70181 | NovaBlue Singles™ Competent Cells

70181
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      Overview

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      70181-3
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      Discontinued
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          11 rxn
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          70181-4
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              22 rxn
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              Description
              Overview

              NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-µl volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance).

              Genotype:
              endA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)
              Catalogue Number 70181
              Brand Family Novagen®
              References
              Product Information
              Components
              11 or 22Singles Competent Cells
              2 or 4 × 2 mlSOC Medium
              10 µlTest Plasmid
              Genotype endA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA+B+ lacIqZΔM15::Tn10] (TetR)
              Guaranteed efficiency Guaranteed efficiency: > 1.5 x 10⁸ cfu/µg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              MSDS

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              Certificates of Analysis

              TitleLot Number
              70181

              Brochure

              Title
              The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

              Citations

              Title
            • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
            • Thomas S. Lendvay, et al. (2007) Compensatory paracrine mechanisms that define the urothelial response to injury in partial bladder outlet obstruction. American Journal of Physiology: Renal 293, F1147-F1156.
            • Alvaro San Millan, et al. (2007) β-Lactam resistance in Haemophilus parasuis is mediated by plasmid pB1000 bearing blaROB-1. Antimicrobial Agents and Chemotherapy 51, 2260-2264.
            • Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.
            • Pernilla Larsson Birgander, et al. (2005) Nucleotide-dependent formation of catalytically competent dimers from engineered monomeric ribonucleotide reductase protein R1. Journal of Biological Chemistry 280, 14997-15003.
            • Anna B. Gilg, et al. (2005) Isolation and functional expression of an animal geranyl diphosphate synthase and its role in bark beetle pheromone biosynthesis. Proceedings of the National Academy of Sciences (USA) 102, 9760-9765.
            • Miho Hirabayashi, et al. (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146, 399-405.
            • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
            • Catherine Paradis-Bleau, Francois Sanschagrin and Roger C. Levesque. (2005) Peptide inhibitors of the essential cell division protein FtsA. Protein Engineering Design and Selection 18, 85-91.
            • Francois Sanschagrin and Roger C. Levesque. (2005) A specific peptide inhibitor of the class B metallo-{beta}-lactamase L-1 from Stenotrophomonas maltophilia identified using phage display. Journal of Antimicrobial Chemotherapy 55, 252-255.
            • M. Shimizu, et al. (2005) Salmon serum 22 kDa insulin-like growth factor-binding protein (IGFBP) is IGFBP-1. Journal of Endocrinology 184, 267-276.
            • Marko Tammenkoski, et al. (2005) An unusual, his-dependent family I pyrophosphatase from Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 41819-41826.
            • Eric A. Grovender, et al. (2004) Single-chain antibody fragment-based Adsorbent for the extracorporeal removal of β2-microglobulin. Kidney International 65, 310-322.
            • Laura I. Katona, et al. (2004) The Fur homologue in Borrelia burgdorferi. Journal of Bacteriology 186, 6443-6456.
            • Sharmila Sivendran, et al. (2004) Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. Journal of Biological Chemistry 279, 53789-53797.
            • Deanne M. Compaan and W. Ross Ellington. (2003) Functional consequences of a gene duplication and fusion event in an arginine kinase. 206, 1545-1556.
            • Xinxin Gao, et al. (2003) Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences. Nucleic Acids Research 31, e143-.
            • Yusuf Kaya and James Ofengand. (2003) A novel unanticipated type of pseudouridine synthase with homologs in bacteria, archaea, and eukarya. RNA 9, 711-721.
            • Michael B. Murphy, et al. (2003) An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Research 31, e110-.
            • Céline Pujol and James B. Bliska. (2003) The ability to replicate in macrophages Is conserved between Yersinia pestis and Yersinia pseudotuberculosis. Infection and Immunity 71, 5892–5899.
            • User Protocols

              Title
              TB009 Competent Cells

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              Categories

              Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Competent Cells for Cloning