MABN41 | Anti-Sodium channel Nav1.7 Antibody, clone N68/6

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded inflammatory rat cerebellum tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:400 dilution of Cat. No. MABN41, Anti-Sodium channel Nav1.7, clone N68/6. Reactivity was detected using the IHC-Select Detection Kit (Cat. No. DAB050). Staining appears localized to the basket cells above Purkinje cells at the ganular layer junction.
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded inflammatory r
MABN41
100 μg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MABN41
      Synonyms Neuroendocrine sodium channel sodium channel, voltage-gated, type IX, alpha subunit Sodium channel protein type IX subunit alpha Voltage-gated sodium channel subunit alpha Nav1.7 Peripheral sodium channel 1 sodium channel, voltage-gated, type IX, alpha polypeptide Nav1.7 sodium channel protein type 9 subunit alpha voltage-gated sodium channel alpha subunit Nav1.7
      Description Anti-Sodium channel Nav1.7 Antibody, clone N68/6
      Background Information Voltage-gated sodium channel subunit alpha Nav1.7 (Nav1.7) is a multi-pass membrane protein containing a single IQ domain that belongs to the sodium channel family. NAV1.7 localizes to the terminal ends of sensory neurons and is believed to have a role in inflammatory pain development and mechanisms. It serves to regulate excitable membrane voltage-dependent Na+ ion permeability. There is a strong probability that Nav1.7 is involved in the transmission of nociceptive information, a suggested involvement supported by its role in a variety of chronic pain neuropathies induced by damage to the peripheral nerves. Nav1.7 is predominantly observed in dorsal root ganglion, and sensory and sympathetic neurons. Lower levels of the protein have been observed in MTC cell line, C-cell carcinoma, and smooth muscle cells. Defects in Nav1.7 expression are causal to several diseases such as autosomal recessive congenital indifference pain, primary erythermalgia, and paroxysmal extreme pain disorder or PEPD.
      References
      Product Information
      Format Purified
      Control
      • Rat cerebellum tissue
      Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application This Anti-Sodium channel Nav1.7 Antibody, clone N68/6 is validated for use in IH, WB for the detection of Sodium channel Nav1.7.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application Notes Western Blot Analysis: A previous lot was used by an independent laboratory in HEK293 cell lysate. (James Trimmer, UC Davis/NIH NeuroMab Facility, Department of Neurobiology, Physiology and Behavior, UC Davis, Davis CA 95616-8519. neuromab@ucdavis.edu.)
      Biological Information
      Immunogen species Recombinant protein corresponding to human Nav1.7.
      Clone N68/6
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Isotype IgG1κ
      Species Reactivity Human Rat
      Species Reactivity Note Demonstrated to react with rat and human.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq].
      Gene Symbol
      • SCN9A
      • NENA
      • PN1
      • hNE-Na
      • FEB3B
      • NE-NA
      • ETHA
      Purification Method Protein G
      UniProt Number
      UniProt Summary FUNCTION: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (By similarity).
      SIZE: 1988 amino acids; 226342 Da

      SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4 and NEDD4L (By similarity).

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=In neurite terminals (By similarity).

      TISSUE SPECIFICITY: Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system while isoform 2 is expressed preferentially in the dorsal root ganglion.

      DOMAIN: The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

      PTM: Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4 (By similarity).

      DISEASE: Defects in SCN9A are the cause of primary erythermalgia [MIM:133020]. It is an autosomal dominant disease characterized by recurrent episodes of severe pain associated with redness and warmth in the feet or hands. & Defects in SCN9A are the cause of autosomal recessive congenital indifference to pain [MIM:243000]; also known as channelopathy-associated insensitivity to pain. Affected individuals have a congenital inability to perceive any form of pain, in any part of the body. All other sensory modalities are preserved and the peripheral and central nervous systems are apparently intact. Patients perceive the sensations of touch, warm and cold temperature, proprioception, tickle and pressure, but not painful stimuli. There is no evidence of a motor or sensory neuropathy, either axonal or demyelinating. & Defects in SCN9A are a cause of paroxysmal extreme pain disorder (PEPD) [MIM:167400]; previously known as familial rectal pain (FRP). PEPD is an autosomal dominant paroxysmal disorder of pain and autonomic dysfunction. The distinctive features are paroxysmal episodes of burning pain in the rectal, ocular, and mandibular areas accompanied by autonomic manifestations such as skin flushing.

      SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Immunohistochemistry in rat cerebellum tissue.

      Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Nav1.7 in rat cerebellum tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size 100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Sodium channel Nav1.7, clone N68/6 - 2343902 2343902
      Anti-Sodium channel Nav1.7, clone N68/6 - 2395804 2395804
      Anti-Sodium channel Nav1.7, clone N68/6 - 2166826 2166826
      Anti-Sodium channel Nav1.7, clone N68/6 - 1948289 1948289
      Anti-Sodium channel Nav1.7, clone N68/6 - 2092736 2092736
      Anti-Sodium channel Nav1.7, clone N68/6 - 2197305 2197305
      Anti-Sodium channel Nav1.7, clone N68/6 - 2295498 2295498
      Anti-Sodium channel Nav1.7, clone N68/6 - NG1940984 NG1940984
      Anti-Sodium channel Nav1.7, clone N68/6 - NRG1794369 NRG1794369

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.