71510 | D-Tube™ Dialyzer Maxi, MWCO 12-14 kDa

71510
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      71510-3
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          Description
          Overview
          D-Tube™ Dialyzers Mini, Midi, Maxi, and Mega
          The D-Tube™ Dialyzers be used for dialysis and electroelution of proteins, RNA, DNA, and oligonucleotides from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuge, or laborious steps to manipulate small sample volumes. The sample is added and removed using a standard laboratory pipet. Available with molecular weight cutoffs (MWCO) from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in four volume capacities: mini (10-250 µl), midi (50-800 micro;l), maxi (100-3000 µl), and mega (10ml, 15ml, and 20 ml). The membrane is ultra-clean, EDTA-treated regenerated cellulose, sulfur- and heavy metal-free.

          The D-Tube Dialyzers Mini, Midi, and Maxi contain 10 D-Tube Dialyzers and one floating rack that can hold up to four D-Tube Dialyzers. The D-Tube Dialyzers Mega contain 10 or 50 tubes and one or 5 floating racks to hold one D-Tube Mega in the exchange buffer.

          D-Tube™ Electroelution Accessory Kit
          The D-Tube Electroelution Accessory Kit provides a support tray for the D-Tube Dialyzers (compatible with most commercially-available horizontal electrophoresis units) and optimized reagents for protein and nucleic acid precipitation following electroelution. The kit also contains an optimized buffer for the removal of SDS from extracted protein samples, making them compatible with MALDI-MS analysis. The combination of D-Tube Dialyzers and D-Tube Electroelution Accessory Kit provides a unique tool for extraction of any protein, protein-protein and protein-DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels with 60% recovery yield in less than 2 hours. The procedure efficiently recovers proteins and simultaneously removes ampholytes from proteins run on 2D gels. Extracted proteins are compatible with most downstream applications such as MALDIMS, animal immunization for antibody production, HPLC analysis, peptide mapping, and functional assays. In addition, D-Tube Dialyzers can be used for oligonucleotides, RNA and DNA extraction from both polyacrylamide and agarose gels. Efficient extraction (>90%) is achieved for 15 nt oligos and for up to 80 kbp DNA fragments.

          Catalogue Number 71510
          Brand Family Novagen®
          Features and benefits
          • Easy-to-handle dialyzers for buffer exchange and removal of urea, detergents
          • Ideal for electroelution of proteins, protein-DNA complexes, oligonucleotides, DNA, and RNA from polyacrylamide and agarose gels
          • One-step procedure that does not require syringes or any special equipment
          • Sample volume recovery > 97%
          • Protease, RNAse, DNAse, and PCR product free
          References
          Product Information
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          Safety Information according to GHS
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          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice
          Toxicity Standard Handling
          Storage +15°C to +30°C
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          Certificates of Analysis

          TitleLot Number
          71510

          Brochure

          Title
          Dialysis & Filtration Product Guide
          Fast and Easy Sample Dialysis. When downstream quality matters, make sure your upstream tools are the best.

          Citations

          Title
        • Job E. Lopez, et al. (2007) Immunogenicity of Anaplasma marginale type IV secretion system proteins in a protective outer membrane vaccine. Infection and Immunity 75, 2333-2342.
        • User Protocols

          Title
          TB422 D-Tube™ Dialyzers: Mini, Midi, Maxi, and D-Tube96™

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          Categories

          Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Protein Dialyzers