MAB353 | Anti-Nestin Antibody, clone rat-401

Mouse anti-Nestin (Catalogue No. MAB353). Immunolocalization of Nestin in mouse brain sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin. The blue is Nissl stain (rough ER), the red is nestin (MAB353), and the green is either lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes). Photo courtesy of Robert Wine, NIEHS.
Mouse anti-Nestin (Catalogue No. MAB353). Immunolocalization of Nestin in mouse brain sections (hipp
MAB353
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      R, MWB, ICC, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB353
      Brand Family Chemicon®
      Synonyms rat401
      Trade Name
      • Chemicon
      Description Anti-Nestin Antibody, clone rat-401
      Background Information Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
      References
      Product Information
      Format Purified
      Control
      • Mouse embryonic brain tissue
      Presentation Purified mouse monoclonal IgG1 in buffer containing 0.09% sodium azide.
      Applications
      Application Detect Nestin using this Anti-Nestin Antibody, clone rat-401 validated for use in IC, IH, IH(P) & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application Notes Western Blot:
      200-220 kDa in reducing gels of newborn rat or mouse cell extracts. Note that nestin was originally reported (erroneously) to migrate at approximately 440 kDa by SDS-PAGE (Kachinsky, 1995).
      For Westerns, prepare samples by boiling in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -85°C will show breakdown of nestin sometimes into multiple fragments.

      Immunohistochemistry:
      1:20-1:200 dilution of a previous lot was used. Perfuse tissue with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM. Suitable for frozen or paraffin-embedded sections.

      Immunocytochemistry:
      1:20-1:200 dilution of a previous lot was used. Fix cells in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      Immunogen Nestin purified from embryonic rat spinal cord.
      Clone rat-401
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Rodent Nestin protein confirmed with rat and mouse. Rat-401 is not reactive with human nestin protein.
      Isotype IgG1
      Species Reactivity Rat Mouse
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
      Gene Symbol
      • Nbla00170
      • NES
      • ORFNames=Nbla00170
      • FLJ21841
      Non-Reactive Species
      • Human
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).
      SIZE: 1621 amino acids; 177439 Da
      SUBUNIT: Interacts with FHOD3 (By similarity).
      TISSUE SPECIFICITY: CNS stem cells.
      DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
      SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Immunohistochemistry(paraffin):
      Nestin (cat. # MAB353, Clone 401) staining of Mouse Pancreas, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25 µg/mL, using IHC-Select® detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.
      Optimal Staining of Nestin Monoclonal Antibody: Mouse Pancreas
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      IHC Select Paraffin Protocol
      MAB353 IH(P) Summary

      MSDS

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      Certificates of Analysis

      TitleLot Number
      Anti-Nestin, clone rat-401 - 21366192136619
      Anti-Nestin, clone rat-401 - 23701312370131
      Anti-Nestin, clone rat-401 - 24441412444141
      Anti-Nestin, clone rat-401 - LV1739283LV1739283
      Anti-Nestin, clone rat-401 - 19703291970329
      Anti-Nestin, clone rat-401 - 20199292019929
      Anti-Nestin, clone rat-401 - 20415692041569
      Anti-Nestin, clone rat-401 - 20901282090128
      Anti-Nestin, clone rat-401 - 22119432211943
      Anti-Nestin, clone rat-401 - 22967282296728
      Anti-Nestin, clone rat-401 - 23269842326984
      Anti-Nestin, clone rat-401 - 24890642489064
      Anti-Nestin, clone rat-401 - LV1575964LV1575964
      Anti-Nestin, clone rat-401 - LV1634942LV1634942
      Anti-Nestin, clone rat-401 - LV1714334LV1714334
      Anti-Nestin, clone rat-401 - LV1732492LV1732492
      Anti-Nestin, clone rat-401 - LV1770320LV1770320
      Anti-Nestin, clone rat-401 - LV1770361LV1770361
      Anti-Nestin, clone rat-401 - LV1797294LV1797294
      Anti-Nestin, clone rat-401 - NG1848434NG1848434
      Anti-Nestin, clone rat-401 -25006362500636

      References | 87 Available | See All References

      Reference overviewPub Med ID
      Plasticity of calcium signaling cascades in human embryonic stem cell-derived neural precursors.
      Forostyak, Oksana, et al.
      Stem Cells Dev., 22: 1506-21 (2013) 2013

      Show Abstract
      23294113
      Cell of origin determines tumor phenotype in an oncogenic Ras/p53 knockout transgenic model of high-grade glioma.
      Ghazi, Sabah O, et al.
      J. Neuropathol. Exp. Neurol., 71: 729-40 (2012) 2012

      Show Abstract
      22805776
      Ablation of vacuole protein sorting 18 (vps18) gene leads to neurodegeneration and impaired neuronal migration by disrupting multiple vesicle transport pathways to lysosomes.
      Chao Peng,Jian Ye,Shunfei Yan,Shanshan Kong,Ye Shen,Chenyu Li,Qinyu Li,Yufang Zheng,Kejing Deng,Tian Xu,Wufan Tao
      The Journal of biological chemistry 287 2012

      Show Abstract
      22854957
      Activation of specific interneurons improves V1 feature selectivity and visual perception.
      Seung-Hee Lee,Alex C Kwan,Siyu Zhang,Victoria Phoumthipphavong,John G Flannery,Sotiris C Masmanidis,Hiroki Taniguchi,Z Josh Huang,Feng Zhang,Edward S Boyden,Karl Deisseroth,Yang Dan
      Nature 488 2012

      Show Abstract
      22878719
      Btg1 is Required to Maintain the Pool of Stem and Progenitor Cells of the Dentate Gyrus and Subventricular Zone.
      Stefano Farioli-Vecchioli,Laura Micheli,Daniele Saraulli,Manuela Ceccarelli,Sara Cannas,Raffaella Scardigli,Luca Leonardi,Irene Cin,Marco Costanzi,Maria Teresa Ciotti,Pedro Moreira,Jean-Pierre Rouault,Vincenzo Cestari,Felice Tirone,Irene Cinà
      Frontiers in neuroscience 6 2012

      Show Abstract
      22969701
      The novel function of Oct3/4 in mouse tooth development.
      Eizo Nakagawa,Li Zhang,Eun-Jung Kim,Jeong-Oh Shin,Sung-Won Cho,Hayato Ohshima,Han-Sung Jung
      Histochemistry and cell biology 137 2012

      Show Abstract
      22159899
      A Role for the Cannabinoid 1 Receptor in Neuronal Differentiation of Adult Spinal Cord Progenitors in vitro is Revealed through Pharmacological Inhibition and Genetic Deletion.
      Alexandra Sideris,Tatiana Bekker,Wai Si Chan,Jose V Montoya-Gacharna,Thomas J J Blanck,Esperanza Recio-Pinto
      Frontiers in neuroscience 6 2012

      Show Abstract
      22291615
      Plasma membrane-associated glycohydrolases along differentiation of murine neural stem cells.
      Massimo Aureli,Angela Gritti,Rosaria Bassi,Nicoletta Loberto,Alessandra Ricca,Vanna Chigorno,Alessandro Prinetti,Sandro Sonnino
      Neurochemical research 37 2012

      Show Abstract
      22350518
      Nestin expression is lost in ventricular fibroblasts during postnatal development of the rat heart and re-expressed in scar myofibroblasts.
      Pauline C Béguin,Hugues Gosselin,Maya Mamarbachi,Angelino Calderone
      Journal of cellular physiology 227 2012

      Show Abstract
      21503881
      Mcl-1 regulates the survival of adult neural precursor cells.
      Craig D Malone,S M Mahmudul Hasan,R Brian Roome,Jieying Xiong,Michael Furlong,Joseph T Opferman,Jacqueline L Vanderluit
      Molecular and cellular neurosciences 49 2012

      Show Abstract
      22357134

      Data Sheet

      Title
      Anti-Nestin, clone rat-401 - Data Sheet