MAB424 | Anti-PCNA Antibody, clone PC10

PCNA (Catalog Number MAB424, Clone PC10) staining of human tonsil, tissue pretreated with citrate buffer, pH 6.0, antibody at 1:1500, IHC Select® detection with HRP-DAB. Marginal tissues of the tonsil are highly immunoreactive.
PCNA (Catalog Number MAB424, Clone PC10) staining of human tonsil, tissue pretreated with citrate bu
MAB424
500 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Inv, VrtELISA, WB, IP, IH(P), FCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB424
      Brand Family Chemicon®
      Synonyms Proliferating Cell Nuclear Antigen; DNA Polymerase delta Processivity Factor
      Trade Name
      • Chemicon
      Description Anti-PCNA Antibody, clone PC10
      Background Information Proliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells.
      References
      Product Information
      Format Purified
      Specificity Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.
      Control
      • Rat kidney, Human tonsil, lymph node tissue
      Presentation Protein A Purified mouse immunoglobulin in PBS with 0.1% sodium azide as a preservative.
      Applications
      Application Use Anti-PCNA Antibody, clone PC10 (mouse monoclonal antibody) validated in ELISA, FC,IHC(P), IP, WB to detect PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application Notes Western blot: 1:250-1:1000. Nuclear extracts are preferred over whole cell protein extracts.

      Immunohistochemistry: 1:20-1:200. Recommended for paraffin embedded tissue sections only. May be used on material fixed in a wide range of fixatives including formalin (buffered and unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air-drying overnight on poly-L-lysine coated slides is recommended. 60 minute incubation at 25°C with standard ABC technique is recommended.

      Immunoprecipitation

      Indirect Flow Cytometry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen species Rat PCNA made in the protein A vector pR1T2T.
      Clone PC10
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.
      Isotype IgG2a
      Species Reactivity Invertebrates Vertebrates
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
      Gene Symbol
      • PCNA
      • MGC8367
      • cyclin
      • Cyclin
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P12004 # This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
      SIZE: 261 amino acids; 28769 Da
      SUBUNIT: Homotrimer. Interacts with KCTD10 (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Upon methyl methanesulfonate-induced DNA damage, mono- ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
      DISEASE:SwissProt: P12004 # Antibodies are present in sera from patients with systemic lupus erythematosus.
      SIMILARITY: SwissProt: P12004 ## Belongs to the PCNA family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size 500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI- PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY - 2006035 2006035
      MOUSE ANTI- PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY - 2051862 2051862
      MOUSE ANTI- PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY - 2147073 2147073
      MOUSE ANTI- PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) MONOCLONAL ANTIBODY - 2280426 2280426

      References | 18 Available | See All References

      Reference overviewPub Med ID
      Inhibition of HIV-1 Tat-mediated transcription by a coumarin derivative, BPRHIV001, through the Akt pathway.
      Lin PH, Ke YY, Su CT, Shiao HY, Hsieh HP, Chao YK, Lee CN, Kao CL, Chao YS, Chang SY
      Journal of virology 85 9114-26. Epub 2011 Jun 22. 2011

      Show Abstract
      21697490
      Oligodeoxynucleotide IMT504 induces a marked recovery in a streptozotocin-induced model of diabetes in rats: correlation with an early increase in the expression of nestin and neurogenin 3 progenitor cell markers.
      MS Bianchi, A Hernando-Insua, NA Chasseing, JM Rodriguez, F Elias, N Lago, J Zorzopulos, C Libertun, AD Montaner, VA Lux-Lantos
      Diabetologia 2010

      Show Abstract
      20221823
      RanBPM Has Proapoptotic Activities That Regulate Cell Death Pathways in Response to DNA Damage.
      Atabakhsh, Elnaz, et al.
      Mol. Cancer Res., (2009) 2009

      Show Abstract
      19996306
      Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain.
      Chiaki Ohtaka-Maruyama, Akiko Miwa, Hitoshi Kawano, Masataka Kasai, Haruo Okado
      The Journal of comparative neurology 502 1098-108 2007

      Show Abstract
      17447250
      Mitogenic effects of the up-regulation of minichromosome maintenance proteins in anaplastic thyroid carcinoma.
      Teresa Guida, Giuliana Salvatore, Pinuccia Faviana, Riccardo Giannini, Ginesa Garcia-Rostan, Livia Provitera, Fulvio Basolo, Alfredo Fusco, Francesca Carlomagno, Massimo Santoro
      The Journal of clinical endocrinology and metabolism 90 4703-9 2005

      Show Abstract
      15899946
      Histochemical and immunohistochemical localisation of elastic system fibres in focal reactive overgrowths of oral mucosa.
      A J Mighell, P A Robinson, W J Hume, A J Mighell, P A Robinson, W J Hume, A J Mighell, P A Robinson, W J Hume
      Journal of oral pathology medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 26 153-8 1997

      Show Abstract
      9176788
      Immunolocalisation of tenascin-C in focal reactive overgrowths of oral mucosa.
      A J Mighell, P A Robinson, W J Hume
      Journal of oral pathology medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 25 163-9 1996

      Show Abstract
      8809684
      PCNA and Ki-67 immunoreactivity in multinucleated cells of giant cell fibroma and peripheral giant cell granuloma.
      A J Mighell, P A Robinson, W J Hume
      Journal of oral pathology medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 25 193-9 1996

      Show Abstract
      8835814
      PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines.
      Coltrera, M D and Gown, A M
      J. Histochem. Cytochem., 39: 23-30 (1991) 1991

      Show Abstract
      1670579
      Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
      Waseem, N H and Lane, D P
      J. Cell. Sci., 96 ( Pt 1): 121-9 (1990) 1990

      Show Abstract
      1695635

      Data Sheet

      Title
      Anti-PCNA, clone PC10 - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies