox properties of hyperthermophilic L-proline dehydrogenase from Pyrococcus furiosus related to dimethylglycine dehydrogenase/oxidase. Federation of American Societies for Experimental Biology Journal 274, 2070–2087.
            • Xiuhua Pang and Susan T. Howard. (2007) Regulation of the α-crystallin gene acr2 by the mprAB two-component system of Mycobacterium tuberculosis. Journal of Bacteriology 189, 6213-6221.
            • Rachel Pinto, et al. (2007) Sulfite reduction in Mycobacteria. Journal of Bacteriology 189, 6714-6722.
            • Chantal Abergel, et al. (2006) Impact of the excision of an ancient repeat insertion on Rickettsia conorii guanylate kinase activity. Molecular Biology and Evolution 23, 2112-2122..
            • Gina Boanca, Aaron Sand and Joseph J. Barycki. (2006) Uncoupling enzymatic and autoprocessing activities of H. pylori γ-glutamyltranspeptidase. Journal of Biological Chemistry 281, 19029-19037.
            • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
            • Jinshui Fan, Yoshihiro Matsumoto and David M. Wilson, III. (2006) Nucleotide sequence and DNA secondary structure, as well as replication protein A, modulate the single-stranded abasic endonuclease activity of APE1. Journal of Biological Chemistry 281, 3889-3898.
            • Guillaume Gabant, et al. (2006) THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain. Nucleic Acids Research 34, 2483-2494.
            • Yannick Gueguen, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure, antimicrobial activities, and gene expression. Journal of Biological Chemistry 281, 313-323.
            • Antonio A. Iniesta, et al. (2006) A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression. Procedings of the National Academy of Science 103, 10935-10940.
            • John F. Langenheim, et al. (2006) Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. Molecular Endocrinology 20, 661-674.
            • Ekaterina Minskaia, et al. (2006) Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis. Proceedings of the National Academy of Sciences (USA) 103, 5108-5113.
            • Steven Raynard, Wendy Bussen and Patrick Sung. (2006) A double Holliday junction dissolvasome comprising BLM, topoisomerase III, and BLAP75. Journal of Biological Chemistry 281, 13861-13864.
            • Mieke F. Roelofs, et al. (2006) Identification of small heat shock protein B8 (HSP22) as a novel TLR4 ligand and potential involvement in the pathogenesis of rheumatoid arthritis. Journal of Immunology 176, 7021-7027.
            • Sharada Sivaraman and Jack F. Kirsch. (2006) The narrow substrate specificity of human tyrosine aminotransferase – the enzyme deficient in tyrosinemia type II. Federation of European Biochemical Societies Journal 273, 1920-1929.
            • M. Wang and K. A. Hudak. (2006) A novel interaction of pokeweed antiviral protein with translation initiation factors 4G and iso4G: a potential indirect mechanism to access viral RNAs. Nucleic Acids Research 34, 1174-1181.
            • Jean-Louis Baneres, et al. (2005) Molecular characterization of a purified 5-HT4 receptor. A structural basis for drug efficacy. Journal of Biological Chemistry 280, 20253-20260.
            • Jean-Marie Bourhis, et al. (2005) The intrinsically disordered C-terminal domain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via two distinct sites and remains predominantly unfolded. Protein Science 14, 1975-1992.
            • Neelam Desai, et al. (2005) Two steps in Maf1 dependent repression of transcription by RNA Polymerase III. Journal of Biological Chemistry 280, 6455-6462.
            • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
            • Jinshui Fan, Yoshihiro Matsumoto and David M. Wilson, III. (2006) Nucleotide sequence and DNA secondary structure, as well as replication protein A, modulate the single-stranded abasic endonuclease activity of APE1. Journal of Biological Chemistry 281, 3889-3898.
            • Guillaume Gabant, et al. (2006) THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain. Nucleic Acids Research 34, 2483-2494.
            • Yannick Gueguen, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure, antimicrobial activities, and gene expression. Journal of Biological Chemistry 281, 313-323.
            • Antonio A. Iniesta, et al. (2006) A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression. Procedings of the National Academy of Science 103, 10935-10940.
            • John F. Langenheim, et al. (2006) Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. Molecular Endocrinology 20, 661-674.
            • Ekaterina Minskaia, et al. (2006) Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis. Proceedings of the National Academy of Sciences (USA) 103, 5108-5113.
            • Steven Raynard, Wendy Bussen and Patrick Sung. (2006) A double Holliday junction dissolvasome comprising BLM, topoisomerase III, and BLAP75. Journal of Biological Chemistry 281, 13861-13864.
            • Mieke F. Roelofs, et al. (2006) Identification of small heat shock protein B8 (HSP22) as a novel TLR4 ligand and potential involvement in the pathogenesis of rheumatoid arthritis. Journal of Immunology 176, 7021-7027.
