S7821 | CpGenome Universal Methylated DNA

S7821
10 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number S7821
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      • CpGenome
      Description CpGenome Universal Methylated DNA
      Overview CpGenome™ Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.

      Materials Provided:

      Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.

      Validation:

      Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ™ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.

      CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ™ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
      Background Information Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppresser genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors.
      Several methods have been developed to determine the methylation status of cytosine. These include digestion with methylation sensitive restriction enzymes as in restriction landmark genomic scanning, oligonucleotide arrays, genomic DNA sequencing and methylation specific PCR (MSP). Some techniques are more useful for discovery while others are better used for monitoring of known methylated cytosines. Genomic DNA sequencing, although time consuming and labor intensive, offers a more universal detection method. MSP is now an established technology for the monitoring of abnormal gene methylation in selected gene sequences. Utilizing small amounts of DNA, this procedure offers sensitive and specific detection of 5-methylcytosine in promoters. It is being exploited to define tumor suppresser gene function, and to provide a new strategy for early tumor detection.
      The initial step of both genomic sequencing and MSP is to perform a bisulfite modification of the DNA sample. MSP then involves PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
      References
      Product Information
      Presentation Liquid in TE (10mM Tris-HCL, 0.1mM EDTA) with no preservatives.
      Applications
      Application Enzymatically methylated human male genomic DNA to be used as a methylation-positive control for gene methylation studies.
      Application Notes For MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Species Reactivity Human
      Modifications
      • Methylation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Recommended Storage: -15°C to -25°C, in aliquots, protected from freeze-thaws for up to two years from date of receipt. Do not freeze and thaw the same -20°C aliquot more than 3X for best results. Multiple freeze thaws can fragment DNA. Storage at -70°C can be used for longer term storage if necessary; minimize multiple freeze thaws for best results.
      Packaging Information
      Material Size 10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      English

      Certificates of Analysis

      TitleLot Number
      CpGenome Universal Methylated DNA - 21219352121935
      CpGenome Universal Methylated DNA - 20251312025131
      CpGenome Universal Methylated DNA - 20422102042210
      CpGenome Universal Methylated DNA - 20895042089504
      CpGenome Universal Methylated DNA - 22015792201579
      CpGenome™ Universal Methylated DNA - 25584372558437
      CpGenome™ Universal Methylated DNA -24756292475629
      CpGenome™ Universal Methylated DNA - 23283232328323
      CpGenome™ Universal Methylated DNA - 23956492395649
      CpGenome™ Universal Methylated DNA - 24303192430319
      CpGenome™ Universal Methylated DNA - 22763162276316

      References

      Reference overviewApplicationPub Med ID
      DNA hypermethylation and histone modifications downregulate the candidate tumor suppressor gene RRP22 on 22q12 in human gliomas.
      Natalie Schmidt,Sonja Windmann,Guido Reifenberger,Markus J Riemenschneider
      Brain pathology (Zurich, Switzerland) 22 2012

      Show Abstract
      21631628
      SOCS3 promoter methylation is mutually exclusive to EGFR amplification in gliomas and promotes glioma cell invasion through STAT3 and FAK activation.
      Carina Lindemann,Oliver Hackmann,Sabit Delic,Natalie Schmidt,Guido Reifenberger,Markus J Riemenschneider
      Acta neuropathologica 122 2011

      Show Abstract
      21590492
      Assessing combined methylation-sensitive high resolution melting and pyrosequencing for the analysis of heterogeneous DNA methylation.
      Ida L M Candiloro,Thomas Mikeska,Alexander Dobrovic
      Epigenetics : official journal of the DNA Methylation Society 6 2011

      Show Abstract Full Text Article
      21364322
      IDH1 and IDH2 mutations, immunohistochemistry and associations in a series of brain tumors.
      Mellai, Marta, et al.
      J. Neurooncol., 105: 345-57 (2011) 2011

      Show Abstract
      21643842
      Closed-tube PCR methods for locus-specific DNA methylation analysis.
      Ida L M Candiloro,Thomas Mikeska,Alexander Dobrovic
      Methods in molecular biology (Clifton, N.J.) 791 2011

      Show Abstract
      21913071
      Constant p53 pathway inactivation in a large series of soft tissue sarcomas with complex genetics.
      Pérot G, Chibon F, Montero A, Lagarde P, de Thé H, Terrier P, Guillou L, Ranchère D, Coindre JM, Aurias A
      Am J Pathol 177 2080-90. 2010

      Show Abstract Full Text Article
      20884963
      Methylation Status of the O6-Methylguanine-Deoxyribonucleic Acid Methyltransferase Gene Promoter in World Health Organization Grade III Gliomas.
      Seung-Heon Yang,Yong Hwy Kim,Jin Wook Kim,Chul-Kee Park,Sung-Hye Park,Hee-Won Jung
      Journal of Korean Neurosurgical Society 46 2009

      Show Abstract Full Text Article
      19893731
      Capillary electrophoretic analysis of methylation status in CpG-rich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine.
      Victoria L Boyd, Gerald Zon
      Analytical biochemistry 380 13-20 2008

      Show Abstract
      18539128
      Ferrocenylnaphthalene diimide-based electrochemical detection of methylated gene.
      Shinobu Sato, Koji Hokazono, Tatsuya Irie, Takashi Ueki, Michinori Waki, Takahiko Nojima, Hiroki Kondo, Shigeori Takenaka
      Analytica chimica acta 578 82-7 2006

      Show Abstract
      17723697
      Detection of epigenetic changes in fecal DNA as a molecular screening test for colorectal cancer: a feasibility study
      Leung, Wai K, et al
      Clin Chem, 50:2179-82 (2004) 2004

      Positive Control15502094

      Brochure

      Title
      CpG MethylQuest™ DNA Isolation Kit
      Product Selection Guide: Shaping Epigenetics Discovery

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      Categories

      Life Science Research > Genomic Analysis > DNA Methylation Analysis > Methylated and Non-Methylated DNA Standards
      Life Science Research > Genomic Analysis > DNA Methylation Analysis > Methylation Specific PCR Analysis