MAB374 | Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

Immunocytochemistry Analysis:
Representative images from a previous lot.
Confolcal fluorescent analysis of HeLa and NIH/3T3 cells using MAB374 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Cytosol staining
Immunocytochemistry Analysis:
Representative images from a previous lot.
Confolcal fluores
MAB374
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      Ca, Po, Fe, F, Rb, R, M, HELISA, WB, IHC, IF, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB374
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
      Alternate Names
      • glyceraldehyde 3-phosphate dehydrogenase
      • EC 1.2.1.12
      • G3PD
      • MGC88685
      • OK/SW-cl.12
      • GAPD
      • G3PDH
      • GAPDH
      • glyceraldehyde-3-phosphate dehydrogenase
      • aging-associated gene 9 protein
      Background Information Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
      References
      Product Information
      Format Purified
      Control
      • It is expressed in all cells.
      Presentation Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.
      Applications
      Application Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 is a well published and extensively characterized monoclonal antibody. This purified mAb detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      • Immunocytochemistry
      • Immunoprecipitation
      Application Notes ELISA:
      1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

      Immunocytochemistry:
      1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

      Western blot:
      1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

      Immunoaffinity purification of GAPDH.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
      Clone 6C5
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
      Isotype IgG1
      Species Reactivity Canine Pig Feline Fish Rabbit Rat Mouse Human
      Species Reactivity Note Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle.
      It has been reported that this antibody does not react with GAPDH from E. coli.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
      Gene Symbol
      • GAPDH
      • G3PD
      • GAPD
      • G3PDH
      Non-Reactive Species
      • E. coli
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
      SIZE: 335 amino acids; 36053 Da
      SUBUNIT: Homotetramer. Binds PRKCI.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.
      SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size 200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

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      Certificates of Analysis

      TitleLot Number
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 21459252145925
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 23888332388833
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 24303172430317
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 19908921990892
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 22711442271144
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 23225712322571
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - 25063222506322
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1604282JC1604282
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1641540JC1641540
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - JC1682928JC1682928
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - LV1555185LV1555185
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - LV1581114LV1581114
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1721601NG1721601
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1740950NG1740950
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1741566NG1741566
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1780785NG1780785
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1839548NG1839548
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1857556NG1857556
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 - NG1869834NG1869834
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -24704052470405
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -25214872521487
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -25715832571583
      Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5 -26063522606352
      MOUSE ANTI-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE MONOCLONAL ANTIBODY -

      References | 72 Available | See All References

      Reference overviewPub Med ID
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      Lai, Yi, et al.
      Hum. Mol. Genet., (2014) 2014

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      Effect of pirfenidone on proliferation, TGF-β-induced myofibroblast differentiation and fibrogenic activity of primary human lung fibroblasts.
      Conte, Enrico, et al.
      Eur J Pharm Sci, 58C: 13-19 (2014) 2014

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      Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics.
      Mammoto, Akiko, et al.
      Nat Commun, 4: 1759 (2013) 2013

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      Differential expression of molecular markers of synaptic plasticity in the hippocampus, prefrontal cortex, and amygdala in response to spatial learning, predator exposure, and stress-induced amnesia.
      Phillip R Zoladz,Collin R Park,Joshua D Halonen,Samina Salim,Karem H Alzoubi,Marisa Srivareerat,Monika Fleshner,Karim A Alkadhi,David M Diamond
      Hippocampus 22 2012

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      21538655
      Elevated cyclin g2 expression intersects with DNA damage checkpoint signaling and is required for a potent g2/m checkpoint arrest response to Doxorubicin.
      Maike Zimmermann,Aruni S Arachchige-Don,Michaela S Donaldson,Robert F Dallapiazza,Colleen E Cowan,Mary C Horne
      The Journal of biological chemistry 287 2012

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      Age-related decrease in cerebrovascular-derived neuroprotective proteins: Effect of acetaminophen.
      Debjani Tripathy,Alma Sanchez,Xiangling Yin,Joseph Martinez,Paula Grammas
      Microvascular research 84 2012

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      22944728
      Gene network and pathway analysis of mice with conditional ablation of dicer in post-mitotic neurons.
      Véronique Dorval,Pascal Y Smith,Charlotte Delay,Ezequiel Calvo,Emmanuel Planel,Nadège Zommer,Luc Buée,Sébastien S Hébert,V Dorval,Nad Zommer,Luc Bu,S H
      PloS one 7 2012

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      22952873
      Effects of HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin (17-AAG) on NEU/HER2 overexpressing mammary tumours in MMTV-NEU-NT mice monitored by Magnetic Resonance Spectroscopy.
      Loreta M Rodrigues,Yuen-Li Chung,Nada M S Al Saffar,Swee Y Sharp,Laura E Jackson,Udai Banerji,Marion Stubbs,Martin O Leach,John R Griffiths,Paul Workman
      BMC research notes 5 2012

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      22621282
      The G60S Cx43 mutant enhances keratinocyte proliferation and differentiation.
      Jared M Churko,John J Kelly,Andrew Macdonald,Jack Lee,Jacinda Sampson,Donglin Bai,Dale W Laird
      Experimental dermatology 21 2012

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      22775996
      Pax6 is a key component of regulated glucagon secretion.
      Yvan Gosmain,Claire Cheyssac,Mounia Heddad Masson,Audrey Guérardel,Caroline Poisson,Jacques Philippe
      Endocrinology 153 2012

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