MAB2137 | Anti-Homing Receptor Antibody, clone A020

MAB2137
100  μg
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Rb, B, Ca, H, M, Po, RWB, ELISA, FC, ICC, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB2137
      Brand Family Chemicon®
      Synonyms CD44s; HCAM; Pgp-1
      Trade Name
      • Chemicon
      Description Anti-Homing Receptor Antibody, clone A020
      References
      Product Information
      Format Purified
      Specificity Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).
      Control
      • Cells of epithelium and highly expressed by carcinomas
      Presentation Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.
      Applications
      Application Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.
      Key Applications
      • Western Blotting
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      Application Notes ELISA 1:2000

      Flow Cytometry :1:1000

      Immunofluorescence 1:200

      Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.

      Immunoprecipitation 1:200

      Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen species Purified human blood lymphocyte CD44
      Clone A020
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Rat
      Specificity Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).
      Isotype IgG2b
      Species Reactivity Rabbit Bovine Canine Human Mouse Pig Rat
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
      Gene Symbol
      • HCELL
      • CD44
      • LHR
      • HUTCH-I
      • CDW44
      • CDw44
      • ECMR-III
      • Epican
      • MGC10468
      • CD44R
      • PGP-1
      • MDU2
      • CSPG8
      • Pgp1
      • IN
      • MUTCH-I
      • MIC4
      • MDU3
      • MC56
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P16070 # Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
      SIZE: 742 amino acids; 81554 Da
      SUBUNIT: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
      PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. & N-glycosylated. & O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). & Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
      SIMILARITY: SwissProt: P16070 ## Contains 1 Link domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Qty/Pck 100 μg
      Material Size 100
      Material Package μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Millipore - COA - RAT ANTI-CD44S MONOCLONAL ANTIBODY - 2147028 2147028
      RAT ANTI-CD44S MONOCLONAL ANTIBODY - 2398866 2398866
      RAT ANTI-CD44S MONOCLONAL ANTIBODY - 2295633 2295633

      References

      Reference overviewApplicationSpeciesPub Med ID
      Peripheral facial nerve axotomy in mice causes sprouting of motor axons into perineuronal central white matter: time course and molecular characterization.
      Milan Makwana,Alexander Werner,Alejandro Acosta-Saltos,Roman Gonitel,Abirami Pararajasingham,Crystal Ruff,Prakasham Rumajogee,Dan Cuthill,Mathias Galiano,Marion Bohatschek,Adam S Wallace,Patrick N Anderson,Ulrike Mayer,Axel Behrens,Gennadij Raivich
      The Journal of comparative neurology 518 2010

      Show Abstract
      20034058
      Mature human thymocytes migrate on laminin-5 with activation of metalloproteinase-14 and cleavage of CD44.
      Vivinus-Nebot, Mylène, et al.
      J. Immunol., 172: 1397-406 (2004) 2004

      Show Abstract
      Immunoblotting (Western)Human14734715
      Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration
      Kajita, M, et al.
      J Cell Biol, 153:893-904 (2001) 2001

      ImmunocytochemistryHuman11381077
      Correlation of CD44S expression in renal clear cell carcinomas with subsequent tumor progression or recurrence.
      M Z Gilcrease, M Guzman-Paz, G Niehans, D Cherwitz, J B McCarthy, J Albores-Saavedra, M Z Gilcrease, M Guzman-Paz, G Niehans, D Cherwitz, J B McCarthy, J Albores-Saavedra
      Cancer 86 2320-6 1999

      Show Abstract
      10590373
      Hyaluronic acid-induced lymphocyte signal transduction and HA receptor (GP85/CD44)-cytoskeleton interaction.
      Bourguignon, L Y, et al.
      J. Immunol., 151: 6634-44 (1993) 1993

      Show Abstract
      7505012

      Brochure

      Title
      Human Stem Cell Systems

      Data Sheet

      Title
      Anti-Homing Receptor, clone A020 - Data Sheet