70773 | pET Expression System 26b

70773
Price could not be retrieved
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability Limited Availability
Discontinued
There is a newer version of this product
Limited Quantities Available
Availability to be confirmed
      Will advise
      View Pricing & Availability
         CompareClick To Print This Page

      Overview

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      70773-3
      Retrieving availability...
      Limited Availability Limited Availability
      Discontinued
      There is a newer version of this product
      Limited Quantities Available
      Availability to be confirmed
          Will advise

          1 ea
          Retrieving price...
          Price could not be retrieved
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          Overview pET Expression Systems and pET
          pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

          Components: pET Expression Systems
          Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
          • 10 µg pET vector DNA (for each indicated plasmid)
          • 0.2 ml BL21 Glycerol Stock
          • 0.2 ml BL21(DE3) Glycerol Stock
          • 0.2 ml BL21(DE3)pLysS Glycerol Stock
          • 0.2 ml Induction Control Glycerol Stock

          Components: pET Expression Systems plus Competent Cells
          pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
          • 0.2 ml NovaBlue Competent Cells
          • 0.2 ml BL21(DE3) Competent Cells
          • 0.2 ml BL21(DE3)pLysS Competent Cells
          • 2 × 0.2 ml SOC Medium
          • 10 µl Test Plasmid

          These components are sufficient for up to 10 transformations in each host.

          Purification and Detection Reagents
          Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

          pET Expression System 26b
          The pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB071VM.




          The pET System allows for expression of target genes under control of the T7 promoter and is covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs. Commercial entities need to obtain a license from Brookhaven National Labs prior to use of this Product. Ccommercial use of this Product also requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          Catalogue Number 70773
          Brand Family Novagen®
          References
          Product Information
          Components
          Fusion tag His•Tag, HSV•Tag
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze No
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

          Languages
          Danmark Deutschland Ellas
          English España France
          Italia Korea Magyarország
          Nederland  Norge Polska
          Portugal Suomi Sverige
          България

          Certificates of Analysis

          TitleLot Number
          70773

          Citations

          Title
        • Wu. L., et al. (2009) Structural Basis for Proteolytic Specificity of the Human Apoptosis-Inducing Granzyme M J. Immunol. 183, 421.
        • Damien Bessière, et al. (2008) Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways. Journal of Biological Chemistry 283, 4352-4363.
        • Nobutaka Funa, Takayoshi Awakawa and Sueharu Horinouchi. (2007) Pentaketide resorcylic acid synthesis by Type III polyketide synthase from Neurospora crassa. Journal of Biological Chemistry 282, 14476-14481.
        • Yongchao Li, Diana C. Irwin and David B. Wilson. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Applied and Enviornmental Microbiology 73, 3165-3172.
        • Jean-Michel Heraud, et al. (2006) Subunit recombinant vaccine protects against monkeypox. Journal of immunology 177, 2552-2564.
        • Stan J. J. Brouns, et al. (2005) Engineering a selectable marker for hyperthermophiles. Journal of Biological Chemistry 280, 11422-11431.
        • Nobutaka Funa, et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. Journal of Biological Chemistry 280, 14514-14523.
        • Ryohei Ishii, et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. Journal of Biological Chemistry 280, 14138-14144.
        • Hongmin Li, et al. (2005) Crystal structures of T cell receptor β chains related to rheumatoid arthritis. Protein Science 14, 3025-3038.
        • Taishi Tsubouchi, et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Journal of Biological Chemistry 280, 18511-18516.
        • Florence Vincent, Gideon J. Davies and James A. Brannigan. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? Journal of Biological Chemistry 280, 19649-19655.
        • Yu-hang Chen, et al. (2004) Structural basis of the α1-β subunit interaction of voltage-gated Ca2+ channels. 429, 675-680.
        • Watchrra Teartasin, et al. (2004) Expression, purification, and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallographica D60, 1137-1138.
        • Shalom D. Goldberg, et al. (2003) Identification of residues critical for catalysis in a class C b-lactamase by combinatorial scanning mutagenesis. Protein Science 12, 1633-1645.
        • User Protocols

          Title
          TB053 Academic and Non-profit Laboratory Assurance Letter
          TB055 pET System Manual

          Vector Map

          Title
          TB071VM pET-26b(+) Vector Map

          Related Products & Applications

          Related Products

           Catalogue Number Description  
          70778 pET Expression System 28 plus Competent Cells Show Pricing & Availability
          70779 pET Expression System 29 Show Pricing & Availability
          70780 pET Expression System 29 plus Competent Cells Show Pricing & Availability
          70781 pET Expression System 30 Show Pricing & Availability
          70782 pET Expression System 30 plus Competent Cells Show Pricing & Availability
          69405 pET Expression System 11 Show Pricing & Availability
          69406 pET Expression System 11 plus Competent Cells Show Pricing & Availability
          69409 pET Expression System 3 Show Pricing & Availability
          69410 pET Expression System 3 plus Competent Cells Show Pricing & Availability
          69415 pET Expression System 9 Show Pricing & Availability
          69416 pET Expression System 9 plus Competent Cells Show Pricing & Availability
          69726 pET System 17b Show Pricing & Availability
          69731 pET System 17b plus Competent Cells Show Pricing & Availability
          70753 pET Expression System 14b Show Pricing & Availability
          70754 pET Expression System 14b plus Competent Cells Show Pricing & Availability
          70755 pET Expression System 15b Show Pricing & Availability
          70756 pET Expression System 15b plus Competent Cells Show Pricing & Availability
          70757 pET Expression System 16b Show Pricing & Availability
          70758 pET Expression System 16b plus Competent Cells Show Pricing & Availability
          70759 pET Expression System 19b Show Pricing & Availability
          70760 pET Expression System 19b plus Competent Cells Show Pricing & Availability
          70761 pET Expression System 20b Show Pricing & Availability
          70762 pET Expression System 20b plus Competent Cells Show Pricing & Availability
          70763 pET Expression System 21 Show Pricing & Availability
          70764 pET Expression System 21 plus Competent Cells Show Pricing & Availability
          70765 pET Expression System 22b Show Pricing & Availability
          70766 pET Expression System 22b plus Competent Cells Show Pricing & Availability
          70767 pET Expression System 23 Show Pricing & Availability
          70768 pET Expression System 23 plus Competent Cells Show Pricing & Availability
          70769 pET Expression System 24 Show Pricing & Availability
          70770 pET Expression System 24 plus Competent Cells Show Pricing & Availability
          70771 pET Expression System 25b Show Pricing & Availability
          70772 pET Expression System 25b plus Competent Cells Show Pricing & Availability
          70774 pET Expression System 26b plus Competent Cells Show Pricing & Availability
          70775 pET Expression System 27b Show Pricing & Availability
          70776 pET Expression System 27b plus Competent Cells Show Pricing & Availability
          70777 pET Expression System 28 Show Pricing & Availability

          Related Products By: Application Facette

          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors