SCR051 | Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit

SCR051
10 isolations/kit  
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      Overview

      Key Spec Table

      Key Applications
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      Description
      Catalogue Number SCR051
      Description Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit
      Overview The Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit provides an improved methodology based on sequential grinding and enzymatic digestion of murine bone tissue for harvesting higher numbers of BM cells and HSC from the endosteal and central marrow regions.
      Background Information Hematopoietic stem cells (HSC) are frequently located in close proximity to bone lining cells within the endosteum. Recent studies demonstrated the significance of the endosteal region for hematopoiesis, and in particular that it contains a high number of HSCs. Consequently, traditional methods for harvesting bone marrow (BM) from mice via flushing of the long bones do not maximize the recovery of all HSCs. The Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit permits the optimal recovery of total cells and HSCs from mouse bones, and uniquely the isolation of HSCs specifically from the endosteal region. A combination of mechanical fragmentation by grinding of bones and enzymatic digestion is used for this purpose. Using this method, HSCs isolated specifically from the endosteum have been demonstrated to have a greater hematopoietic potential than HSCs isolated from the central marrow core including a 1.8-fold greater proliferative potential, an almost 2-fold greater ability to home to the bone marrow following tail vein injection and a significantly greater long-term hematopoietic reconstitution potential (Haylock, D. et al. Stem Cells, 2007). Additionally, this method has been shown to produce a 6-fold greater recovery of primitive HSCs (Lineage- Sca-1+ and c-Kit+) than traditional flushing methods.
      References
      Product Information
      Components
      • 4. Collagenase I - Ten (10) single use vials of 30 mg powdered enzyme
      • Sufficient reagents are supplied in the kit for 10 separate isolations.
      • 1. Mortar and pestle - One (1) porcelain mortar and pestle
      • 2. Cell Strainer - Twenty (20) cell strainers with 40 µm nylon mesh and blue polypropylene frame
      • 3. Dispase II - Ten (10) single use vials of 40 mg powdered enzyme
      Applications
      Application The Bone Marrow Harvesting & Hematopoietic Stem Cell Isolation Kit permits the optimal recovery of total cells & HSCs from mouse bones & uniquely the isolation of HSCs specifically from the endosteal region.
      Key Applications
      • Cell Harvesting
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Storage Conditions Mortar, pestle, and cell strainers can be stored at room temperature.
      Single use vials containing the enzymes Dispase II and Collagenase I should be stored at 2° to 8°C until ready to use.
      Packaging Information
      Material Size 10 isolations/kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      English

      References

      Reference overviewPub Med ID
      Granulocyte colony stimulating factor expands hematopoietic stem cells within the central but not endosteal bone marrow region.
      Jochen Grassinger,Brenda Williams,Gemma H Olsen,David N Haylock,Susan K Nilsson
      Cytokine 58 2012

      Show Abstract
      22341722
      Phenotypically identical hemopoietic stem cells isolated from different regions of bone marrow have different biologic potential.
      Jochen Grassinger,David N Haylock,Brenda Williams,Gemma H Olsen,Susan K Nilsson
      Blood 116 2010

      Show Abstract
      20631378
      Glucagon supports postabsorptive plasma glucose concentrations in humans with biologically optimal insulin levels.
      Cooperberg BA, Cryer PE
      Diabetes 59 2941-4. Epub 2010 Aug 10. 2010

      Show Abstract Full Text Article
      20699417
      Investigating the interactions between haemopoietic stem cells and their niche: methods for the analysis of stem cell homing and distribution within the marrow following transplantation.
      Brenda Williams,Susan K Nilsson
      Methods in molecular biology (Clifton, N.J.) 482 2009

      Show Abstract
      19089351

      Brochure

      Title
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 2
      Cellutions Newsletter: 2008, Volume 3

      Technical Info

      Title
      Maximal Recovery of Hematopoietic Stem Cells from Bone Marrow Endosteum

      User Guides

      Title
      Bone Marrow Harvesting & Hematopoietic Stem Cell Isolation Kit