MAB4334 | Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

MAB4334
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, PmIHC, IP, IF, FC, FUNC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB4334
      Brand Family Chemicon®
      Synonyms P-glycoprotein; CD243; p170; Pgp
      Trade Name
      • Chemicon
      Description Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2
      Background Information UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE's UIC2 Shift assay - see product ECM905).
      References
      Product Information
      Format Purified
      Specificity P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
      Presentation The immunoglobulin was purified by protein A Sepharose™ chromatography and is presented as a liquid in in 0.1M PBS, containing 0.1% sodium azide as a preservative.
      Applications
      Application Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2 detects level of MDR1 & has been published & validated for use in FC, IF, IP, IH, IH(P).
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Immunofluorescence
      • Flow Cytometry
      • Affects Function
      • Immunohistochemistry (Paraffin)
      Application Notes Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.

      Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.

      Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.

      Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.

      IHC on frozen and paraffin sections: 1 - 10 μg/mL.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen species Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.
      Epitope conformational extracellular epitope
      Clone UIC2
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)
      Isotype IgG2aκ
      Species Reactivity Human Primate
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
      Gene Symbol
      • ABCB1
      • EC 3.6.3.44
      • ABC20
      • CLCS
      • MDR1
      • P-gp
      • GP170
      • CD243
      • MGC163296
      • PGY1
      Non-Reactive Species
      • Mouse
      • Rat
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P08183 # Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
      SIZE: 1280 amino acids; 141463 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in liver, kidney, small intestine and brain.
      SIMILARITY: SwissProt: P08183 ## Belongs to the ABC transporter family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain at 2-8°C for up 12 months from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Mouse Anti-MDR1 (P-glycoprotein) UIC2 Monoclonal Antibody - 2025983 2025983
      Mouse Anti-MDR1 (P-glycoprotein) UIC2 Monoclonal Antibody - 2191961 2191961
      Mouse Anti-MDR1 (P-glycoprotein) UIC2 Monoclonal Antibody - 2341086 2341086

      References

      Reference overviewPub Med ID
      Human Liver Stem Cell-Derived Microvesicles Inhibit Hepatoma Growth in SCID Mice by Delivering Antitumor MicroRNAs.
      Valentina Fonsato,Federica Collino,Maria Beatriz Herrera,Claudia Cavallari,Maria Chiara Deregibus,Barbara Cisterna,Stefania Bruno,Renato Romagnoli,Mauro Salizzoni,Ciro Tetta,Giovanni Camussi
      Stem cells (Dayton, Ohio) 30 2012

      Show Abstract
      22736596
      Activity of the Bcl-2 family inhibitor ABT-263 in a panel of small cell lung cancer xenograft models.
      Alex R Shoemaker,Michael J Mitten,Jessica Adickes,Scott Ackler,Marion Refici,Debra Ferguson,Anatol Oleksijew,Jacqueline M O'Connor,Baole Wang,David J Frost,Joy Bauch,Kennan Marsh,Steven K Tahir,Xiufen Yang,Christin Tse,Stephen W Fesik,Saul H Rosenberg,Steven W Elmore
      Clinical cancer research : an official journal of the American Association for Cancer Research 14 2008

      Show Abstract
      18519752
      Levels of multidrug resistance (MDR1) P-glycoprotein expression by human breast cancer correlate with in vitro resistance to taxol and doxorubicin.
      Mechetner, E, et al.
      Clin. Cancer Res., 4: 389-98 (1998) 1998

      Show Abstract
      9516927

      Data Sheet

      Title
      Anti-MDR1, conformational extracellular epitope, clone UIC2 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies