SCR055 | ENStem Human Neural Progenitor Expansion Kit

SCR055
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      Overview

      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue Number SCR055
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description ENStem Human Neural Progenitor Expansion Kit
      Overview The ENStem-A Human Neural progenitor Expansion Kit contains ENStem-A Human Neural Progenitors and optimized Expansion medium. ENStem-A Human Neural Progenitor Cells are derived from NIH approved H9 human embryonic stem cells (hESCs). Using a proprietary method developed by Aruna Biomedical, these hESC derived neural progenitors proliferate as an adherent cell monolayer and can readily differentiate into different neuronal subtypes. Detailed spectral analyses indicate ENStem-A cells retained a normal diploid karyotype in culture after ten passages. ENStem-ATM human neural progenitor cells may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-A Human Neural Progenitor Cells.
      References
      Product Information
      Components Basic FGF, lyophilized, 10 ug ENSTem-A Human Neural Progenitors, 1 x 106 cells ENStem-A Neural Expansion Media, SCM004, 500 mls
      Applications
      Application The ENStem-A Human Neural progenitor Expansion Kit contains ENStem-A Human Neural Progenitors & optimized Expansion medium.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Species Reactivity Human
      Cell Line Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Each lot of ENStem-A Human Neural Progenitor Cells has been validated for high levels of expression of Nestin and Sox 2, and low level expression of Oct-4. The ability of ENStem-A cells to differentiate into multiple neuronal phenotypes and maintain a normal karyotype after multiple passages has been verified (please refer to datasheet figures). The cells have been confirmed to be negative for mycoplasma.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions EnStem Human Neural Progenitor Cells: When stored at the recommended storage conditions (liquid nitrogen), ENStem-A cells are stable up to five years. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages. ENStem-A Human Neural Expansion media ENStem-A Neural Expansion Medium should be stored at -20C until ready to use. Upon thawing, the expansion medium should be stored at 2-8C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.
      Packaging Information
      Material Size 1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      Contralaterally transplanted human embryonic stem cell-derived neural precursor cells (ENStem-A) migrate and improve brain functions in stroke-damaged rats.
      Chang, Da-Jeong, et al.
      Exp. Mol. Med., 45: e53 (2013) 2013

      Show Abstract
      24232252
      A continuum of cell states spans pluripotency and lineage commitment in human embryonic stem cells.
      Shelley R Hough,Andrew L Laslett,Sean B Grimmond,Gabriel Kolle,Martin F Pera
      PloS one 4 2009

      Show Abstract Full Text Article
      19890402

      Brochure

      Title
      Human Stem Cell Systems
      Stem Cellutions Newsletter: 2007, Volume 1

      Technical Info

      Title
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line
      ENStem-A Adherent Human Neural Progenitors: A New Source of Primary Human Neural Cells

      Data Sheet

      Title
      ENStem-A™ Human Neural Progenitor Expansion Kit - Data Sheet

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Stem Cell Lines