MAB1501R | Anti-Actin Antibody, near a.a. 50-70, clone C4

Immunocytochemistry Analysis:
Confocal IF analysis of Hela, NIH/3T3 using anti-Actin Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue).
Immunocytochemistry Analysis:
Confocal IF analysis of Hela, NIH/3T3 using anti-Actin Mouse mAB
MAB1501R
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AELISA, WB, IH(P), IHC, ICCMProtein G PurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB1501R
      Brand Family Chemicon®
      Synonyms actin, alpha 1, skeletal muscle SMA smooth muscle actin actin MAB1501X MAB1501 alpha skeletal muscle actin
      Trade Name
      • Chemicon
      Description Anti-Actin Antibody, near a.a. 50-70, clone C4
      Background Information Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).
      References
      Product Information
      Format Protein G Purified
      Specificity MAB1501R is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      Control
      • HeLa whole cell lysate.
      Presentation Protein G Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
      Applications
      Application This Anti-Actin Antibody, near a.a. 50-70, clone C4 is validated for use in ELISA, IC, IH, IH(P), WB for the detection of Actin.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunohistochemistry
      • Immunocytochemistry
      Application Notes Immunocytochemistry:
      10 μg/mL dilution from a previous lot was used (methanol fixed mouse 3T3 cells).

      Immunohistochemistry:
      10μg/mL dilution from a previous lot was used for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

      ELISA:
      A previous lot was shown to be strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      Western blot:
      1-20 µg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle (Otey, 1987).

      Immunohistochemistry:
      10µg/mL for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      Immunogen species Purified chicken gizzard actin (Lessard, 1988).
      Epitope a.a. 50-70
      Clone C4
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity MAB1501R is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      Isotype IgG1κ
      Species Reactivity All
      Species Reactivity Note To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
      Gene Symbol
      • ACTA1
      • NEM3
      • CFTD1
      • Alpha-actin-1
      • NEM2
      • CFTD
      • ACTA
      • CFTDM
      • NEM1
      • ASMA
      • MPFD
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
      SIZE: 377 amino acids; 42051 Da
      SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
      DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
      SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
      MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on HeLa lysate.

      Western Blot Analysis:
      1:1000 dilution of this lot detected actin on 10 μg of HeLa lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 6 months at 2-8°C from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Actin, Clone C4 - 2142171 2142171
      Anti-Actin, Clone C4 - 2387816 2387816
      Anti-Actin, Clone C4 - 2391073 2391073
      Anti-Actin, Clone C4 - 2459631 2459631
      Anti-Actin, Clone C4 - 1991258 1991258
      Anti-Actin, Clone C4 - 2020280 2020280
      Anti-Actin, Clone C4 - 2066001 2066001
      Anti-Actin, Clone C4 - 2188493 2188493
      Anti-Actin, Clone C4 - 2279536 2279536
      Anti-Actin, Clone C4 - 2489021 2489021
      Anti-Actin, Clone C4 - LV1611401 LV1611401
      Anti-Actin, Clone C4 - LV1688376 LV1688376
      Anti-Actin, Clone C4 - LV1728681 LV1728681
      Anti-Actin, Clone C4 - LV1746178 LV1746178
      Anti-Actin, Clone C4 - LV1746179 LV1746179
      Anti-Actin, Clone C4 - NG1853933 NG1853933
      Anti-Actin, Clone C4 - NG1867683 NG1867683
      Anti-Actin, near a.a. 50-70, clone C4 - LV1530193 LV1530193

      References | 68 Available | See All References

      Reference overviewPub Med ID
      Mutations in IRX5 impair craniofacial development and germ cell migration via SDF1.
      Carine Bonnard,Anna C Strobl,Mohammad Shboul,Hane Lee,Barry Merriman,Stanley F Nelson,Osama H Ababneh,Elif Uz,Tülay Güran,Hülya Kayserili,Hanan Hamamy,Bruno Reversade
      Nature genetics 44 2012

      Show Abstract
      22581230
      Headless Myo10 Is a Negative Regulator of Full-length Myo10 and Inhibits Axon Outgrowth in Cortical Neurons.
      Alexander N Raines,Sarbajeet Nagdas,Michael L Kerber,Richard E Cheney
      The Journal of biological chemistry 287 2012

      Show Abstract
      22661706
      Regulation of cyclin T1 expression and function by an alternative splice variant that skips exon 7 and contains a premature termination codon.
      Emiko Urano,Kosuke Miyauchi,Reiko Ichikawa,Yuko Futahashi,Jun Komano
      Gene 505 2012

      Show Abstract
      22692005
      Murine Lupus Susceptibility Locus Sle1c2 Mediates CD4+ T Cell Activation and Maps to Estrogen-Related Receptor γ.
      Daniel J Perry,Yiming Yin,Tiffany Telarico,Henry V Baker,Igor Dozmorov,Andras Perl,Laurence Morel
      Journal of immunology (Baltimore, Md. : 1950) 189 2012

      Show Abstract
      22711888
      Mechanisms and roles of muscarinic activation in guinea-pig adrenal medullary cells.
      Masumi Inoue,Keita Harada,Hidetada Matsuoka,Jun Nakamura,Akira Warashina
      American journal of physiology. Cell physiology 303 2012

      Show Abstract
      22744007
      The molecular mechanism of apoptosis upon caspase-8 activation: quantitative experimental validation of a mathematical model.
      Katsuya Kominami,Jun Nakabayashi,Takeharu Nagai,Yuki Tsujimura,Kumiko Chiba,Haruna Kimura,Atsushi Miyawaki,Tatsuya Sawasaki,Hideo Yokota,Noboru Manabe,Kazuhiro Sakamaki
      Biochimica et biophysica acta 1823 2012

      Show Abstract
      22801217
      Promoters Recognized by Forkhead Proteins Exist for Individual 21U-RNAs.
      Germano Cecere,Grace X Y Zheng,Andres R Mansisidor,Katherine E Klymko,Alla Grishok
      Molecular cell 47 2012

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      22819322
      Corneal goblet cells and their niche: implications for corneal stem cell deficiency.
      Ahdeah Pajoohesh-Ganji,Sonali Pal-Ghosh,Gauri Tadvalkar,Mary Ann Stepp
      Stem cells (Dayton, Ohio) 30 2012

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      22821715
      Autophagy: Resetting glutamine-dependent metabolism and oxygen consumption.
      Tsung-Chin Lin,Yun-Ru Chen,Elizabeth Kensicki,Angela Ying-Jian Li,Mei Kong,Yang Li,Robert P Mohney,Han-Ming Shen,Bangyan Stiles,Noboru Mizushima,Liang-In Lin,David K Ann
      Autophagy 8 2012

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      22906967
      Therapeutic reactivation of mutant p53 protein by quinazoline derivatives.
      Hamish S Sutherland,In Young Hwang,Elaine S Marshall,Brent S Lindsay,William A Denny,Catherine Gilchrist,Wayne R Joseph,Debra Greenhalgh,Emma Richardson,Philip Kestell,Angela Ding,Bruce C Baguley
      Investigational new drugs 30 2012

      Show Abstract
      21912889

      Data Sheet

      Title
      Anti-Actin, near a.a. 50-70, clone C4 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies