MABN69 | Anti-Caspr Antibody, clone K65/35

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded rat cerebellum (Fig. 1) and rat hippocampus (Fig. 2) tissues were prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of Cat. No. MABN69, Anti-Caspr, clone K65/35. Reactivity was detected using the IHC-Select Detection Kit (Cat. No. DAB050). Staining appears to be restricted to the white matter/myelinated axons (granular layer junction) in Fig. 1and cellular processes in Fig. 2.
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded rat cerebellum
MABN69
100 µg  
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      Overview

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      RWB,IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MABN69
      Synonyms Neurexin-4 contactin associated protein 1 neurexin 4 neurexin-4 contactin-associated protein 1 Neurexin IV
      Description Anti-Caspr Antibody, clone K65/35
      Background Information Contactin-associated protein 1(Caspr) is a single-pass type I membrane protein belonging to the neurexin family and is comprised of one F5/8 type C domain, one fibrinogen C-terminal domain, two EGF-like domains, and four laminin G-like domains. Caspr is well documented for its interaction with contactin, an interaction that is crucial to the transport of Caspr to the plasma membrane, the mylination of glial cells, and the generation of axoglial junctions. Studies also suggest that Caspr may play a regulatory role in the cell surface expression of contactin and its targeting to different axonal domains. Expression of Caspr is largely observed in the brain, mainly in CNS myelinated nerve fibers within paranodal axoglial junctions.
      References
      Product Information
      Format Purified
      Control
      • Rat brain membrane tissue lysate
      Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application Anti-Caspr Antibody, clone K65/35 is an antibody against Caspr for use in IH & WB.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application Notes Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Caspr in rat cerebellum and rat hippocampus tissues.
      Biological Information
      Immunogen Recombinant protein corresponding to rat Caspr.
      Clone K65/35
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Isotype IgG1κ
      Species Reactivity Rat
      Species Reactivity Note Demonstrated to react with rat. Predicted to react with mouse based on 100% sequence homology.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Forms a complex with contactin; may mediate communication between glial cells and neurons during development [RGD].
      Gene Symbol
      • CASPR
      • CNTNAP
      • Caspr1
      • caspr1
      • P190
      • p190
      • NRXN4
      Purification Method Protein G
      UniProt Number
      UniProt Summary FUNCTION: Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.

      SUBUNIT STRUCTURE: Interacts with contactin in cis form.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential).

      TISSUE SPECIFICTY: Predominantly expressed in brain. In myelinated nerve fibers of the CNS predominantly found in paranodal axoglial junctions. In unmyelinated nerve fibers of the CNS diffusely distributed along the entire surface. Weak expression is detected in ovary, pancreas, colon, lung, heart, intestine and testis.

      DEVELOPMENTAL STAGE: Detected on postnatal day 7 in cerebellum. Follows a caudorostral progession according to the myelination process. Appears to redistribute from the internode to the paranodal region during myelin compaction and maturation. Expression reaches maximal levels between days 14 and 18 and remains at the same levels until adulthood.

      SEQUENCE SIMILARITIES: Belongs to the neurexin family.

      Contains 2 EGF-like domains.

      Contains 1 F5/8 type C domain.

      Contains 1 fibrinogen C-terminal domain.

      Contains 4 laminin G-like domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blot in rat brain membrane tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Caspr on 10 µg of rat brain membrane tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications