70765 | pET Expression System 22b

70765
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      Overview

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          Description
          Overview pET Expression Systems and pET
          pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

          Components: pET Expression Systems
          Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
          • 10 µg pET vector DNA (for each indicated plasmid)
          • 0.2 ml BL21 Glycerol Stock
          • 0.2 ml BL21(DE3) Glycerol Stock
          • 0.2 ml BL21(DE3)pLysS Glycerol Stock
          • 0.2 ml Induction Control Glycerol Stock

          Components: pET Expression Systems plus Competent Cells
          pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
          • 0.2 ml NovaBlue Competent Cells
          • 0.2 ml BL21(DE3) Competent Cells
          • 0.2 ml BL21(DE3)pLysS Competent Cells
          • 2 × 0.2 ml SOC Medium
          • 10 µl Test Plasmid

          These components are sufficient for up to 10 transformations in each host.

          Purification and Detection Reagents
          Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

          pET Expression System 22b
          The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB038VM.




          The pET System allows for expression of target genes under control of the T7 promoter and is covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs. Commercial entities need to obtain a license from Brookhaven National Labs prior to use of this Product. Ccommercial use of this Product also requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          Catalogue Number 70765
          Brand Family Novagen®
          References
          Product Information
          Components
          10 µgpET vector DNA(s)
          0.2 mlHost bacterial strains BL21, BL21(DE3), and BL21(DE3)pLysS, glycerol stocks
          0.2 mlInduction control clone, glycerol stock
          Fusion tag His•Tag
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze No
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

          Languages
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          Certificates of Analysis

          TitleLot Number
          70765

          Citations

          Title
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        • Henri-Pierre Fierobe, et al. (2005) Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined tri-functional scaffoldin. Journal of Biological Chemistry 280, 16325-16334.
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        • Isabelle S. Lucet, et al. (2005) Identification of the structural and functional domains of the large serine recombinase tnpX from Clostridium perfringens. Journal of Biological Chemistry 280, 2503-2511.
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        • Kyoung Joon Oh, et al. (2005) Conformational changes in BID, a pro-apoptotic BCL-2 family member, upon membrane binding: a site-directed spin labeling study. Journal of Biological Chemistry 280, 753-767.
        • Laura M. Roy, et al. (2007) Human polymorphic variants of the NEIL1 DNA glycosylase. Journal of Biological Chemistry 282, 15790-15798.
        • Katja Strube, Simon de Vries and Rainer Cramm. (2007) Formation of a dinitrosyl iron complex by NorA, a nitric oxide binding di-iron protein from Ralstonia eutropha H16. Journal of Biological Chemistry 282, 20292-20300.
        • Elizebeth C. Turner and Charles J. Dorman. (2007) H-NS antagonism in Shigella flexneri by VirB, a virulence gene yranscription regulator that is closely related to plasmid partition factors. Journal of Bacteriology 189, 3403-3413.
        • Ernest P. Williams, et al. (2007) Mycobacterium tuberculosis SigF regulates genes encoding cell wall-associated proteins and directly regulates the transcriptional regulatory gene phoY1. Journal of Bacteriology 189, 4234-4242.
        • Frédéric Chédin, et al. (2006) The AddAB helicase/nuclease forms a stable complex with its cognate sequence during translocation. Journal of Biological Chemistry 281, 18610-18617.
        • John F. Langenheim, et al. (2006) Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. Molecular Endocrinology 20, 661-674.
        • Emma Carmelo, et al. (2005) The unstructured N-terminal tail of ParG modulates assembly of a quaternary nucleoprotein complex in transcription repression. Journal of Biological Chemistry 280, 28683-28691.
        • Veronika Csizmok, et al. (2005) A novel 2D electrophoresis technique for the identification of intrinsically unstructured proteins. Molecular and Cellular Proteomics 5, 265-273.
        • Natalie Elia, et al. (2005) Excess of Gβe over Gqαe in vivo prevents dark, spontaneous activity of Drosophila photoreceptors. Journal of Cell Biology 171, 517-526.
        • Henri-Pierre Fierobe, et al. (2005) Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined tri-functional scaffoldin. Journal of Biological Chemistry 280, 16325-16334.
        • Laurent Loiseau, et al. (2005) Analysis of the heteromeric CsdA-CsdE cysteine desulfurase, assisting Fe-S cluster biogenesis in Escherichia coli. Journal of Biological Chemistry 280, 26760-26769.
        • Isabelle S. Lucet, et al. (2005) Identification of the structural and functional domains of the large serine recombinase tnpX from Clostridium perfringens. Journal of Biological Chemistry 280, 2503-2511.
        • Jeremy Moore, et al. (2005) Recognition of saccharides by the OpcA, OpaD and OpaB outer membrane proteins from Neisseria meningitidis. Journal of Biological Chemistry 280, 31489-31497.
        • Kyoung Joon Oh, et al. (2005) Conformational changes in BID, a pro-apoptotic BCL-2 family member, upon membrane binding: a site-directed spin labeling study. Journal of Biological Chemistry 280, 753-767.
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        • Guoqiang Zhu, et al. (2005) Histone H1 proteins act as receptors for the 987P fimbriae of enterotoxigenic Escherichia coli. Journal of Biological Chemistry 280, 23057-23065.
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        • User Protocols

          Title
          TB053 Academic and Non-profit Laboratory Assurance Letter
          TB055 pET System Manual

          Vector Map

          Title
          TB038VM pET-22b(+) Vector Map

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