MAB3068 | Anti-Connexin 43 Antibody, CT

Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CONNEXIN 43 (1:500 dilution of this lot).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Arrow indicates protein CONNEXIN 43 (~43 kDa).
Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF
MAB3068
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, Ca, Ch, H, MWB, ELISA, ICC, IF, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB3068
      Brand Family Chemicon®
      Synonyms gap junction protein, alpha-like Gap junction 43 kDa heart protein connexin 43 gap junction protein, alpha 1, 43kDa gap junction protein, alpha 1, 43kDa (connexin 43)
      Trade Name
      • Chemicon
      Description Anti-Connexin 43 Antibody, CT
      Background Information Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.
      References
      Product Information
      Format Purified
      Specificity Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse fibroblasts. Also effective for detection of Cx43 in human fibroblasts, rat brain, canine ventricular myocytes, and goldfish astrocytes. The molecular weight range (43-37 kDa) detected in Western blot of mouse 3T3 fibroblasts likely represents post-translational modifications of a 43 kDa primary translation product [Mol. Cell Biol. (1990) 10: 1754].
      Control
      • Rat brain lysate
      Presentation Purified mouse monoclonal IgG1 liquid in buffer containing aqueous buffered solution containing 50% glycerol, BSA and < 0.09% sodium azide
      Applications
      Application Detect Connexin 43 using this Anti-Connexin 43 Antibody, C-terminus validated for use in ELISA, IC, IF, IH, IP & WB.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      Application Notes Western blot:
      1:500 Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse fibroblasts.

      ELISA:
      A previous lot of this antibody was used in Enzyme-linked Immunosorbent Assay.

      Immunofluorescence:
      A previous lot of this antibody was used in Enzyme-linked Immunosorbent Assay.

      Immunohistochemistry:
      (2% paraformaldehyde fixation) of 12 μm cryosections of canine Purkinje fibers. 1:250-1:1000 dilution of a previous lot was used with incubation for 16 hours at 4ºC prior to fluorescence detection [Circulation Res. (1993) 72: 1124].

      Immunocytochemistry (acetone fixation):
      1:50-1:300 dilution of a previous lot was used for fluorescence detection (see Mol. Cell Biol (1990) 10: 1754). More sensitive methods of detection will permit increased antibody dilution.

      Immunoprecipitation:
      A previous lot of this antibody was used in immunoprecipitation.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen species A synthetic peptide corresponding to positions 252-270 of the native sequence from rat cardiac connexin 43 [NH2-GDLSPSKDCGSPK YAYFNGK-COOH]
      Epitope C-terminus
      Clone RN26
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse fibroblasts. Also effective for detection of Cx43 in human fibroblasts, rat brain, canine ventricular myocytes, and goldfish astrocytes. The molecular weight range (43-37 kDa) detected in Western blot of mouse 3T3 fibroblasts likely represents post-translational modifications of a 43 kDa primary translation product [Mol. Cell Biol. (1990) 10: 1754].
      Isotype IgG1
      Species Reactivity Rat Canine Chicken Human Mouse
      Species Reactivity Note Human, rat, canine, mouse, chick.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.
      Gene Symbol
      • Connexin-43
      • GJA1
      • CX43
      • GJAL
      • Cx43
      • ODD
      • DFNB38
      • ODDD
      • ODOD
      • SDTY3
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
      SIZE: 382 amino acids; 43008 Da
      SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
      TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea.
      DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
      SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on Mouse brain lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected CONNEXIN 43 on 10 μg of Mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Connexin 43, C-terminus 2483213
      Anti-Connexin 43, C-terminus - 2343133 2343133
      Anti-Connexin 43, C-terminus - 2392243 2392243
      Anti-Connexin 43, C-terminus - 1994667 1994667
      Anti-Connexin 43, C-terminus - 2090918 2090918
      Anti-Connexin 43, C-terminus - 2191934 2191934
      Anti-Connexin 43, C-terminus - 2287708 2287708
      Anti-Connexin 43, C-terminus - JBC1766826 JBC1766826
      Anti-Connexin 43, C-terminus - LV1459882 LV1459882
      Anti-Connexin 43, C-terminus - LV1519578 LV1519578
      Anti-Connexin 43, C-terminus - NG1719692 NG1719692
      Anti-Connexin 43, C-terminus - NG1843011 NG1843011

      References | 32 Available | See All References

      Reference overviewPub Med ID
      Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.
      Joanna Gemel,Tasha K Nelson,Janis M Burt,Eric C Beyer
      The Journal of membrane biology 245 2012

      Show Abstract
      22729648
      Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance.
      Megan L McCain,Thomas Desplantez,Nicholas A Geisse,Barbara Rothen-Rutishauser,Helene Oberer,Kevin Kit Parker,Andre G Kleber
      American journal of physiology. Heart and circulatory physiology 302 2012

      Show Abstract
      22081700
      Corneal surface reconstruction using adult mesenchymal stem cells in experimental limbal stem cell deficiency in rabbits.
      Helga Reinshagen,Claudia Auw-Haedrich,Ruediger V Sorg,Daniel Boehringer,Philipp Eberwein,Johannes Schwartzkopff,Rainer Sundmacher,Thomas Reinhard
      Acta ophthalmologica 89 2011

      Show Abstract
      20039850
      E-cadherin contributes to the bystander effect of TK/GCV suicide therapy and enhances its antitumoral activity in pancreatic cancer models.
      L Garcia-Rodríguez,D Abate-Daga,A Rojas,J R González,C Fillat
      Gene therapy 18 2011

      Show Abstract
      20720574
      Connexin-26 is a key factor mediating gemcitabine bystander effect.
      Garcia-Rodríguez L, Pérez-Torras S, Carrió M, Cascante A, García-Ribas I, Mazo A, Fillat C
      Mol Cancer Ther 10 505-17. 2011

      Show Abstract
      21388975
      Influence of v5/6-his tag on the properties of gap junction channels composed of connexin43, connexin40 or connexin45.
      Desplantez T, Halliday D, Dupont E, Severs NJ, Weingart R
      J Membr Biol 240 139-50 2011

      Show Abstract
      21424225
      Hippocampal seizures alter the expression of the pannexin and connexin transcriptome.
      Mylvaganam S, Zhang L, Wu C, Zhang ZJ, Samoilova M, Eubanks J, Carlen PL, Poulter MO
      J Neurochem 112 92-102. Epub 2009 Oct 13. 2010

      Show Abstract
      19840216
      Changes of connexin43 expression in non-pregnant porcine myometrium correlate with progesterone concentration during oestrous cycle.
      J Karasinski,J Galas,D Semik,A Fiertak,B Bilinska,W M Kilarski
      Reproduction in domestic animals = Zuchthygiene 45 2010

      Show Abstract
      20149142
      Activator of G protein signaling 8 (AGS8) is required for hypoxia-induced apoptosis of cardiomyocytes: role of G betagamma and connexin 43 (CX43).
      M Sato, Q Jiao, T Honda, R Kurotani, E Toyota, S Okumura, T Takeya, S Minamisawa, SM Lanier, Y Ishikawa
      The Journal of biological chemistry 284 31431-40 2009

      Show Abstract Full Text Article
      19723622
      ZO-1 is required for protein kinase C gamma-driven disassembly of connexin 43.
      Vladimir Akoyev,Dolores J Takemoto
      Cellular signalling 19 2007

      Show Abstract Full Text Article
      17210245

      Data Sheet

      Title
      Anti-Connexin 43, C-terminus - Data Sheet