S7107 | ApopTag® TdT Enzyme

Detection of apoptotic cells with ApopTag® Red in human lymph node tissue, fixed with 10% neutral-buffered formalin and paraffin-embedded.
Detection of apoptotic cells with ApopTag® Red in human lymph node tissue, fixed with 10% neutral-bu
S7107
300 µL  1000 units/mL
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      Overview

      Replacement Information

      Key Spec Table

      Description
      Catalogue Number S7107
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      • ApopTag
      Description ApopTag® TdT Enzyme
      Overview ApopTag® Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits (Catalog #s: S7100, S7101, S7110, S7111, S7160 and S7165).
      ApopTag is a registered trademark of Serologicals Company.
      References
      Product Information
      Applications
      Application ApopTag Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection.
      Application Notes Materials Provided:
      Each vial contains 300 μl of ApopTag® Terminal Transferase (TdT) Enzyme.
      Instructions for Use

      Prepare labeling mixture as follows:
      77 μl ApopTag® Reaction Buffer (S7105 or kit component # 02)
      33 μl ApopTag® TdT Enzyme
      Mix well and keep on ice. This reagent may be prepared in advance and stored on ice for no more than 6 hours. The Reaction Buffer - TdT Enzyme mixture is ready for use at the "APPLY WORKING STRENGTH TdT ENZYME" step in The Complete ApopTag® Manual.
      Warnings and Precautions :
      ApopTag® Terminal Transferase (TdT) Enzyme contains potassium cacodylate (dimethylarsinic acid) as a buffer. This material is harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses); wash areas of contact immediately.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Recommended Storage -15°C to -25°C.
      Packaging Information
      Material Size 300 µL
      Material Package 1000 units/mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      English

      Certificates of Analysis

      TitleLot Number
      ApopTag#174; TdT Enzyme - 19887381988738
      ApopTag#174; TdT Enzyme - 20422062042206
      ApopTag#174; TdT Enzyme - 20890062089006
      ApopTag#174; TdT Enzyme - 21959452195945
      ApopTag#174; TdT Enzyme - 22688192268819
      ApopTag® TdT Enzyme - 24304032430403
      ApopTag® TdT Enzyme - 24595442459544
      ApopTag® TdT Enzyme - 23090822309082
      ApopTag® TdT Enzyme - 23659752365975
      ApopTag® TdT Enzyme - 25064672506467
      ApopTag® TdT Enzyme -25833932583393
      ApopTag® TdT Enzyme -25938652593865

      References

      Reference overviewPub Med ID
      Notch signaling controls the balance of ciliated and secretory cell fates in developing airways.
      Po-Nien Tsao,Michelle Vasconcelos,Konstantin I Izvolsky,Jun Qian,Jining Lu,Wellington V Cardoso
      Development (Cambridge, England) 136 2009

      Show Abstract Full Text Article
      19502490