MAB4365 | GCTM-5 Antibody, clone GCTM-5

CFPAC1 cells fixed and labeled with 5 μg/mL of GCTM-5 antibody (green); cells are also labeled with DAPI (blue) to visualize all nuclei. A subset of the cells are labeled with GCTM-5, a normal pattern of staining for this antibody. The GCTM-5 antibody labeling was visualized with an Alexa 488-conjugated goat anti-mouse secondary antibody.
CFPAC1 cells fixed and labeled with 5 μg/mL of GCTM-5 antibody (green); cells are also labeled with
MAB4365
100 µg  
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      Overview

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      HWB,ICC,IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB4365
      Trade Name
      • Chemicon
      Description GCTM-5 Antibody, clone GCTM-5
      Background Information The GCTM-5 monoclonal antibody reacts with a minority sub-population of cells in spontaneously differentiating cultures of pluripotent human embryonic stem cells and embryonal carcinoma. The epitope recognized by GCTM-5 is found on a 50-kDa protein present on the surface of these cells. Previous studies suggest that GCTM-5 may also prove to be a useful tool for defining cell lineage relationships between putative progenitor populations in embryonic liver and biliary epithelium during tissue repair (Pera M, et al, 2005).
      References
      Product Information
      Format Purified
      Specificity This antibody reacts with GCTM-5 antigen that is expressed upon the cell surface of human embryonal carcinoma, differentiating cultures of human embryonic stem cells, and bilary epithelial cells.
      Presentation Purified mouse monoclonal IgG1 with 0.05% sodium azide.
      Applications
      Application GCTM-5 Antibody, clone GCTM-5 is an antibody against GCTM-5 for use in IC, IH & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Biological Information
      Immunogen Membrane prepared from testicular seminoma
      Clone GCTM-5
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity This antibody reacts with GCTM-5 antigen that is expressed upon the cell surface of human embryonal carcinoma, differentiating cultures of human embryonic stem cells, and bilary epithelial cells.
      Isotype IgG1
      Species Reactivity Human
      Species Reactivity Note Reacts with human. Reactivity to other species has not yet been determined.
      Antibody Type Monoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Languages
      English

      Certificates of Analysis

      TitleLot Number
      GCTM-5 Antibody, clone GCTM-5 - 2109546 2109546
      GCTM-5 Antibody, clone GCTM-5 - 2283549 2283549
      Monoclonal Antibody - LV1478901 LV1478901

      References

      Reference overviewPub Med ID
      Transcriptional analysis of early lineage commitment in human embryonic stem cells.
      Laslett, Andrew L, et al.
      BMC Dev. Biol., 7: 12 (2007) 2007

      Show Abstract
      17335568
      Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
      Adewumi O, et al.
      Nat. Biotechnol., 25: 803-16 (2007) 2007

      17572666
      Liver stem cells--prospects for clinical use.
      Tosh, David and Strain, Alastair
      J. Hepatol., 42 Suppl: S75-84 (2005) 2005

      15777575
      The hESC line Envy expresses high levels of GFP in all differentiated progeny.
      Costa, Magdaline, et al.
      Nat. Methods, 2: 259-60 (2005) 2005

      15782217
      A novel cell-surface marker found on human embryonic hepatoblasts and a subpopulation of hepatic biliary epithelial cells.
      Stamp, Lincon, et al.
      Stem Cells, 23: 103-12 (2005) 2005

      15625127

      Brochure

      Title
      Human Stem Cell Systems

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies