MAB3402 | Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

Western Blot Analysis:
Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Glial Fibrillary Acidic Protein (1:1000 dilution of this lot).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates protein Glial Fibrillary Acidic Protein (~51 kDa).
Western Blot Analysis:
Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF
MAB3402
40 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Rb, H, R, M, Po, Ch, BWB, ICC, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB3402
      Brand Family Chemicon®
      Synonyms GFAP
      Trade Name
      • Chemicon
      Description Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5
      Background Information Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
      References
      Product Information
      Format Purified
      Specificity The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
      Control
      • Mouse brain tissue, Astrocyte culture.
      Presentation Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
      Applications
      Application Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application Notes Immunocytochemistry:
      5 μg/mL of a previous lot was used.

      Immunohistochemistry:
      5 µg/mL

      Immunoblotting:
      Recognizes a 51kDa protein in reducing westerns of total brain lysates.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen species Purified glial filament (Debus, E., 1983).
      Clone GA5
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
      Isotype IgG1
      Species Reactivity Rabbit Human Rat Mouse Pig Chicken Bovine
      Species Reactivity Note Human, mouse, and rat. Expected to cross-react with porcine, chicken, bovine, and rabbit.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • FLJ45472
      • GFAP
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 432 amino acids; 49880 Da
      SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on Mouse brain lysate.

      Western Blot Analysis:
      1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
      DO NOT FREEZE.
      Packaging Information
      Material Size 40 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Glial Fibrillary Acidic Protein, clone GA5 2465212
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2118035 2118035
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2430439 2430439
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 1944055 1944055
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 1993774 1993774
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2019952 2019952
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2049225 2049225
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2191972 2191972
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2210339 2210339
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2301652 2301652
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - 2327472 2327472
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - LV1467441 LV1467441
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - LV1594099 LV1594099
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - LV1634987 LV1634987
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - LV1689941 LV1689941
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - LV1746173 LV1746173
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - NG1878855 NG1878855
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - NG1914008 NG1914008
      Anti-Glial Fibrillary Acidic Protein, clone GA5 -2510227 2510227

      References | 60 Available | See All References

      Reference overviewApplicationSpeciesPub Med ID
      Hypoxia-inducible factor 1 protects hypoxic astrocytes against glutamate toxicity.
      Yomna Badawi,Prabhu Ramamoorthy,Honglian Shi
      ASN neuro 4 2012

      Show Abstract
      22540931
      Genetic ablation of Nrf2/antioxidant response pathway in Alexander disease mice reduces hippocampal gliosis but does not impact survival.
      Tracy L Hagemann,Emily M Jobe,Albee Messing
      PloS one 7 2012

      Show Abstract
      22693571
      Toll-like receptors 2 and 3 agonists differentially affect oligodendrocyte survival, differentiation, and myelin membrane formation.
      Malika Bsibsi,Anita Nomden,Johannes M van Noort,Wia Baron
      Journal of neuroscience research 90 2012

      Show Abstract
      21971760
      An optimized protocol for the acute isolation of human microglia from autopsy brain samples.
      Marta Olah,Divya Raj,Nieske Brouwer,Alexander H De Haas,Bart J L Eggen,Wilfred F A Den Dunnen,Knut P H Biber,Hendrikus W G M Boddeke
      Glia 60 2012

      Show Abstract
      21989594
      Neuroprotective function for ramified microglia in hippocampal excitotoxicity.
      Jonathan Vinet,Hilmar Rj van Weering,Annette Heinrich,Roland E Kälin,Anja Wegner,Nieske Brouwer,Frank L Heppner,Nico van Rooijen,Hendrikus Wgm Boddeke,Knut Biber,Hilmar R J van Weering,Nico van Rooijen,Hendrikus W G M Boddeke
      Journal of neuroinflammation 9 2012

      Show Abstract
      22293457
      The expression of calcitonin receptor detected in malignant cells of the brain tumour glioblastoma multiforme and functional properties in the cell line A172.
      Peter J Wookey,Catriona A McLean,Peter Hwang,Sebastian G B Furness,Sandy Nguyen,Angela Kourakis,David L Hare,Jeffrey V Rosenfeld
      Histopathology 60 2012

      Show Abstract
      22335784
      Indirect immunofluorescence staining of cultured neural cells.
      Massimo Barbierato,Carla Argentini,Stephen D Skaper
      Methods in molecular biology (Clifton, N.J.) 846 2012

      Show Abstract
      22367816
      Overexpression of HGF attenuates the degeneration of Purkinje cells and Bergmann glia in a knockin mouse model of spinocerebellar ataxia type 7.
      Satsuki Noma,Wakana Ohya-Shimada,Masaaki Kanai,Keiji Ueda,Toshikazu Nakamura,Hiroshi Funakoshi
      Neuroscience research 73 2012

      Show Abstract
      22426494
      Effect of curcumin in a mouse model of Pelizaeus-Merzbacher disease.
      Li-Hua Yu,Toshifumi Morimura,Yurika Numata,Ryoko Yamamoto,Naoko Inoue,Barbara Antalfy,Yu-Ichi Goto,Kimiko Deguchi,Hitoshi Osaka,Ken Inoue
      Molecular genetics and metabolism 106 2012

      Show Abstract
      22436581
      Ezh2 expression in astrocytes induces their dedifferentiation toward neural stem cells.
      Sher, Falak, et al.
      Cell Reprogram, 13: 1-6 (2011) 2011

      Show Abstract
      20979531

      Data Sheet

      Title
      Anti-Glial Fibrillary Acidic Protein, clone GA5 - Data Sheet

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      Categories

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