PMEF-CF | EmbryoMax® Primary Mouse Embryo Fibroblasts, Strain CF1, Mytomycin C treated, passage 3

PMEF-CF
5 vials  5-6x106 ea
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      Overview

      Key Spec Table

      Key Applications
      Cult
      Description
      Catalogue Number PMEF-CF
      Synonyms Non drug resistant cells from CF-1 mice- Mitomycin C treated
      Trade Name
      • Specialty Media
      • Embryomax
      Description EmbryoMax® Primary Mouse Embryo Fibroblasts, Strain CF1, Mytomycin C treated, passage 3
      Overview PMEF feeders are treated with Mitomycin-C and can be directly plated onto gelatin coated (See ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 106 cells will produce the appropriate density feeder layer on the following tissue culture vessels:

      6 x 60mm dishes

      2 x T75's

      2 x 6 well plates



      See ES Stem Cell Manual for complete culture and media for PMEF cells
      References
      Product Information
      Applications
      Application The PMEF feeders are treated with Mitomycin-C. These fibroblasts can be directly plated onto gelatin coated (See ES-006-B) culture vessels.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Cell Line Type
      • Primary Mouse Embryo Fibroblasts
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
      Packaging Information
      Material Size 5 vials
      Material Package 5-6x106 ea
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2040624-1 2040624-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2048176-1 2048176-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2010171-1 2010171-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2043018-1 2043018-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2077214-1 2077214-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2116040-1 2116040-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 01030-4 01030-4
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-4 10226-4
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-5 10226-5
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-6 10226-6
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-7 10226-7
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-8 10226-8
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10226-9 10226-9
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10326-2 10326-2
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10326-3 10326-3
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10326-6 10326-6
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10326-7 10326-7
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10326-8 10326-8
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10326-9 10326-9
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10405-4 10405-4
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 10412-8 10412-8
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 1948394 1948394
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 1949226 1949226
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 1949227 1949227
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 1949229 1949229
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 1949230 1949230
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 1990099 1990099
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2000569 2000569
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2011198 2011198
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2021871 2021871
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2023449 2023449
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2026147 2026147
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2028726 2028726
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2035335 2035335
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2043019-1 2043019-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2048170 2048170
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2059982-1 2059982-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2060006 2060006
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2071023 2071023
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2071049-1 2071049-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2116040-2 2116040-2
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2140354-1 2140354-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2142750-1 2142750-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - 2148484-1 2148484-1
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - DAM1947055 DAM1947055
      EmbryoMax#174; PMEF Cells Strain CF-1, Mito-C Treated - DAM1949225 DAM1949225
      EmbryoMax® PMEF Cells 2119159-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2109128-1 2109128-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2314966-1 2314966-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2322599-1 2322599-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2326963-1 2326963-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2341188-1 2341188-1
      EmbryoMax® PMEF Cells -2077214-2 2077214-2
      EmbryoMax® PMEF Cells -2458242-2 2458242-2
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2048175-1 2048175-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2165072-1 2165072-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2212269-1 2212269-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2234925-1 2234925-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2234926-1 2234926-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated - 2281112-1 2281112-1
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated -2148484-2 2148484-2
      EmbryoMax® PMEF Cells Strain CF-1, Mito-C Treated -2365456-2 2365456-2

      References

      Reference overviewPub Med ID
      Transcriptional signature and memory retention of human-induced pluripotent stem cells.
      Marchetto, Maria C N, et al.
      PLoS ONE, 4: e7076 (2009) 2009

      Show Abstract
      19763270
      Establishment of rat embryonic stem cells and making of chimera rats.
      Ueda S. et al.
      PLoS One 3(7) e2800 2008

      Show Abstract
      18665239

      Brochure

      Title
      Murine Embryonic Stem Cell Culture Procedures & Protocols

      Technical Info

      Title
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs