MAB374 | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

Immunocytochemistry Analysis:
Representative images from a previous lot.
Confolcal fluorescent analysis of HeLa and NIH/3T3 cells using MAB374 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Cytosol staining
Immunocytochemistry Analysis:
Representative images from a previous lot.
Confolcal fluores
MAB374
200 μg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Po, Ca, H, M, R, Rb, F, FeWB, ELISA, IP, ICC, IF, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB374
      Brand Family Chemicon®
      Synonyms EC 1.2.1.12 G3PD MGC88685 OK/SW-cl.12 GAPD G3PDH GAPDH
      Trade Name
      • Chemicon
      Description Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
      Background Information Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      Format Purified
      Specificity Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
      Control
      • It is expressed in all cells.
      Presentation Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.
      Applications
      Application Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 detects level of Glyceraldehyde-3-Phosphate Dehydrogenase & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      Application Notes ELISA:
      1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

      Immunocytochemistry:
      1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

      Western blot:
      1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

      Immunoaffinity purification of GAPDH.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen species Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
      Clone 6C5
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
      Isotype IgG1
      Species Reactivity Pig Canine Human Mouse Rat Rabbit Fish Feline
      Species Reactivity Note Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle.
      It has been reported that this antibody does not react with GAPDH from E. coli.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
      Gene Symbol
      • true
      Non-Reactive Species
      • E. coli
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
      SIZE: 335 amino acids; 36053 Da
      SUBUNIT: Homotetramer. Binds PRKCI.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.
      SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size 200 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

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      Certificates of Analysis

      TitleLot Number
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2145925 2145925
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2388833 2388833
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2430317 2430317
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 1990892 1990892
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2271144 2271144
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2322571 2322571
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 2506322 2506322
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1604282 JC1604282
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1641540 JC1641540
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1682928 JC1682928
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - LV1555185 LV1555185
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - LV1581114 LV1581114
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1721601 NG1721601
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1740950 NG1740950
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1741566 NG1741566
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1780785 NG1780785
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1839548 NG1839548
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1857556 NG1857556
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1869834 NG1869834
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2470405 2470405
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -2521487 2521487
      MOUSE ANTI-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MONOCLONAL ANTIBODY -

      References | 72 Available | See All References

      Reference overviewPub Med ID
      Partial restoration of cardiac function with ΔPDZ nNOS in aged mdx model of Duchenne cardiomyopathy.
      Lai, Yi, et al.
      Hum. Mol. Genet., (2014) 2014

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      24463882
      Effect of pirfenidone on proliferation, TGF-β-induced myofibroblast differentiation and fibrogenic activity of primary human lung fibroblasts.
      Conte, Enrico, et al.
      Eur J Pharm Sci, 58C: 13-19 (2014) 2014

      Show Abstract
      24613900
      Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics.
      Mammoto, Akiko, et al.
      Nat Commun, 4: 1759 (2013) 2013

      Show Abstract
      23612300
      Identification of hamster inducible nitric oxide synthase (iNOS) promoter sequences that influence basal and inducible iNOS expression.
      Omar A Saldarriaga,Bruno L Travi,Goutam Ghosh Choudhury,Peter C Melby
      Journal of leukocyte biology 92 2012

      Show Abstract
      22517919
      Mouse Tissues that Undergo Neoplastic Progression after K-Ras Activation Are Distinguished by Nuclear Translocation of phospho-Erk1/2 and Robust Tumor Suppressor Responses.
      Neha Parikh,Ryan L Shuck,Thuy-Ai Nguyen,Alan Herron,Lawrence A Donehower
      Molecular cancer research : MCR 10 2012

      Show Abstract
      22532587
      Disruption of RAB40AL function leads to Martin--Probst syndrome, a rare X-linked multisystem neurodevelopmental human disorder.
      Jirair Krikor Bedoyan,Valerie M Schaibley,Weiping Peng,Yongsheng Bai,Kajari Mondal,Amol C Shetty,Mark Durham,Joseph A Micucci,Arti Dhiraaj,Jennifer M Skidmore,Julie B Kaplan,Cindy Skinner,Charles E Schwartz,Anthony Antonellis,Michael E Zwick,James D Cavalcoli,Jun Z Li,Donna M Martin
      Journal of medical genetics 49 2012

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      22581972
      Elevated cyclin g2 expression intersects with DNA damage checkpoint signaling and is required for a potent g2/m checkpoint arrest response to Doxorubicin.
      Maike Zimmermann,Aruni S Arachchige-Don,Michaela S Donaldson,Robert F Dallapiazza,Colleen E Cowan,Mary C Horne
      The Journal of biological chemistry 287 2012

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      22589537
      Effects of HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin (17-AAG) on NEU/HER2 overexpressing mammary tumours in MMTV-NEU-NT mice monitored by Magnetic Resonance Spectroscopy.
      Loreta M Rodrigues,Yuen-Li Chung,Nada M S Al Saffar,Swee Y Sharp,Laura E Jackson,Udai Banerji,Marion Stubbs,Martin O Leach,John R Griffiths,Paul Workman
      BMC research notes 5 2012

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      22621282
      Impairments to the GH-IGF-I Axis in hSOD1G93A Mice Give Insight into Possible Mechanisms of GH Dysregulation in Patients with Amyotrophic Lateral Sclerosis.
      F J Steyn,S T Ngo,J D Lee,J W Leong,A J Buckley,J D Veldhuis,P A McCombe,C Chen,M C Bellingham
      Endocrinology 153 2012

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      22621959
      Impaired contractility and detrusor hypertrophy in cavin-1-deficient mice.
      Mardjaneh Sadegh Karbalaei,Catarina Rippe,Sebastian Albinsson,Mari Ekman,Alva Mansten,Bengt Uvelius,Karl Swärd,Karl Sw
      European journal of pharmacology 689 2012

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      22643325

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