            • Sharada Sivaraman and Jack F. Kirsch. (2006) The narrow substrate specificity of human tyrosine aminotransferase – the enzyme deficient in tyrosinemia type II. Federation of European Biochemical Societies Journal 273, 1920-1929.
            • M. Wang and K. A. Hudak. (2006) A novel interaction of pokeweed antiviral protein with translation initiation factors 4G and iso4G: a potential indirect mechanism to access viral RNAs. Nucleic Acids Research 34, 1174-1181.
            • Jean-Louis Baneres, et al. (2005) Molecular characterization of a purified 5-HT4 receptor. A structural basis for drug efficacy. Journal of Biological Chemistry 280, 20253-20260.
            • Jean-Marie Bourhis, et al. (2005) The intrinsically disordered C-terminal domain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via two distinct sites and remains predominantly unfolded. Protein Science 14, 1975-1992.
            • Neelam Desai, et al. (2005) Two steps in Maf1 dependent repression of transcription by RNA Polymerase III. Journal of Biological Chemistry 280, 6455-6462.
            • Aimee L. Eggler, et al. (2005) Modifying specific cysteines of the electrophile-sensing human Keap1 protein is insufficient to disrupt binding to the Nrf2 domain Neh2. Proceedings of the National Academy of Sciences (USA) 102, 10070-10075.
            • Matthias Grimmler, et al. (2005) Unrip, a factor implicated in cap-independent translation, associates with the cytosolic SMN complex and influences its intracellular localization. Human Molecular Genetics 14, 3099-3111.
            • George A. Kassavetis, et al. (2005) Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIB. Proceedings of the National Academy of Sciences (USA) 102, 15406-15411.
            • Sunil Laxman, Ana Rascon and Joseph A. Beavo. (2005) Trypanosome cyclic nucleotide phosphodiesterase 2B binds cAMP through its GAF-A domain. Journal of Biological Chemistry 280, 3771-3779.
            • Xiaobing Lu, et al. (2005) Membrane fusion induced by neuronal SNAREs transits through hemifusion. Journal of Biological Chemistry 280, 30538-30541.
            • Woo-Hyun Park, et al. (2005) Direct DNA binding activity of the fanconi anemia D2 protein. Journal of Biological Chemistry 280, 23593-23598.
            • Oliver Schilling, et al. (2005) Exosite modules guide substrate recognition in the ZiPD/ElaC protein family. Journal of Biological Chemistry 280, 17857-17862.
            • Christian Siebold, et al. (2005) High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multidomain flavoenzyme-signaling molecule. Proceedings of the National Academy of Sciences (USA) 102, 16836-16841.
            • Detlef Vullhorst and Andres Buonanno. (2005) Multiple GTF2I- like repeats of general transcription factor 3 exhibit DNA binding properties: Evidence for a common origin as a sequence-specific DNA interaction module. Journal of Biological Chemistry 280, 31722-31731.
            • Hua Wei, et al. (2005) Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase. Journal of Biological Chemistry 280, 37041-37047.
            • Shin-ichiro Yoshimura, et al. (2005) Convergence of cell cycle regulation and growth factor signals on GRASP65. Journal of Biological Chemistry 280, 23048-23056.
            • Yinghui Zhang, et al. (2005) A partially zipped SNARE complex stabilized by the membrane. Journal of Biological Chemistry 280, 15595-15600.
            • Roger B. Dodd, et al. (2004) Solution structure of the Kaposi's sarcoma-associated herpesvirus K3 N-terminal domain reveals a Novel E2-binding C4HC3-type RING. Journal of Biological Chemistry 279, 53840-53847.
            • Nicole LaRonde-LeBlanc and Alexander Wlodawer. (2004) Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases. Structure 12, 1585-1594.
            • Liliya A. Yatsunyk, Benjamin E. Ramirez and Amy C. Rosenzweig. (2004) Yeast Cox17 solution structure and copper(I) binding. Journal of Biological Chemistry 279, 53584-53592.
            • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
            • Jinshui Fan, Yoshihiro Matsumoto and David M. Wilson, III. (2006) Nucleotide sequence and DNA secondary structure, as well as replication protein A, modulate the single-stranded abasic endonuclease activity of APE1. Journal of Biological Chemistry 281, 3889-3898.
            • Guillaume Gabant, et al. (2006) THUMP from archaeal tRNA:m22G10 methyltransferase, a genuine autonomously folding domain. Nucleic Acids Research 34, 2483-2494.
            • Yannick Gueguen, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure, antimicrobial activities, and gene expression. Journal of Biological Chemistry 281, 313-323.
            • Antonio A. Iniesta, et al. (2006) A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression. Procedings of the National Academy of Science 103, 10935-10940.
            • John F. Langenheim, et al. (2006) Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. Molecular Endocrinology 20, 661-674.
            • Ekaterina Minskaia, et al. (2006) Discovery of an RNA virus 3'->5' exoribonuclease that is critically involved in coronavirus RNA synthesis. Proceedings of the National Academy of Sciences (USA) 103, 5108-5113.
            • Steven Raynard, Wendy Bussen and Patrick Sung. (2006) A double Holliday junction dissolvasome comprising BLM, topoisomerase III, and BLAP75. Journal of Biological Chemistry 281, 13861-13864.
            • Mieke F. Roelofs, et al. (2006) Identification of small heat shock protein B8 (HSP22) as a novel TLR4 ligand and potential involvement in the pathogenesis of rheumatoid arthritis. Journal of Immunology 176, 7021-7027.
            • Sharada Sivaraman and Jack F. Kirsch. (2006) The narrow substrate specificity of human tyrosine aminotransferase – the enzyme deficient in tyrosinemia type II. Federation of European Biochemical Societies Journal 273, 1920-1929.
            • M. Wang and K. A. Hudak. (2006) A novel interaction of pokeweed antiviral protein with translation initiation factors 4G and iso4G: a potential indirect mechanism to access viral RNAs. Nucleic Acids Research 34, 1174-1181.
            • Jean-Louis Baneres, et al. (2005) Molecular characterization of a purified 5-HT4 receptor. A structural basis for drug efficacy. Journal of Biological Chemistry 280, 20253-20260.
            • Jean-Marie Bourhis, et al. (2005) The intrinsically disordered C-terminal domain of the measles virus nucleoprotein interacts with the C-terminal domain of the phosphoprotein via two distinct sites and remains predominantly unfolded. Protein Science 14, 1975-1992.
            • Neelam Desai, et al. (2005) Two steps in Maf1 dependent repression of transcription by RNA Polymerase III. Journal of Biological Chemistry 280, 6455-6462.
            • Aimee L. Eggler, et al. (2005) Modifying specific cysteines of the electrophile-sensing human Keap1 protein is insufficient to disrupt binding to the Nrf2 domain Neh2. Proceedings of the National Academy of Sciences (USA) 102, 10070-10075.
            • Matthias Grimmler, et al. (2005) Unrip, a factor implicated in cap-independent translation, associates with the cytosolic SMN complex and influences its intracellular localization. Human Molecular Genetics 14, 3099-3111.
            • George A. Kassavetis, et al. (2005) Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIB. Proceedings of the National Academy of Sciences (USA) 102, 15406-15411.
            • Sunil Laxman, Ana Rascon and Joseph A. Beavo. (2005) Trypanosome cyclic nucleotide phosphodiesterase 2B binds cAMP through its GAF-A domain. Journal of Biological Chemistry 280, 3771-3779.
            • Xiaobing Lu, et al. (2005) Membrane fusion induced by neuronal SNAREs transits through hemifusion. Journal of Biological Chemistry 280, 30538-30541.
            • Woo-Hyun Park, et al. (2005) Direct DNA binding activity of the fanconi anemia D2 protein. Journal of Biological Chemistry 280, 23593-23598.
            • Oliver Schilling, et al. (2005) Exosite modules guide substrate recognition in the ZiPD/ElaC protein family. Journal of Biological Chemistry 280, 17857-17862.
            • Christian Siebold, et al. (2005) High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multidomain flavoenzyme-signaling molecule. Proceedings of the National Academy of Sciences (USA) 102, 16836-16841.
            • Detlef Vullhorst and Andres Buonanno. (2005) Multiple GTF2I- like repeats of general transcription factor 3 exhibit DNA binding properties: Evidence for a common origin as a sequence-specific DNA interaction module. Journal of Biological Chemistry 280, 31722-31731.
            • Hua Wei, et al. (2005) Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase. Journal of Biological Chemistry 280, 37041-37047.
            • Shin-ichiro Yoshimura, et al. (2005) Convergence of cell cycle regulation and growth factor signals on GRASP65. Journal of Biological Chemistry 280, 23048-23056.
            • Yinghui Zhang, et al. (2005) A partially zipped SNARE complex stabilized by the membrane. Journal of Biological Chemistry 280, 15595-15600.
            • Roger B. Dodd, et al. (2004) Solution structure of the Kaposi's sarcoma-associated herpesvirus K3 N-terminal domain reveals a Novel E2-binding C4HC3-type RING. Journal of Biological Chemistry 279, 53840-53847.
            • Nicole LaRonde-LeBlanc and Alexander Wlodawer. (2004) Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases. Structure 12, 1585-1594.
            • Liliya A. Yatsunyk, Benjamin E. Ramirez and Amy C. Rosenzweig. (2004) Yeast Cox17 solution structure and copper(I) binding. Journal of Biological Chemistry 279, 53584-53592.
            • Qin Zhao, et al. (2004) Production in two-liter beverage bottles of protins for NMR structur
            • User Protocols

              Title
              TB009 Competent Cells
              TB053 Academic and Non-profit Laboratory Assurance Letter

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