S7821 | CpGenome Universal Methylated DNA

S7821
10 µg  
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      Overview

      Description
      Catalogue Number S7821
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      • CpGenome
      Description CpGenome Universal Methylated DNA
      Overview CpGenome™ Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.

      Materials Provided:

      Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.

      Validation:

      Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ™ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.

      CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ™ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
      References
      Product Information
      Presentation Liquid in TE (10mM Tris-HCL, 0.1mM EDTA) with no preservatives.
      Applications
      Application CpGenome Universal Methylated DNA is enzymatically methylated human male genomic DNA. It can be used as a methylation-positive control for gene methylation studies.
      Application Notes For MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Species Reactivity Human
      Modifications
      • Methylation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Recommended Storage: -15°C to -25°C, in aliquots, protected from freeze-thaws for up to two years from date of receipt. Do not freeze and thaw the same -20°C aliquot more than 3X for best results. Multiple freeze thaws can fragment DNA. Storage at -70°C can be used for longer term storage if necessary; minimize multiple freeze thaws for best results.
      Packaging Information
      Material Size 10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      English

      Certificates of Analysis

      TitleLot Number
      CpGenome Universal Methylated DNA - 2121935 2121935
      CpGenome Universal Methylated DNA - 2025131 2025131
      CpGenome Universal Methylated DNA - 2042210 2042210
      CpGenome Universal Methylated DNA - 2089504 2089504
      CpGenome Universal Methylated DNA - 2201579 2201579
      CpGenome™ Universal Methylated DNA -2475629 2475629
      CpGenome™ Universal Methylated DNA - 2328323 2328323
      CpGenome™ Universal Methylated DNA - 2395649 2395649
      CpGenome™ Universal Methylated DNA - 2430319 2430319
      CpGenome™ Universal Methylated DNA - 2276316 2276316

      References

      Reference overviewApplicationPub Med ID
      DNA hypermethylation and histone modifications downregulate the candidate tumor suppressor gene RRP22 on 22q12 in human gliomas.
      Natalie Schmidt,Sonja Windmann,Guido Reifenberger,Markus J Riemenschneider
      Brain pathology (Zurich, Switzerland) 22 2012

      Show Abstract
      21631628
      IDH1 and IDH2 mutations, immunohistochemistry and associations in a series of brain tumors.
      Mellai, Marta, et al.
      J. Neurooncol., 105: 345-57 (2011) 2011

      Show Abstract
      21643842
      Closed-tube PCR methods for locus-specific DNA methylation analysis.
      Ida L M Candiloro,Thomas Mikeska,Alexander Dobrovic
      Methods in molecular biology (Clifton, N.J.) 791 2011

      Show Abstract
      21913071
      Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation.
      Ida L M Candiloro,Thomas Mikeska,Alexander Dobrovic
      Epigenetics : official journal of the DNA Methylation Society 6 2011

      Show Abstract Full Text Article
      21364322
      SOCS3 promoter methylation is mutually exclusive to EGFR amplification in gliomas and promotes glioma cell invasion through STAT3 and FAK activation.
      Carina Lindemann,Oliver Hackmann,Sabit Delic,Natalie Schmidt,Guido Reifenberger,Markus J Riemenschneider
      Acta neuropathologica 122 2011

      Show Abstract
      21590492
      Constant p53 pathway inactivation in a large series of soft tissue sarcomas with complex genetics.
      Pérot G, Chibon F, Montero A, Lagarde P, de Thé H, Terrier P, Guillou L, Ranchère D, Coindre JM, Aurias A
      Am J Pathol 177 2080-90. 2010

      Show Abstract Full Text Article
      20884963
      Methylation Status of the O6-Methylguanine-Deoxyribonucleic Acid Methyltransferase Gene Promoter in World Health Organization Grade III Gliomas.
      Seung-Heon Yang,Yong Hwy Kim,Jin Wook Kim,Chul-Kee Park,Sung-Hye Park,Hee-Won Jung
      Journal of Korean Neurosurgical Society 46 2009

      Show Abstract Full Text Article
      19893731
      Capillary electrophoretic analysis of methylation status in CpG-rich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine.
      Victoria L Boyd, Gerald Zon
      Analytical biochemistry 380 13-20 2008

      Show Abstract
      18539128
      Ferrocenylnaphthalene diimide-based electrochemical detection of methylated gene.
      Shinobu Sato, Koji Hokazono, Tatsuya Irie, Takashi Ueki, Michinori Waki, Takahiko Nojima, Hiroki Kondo, Shigeori Takenaka
      Analytica chimica acta 578 82-7 2006

      Show Abstract
      17723697
      Detection of epigenetic changes in fecal DNA as a molecular screening test for colorectal cancer: a feasibility study
      Leung, Wai K, et al
      Clin Chem, 50:2179-82 (2004) 2004

      Positive Control15502094

      Brochure

      Title
      CpG MethylQuest™ DNA Isolation Kit
      Product Selection Guide: Shaping Epigenetics Discovery

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      Categories

      Life Science Research > Genomic Analysis > DNA Methylation Analysis > Methylated and Non-Methylated DNA Standards
      Life Science Research > Genomic Analysis > DNA Methylation Analysis > Methylation Specific PCR Analysis