MAB1281 | Anti-Nuclei Antibody, clone 235-1

Mouse anti-Human Nuclei (Catalogue No. MAB1281) staining of human neural stem cells transplanted into rat brain. Photo courtesy of ReNeuron.
Mouse anti-Human Nuclei (Catalogue No. MAB1281) staining of human neural stem cells transplanted int
MAB1281
100  µL
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Human OnlyICC, IH(P), IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number MAB1281
      Brand Family Chemicon®
      Synonyms HuNu
      Trade Name
      • Chemicon
      Description Anti-Nuclei Antibody, clone 235-1
      Background Information Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.
      References
      Product Information
      Format Purified
      Specificity Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
      Control
      • All human cell types
      Presentation Hybridoma supernatant. Liquid in PBS. Contains no preservative.
      Applications
      Application Detect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application Notes Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500.

      Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended.
      http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm

      Identifies an 82 and 70 kD protein by immunoprecipitation.

      Optimal working dilutions and protocols must be determined by the end user.
      Biological Information
      Clone 235-1
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
      Isotype IgG1
      Species Reactivity Human Only
      Species Reactivity Note The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.
      Antibody Type Monoclonal Antibody
      Purification Method Protein A purfied
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Qty/Pck 100 μL
      Material Size 100
      Material Package µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2366521 2366521
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2453144 2453144
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2287154 2287154
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2127768 2127768
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 1969098 1969098
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2000372 2000372
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2055436 2055436
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2101964 2101964
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2199561 2199561
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2239593 2239593
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - JC1682922 JC1682922
      Millipore - COA - MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - LV1514200 LV1514200

      References | 69 Available | See All References

      Reference overviewApplicationPub Med ID
      Effect of Intermittent PTH(1-34) on Human Periodontal Ligament Cells Transplanted into Immunocompromised Mice.
      Michael Wolf,Stefan Lossd,Nuersailike Abuduwali,Rainer Meyer,Sied Kebir,Werner G,Andreas J
      Tissue engineering. Part A 18 2012

      Show Abstract
      22497226
      Cell-derived matrix coatings for polymeric scaffolds.
      Martin L Decaris,Bernard Y Binder,Matthew A Soicher,Archana Bhat,J Kent Leach
      Tissue engineering. Part A 18 2012

      Show Abstract
      22651377
      Neuroblast survival depends on mature vascular network formation after mouse stroke: role of endothelial and smooth muscle progenitor cell co-administration.
      Lina R Nih,Nicolas Deroide,Carole Leré-Déan,Dominique Lerouet,Mathieu Soustrat,Bernard I Levy,Jean-Sébastien Silvestre,Tatiana Merkulova-Rainon,Marc Pocard,Isabelle Margaill,Nathalie Kubis
      The European journal of neuroscience 35 2012

      Show Abstract
      22512253
      Activation of Multiple ERBB Family Receptors Mediates Glioblastoma Cancer Stem-like Cell Resistance to EGFR-Targeted Inhibition.
      Paul A Clark,Mari Iida,Daniel M Treisman,Haviryaji Kalluri,Sathyapriya Ezhilan,Michael Zorniak,Deric L Wheeler,John S Kuo
      Neoplasia (New York, N.Y.) 14 2012

      Show Abstract
      22745588
      Increasing doublecortin expression promotes migration of human embryonic stem cell-derived neurons.
      Radmila Filipovic,Saranya Santhosh Kumar,Chris Fiondella,Joseph Loturco
      Stem cells (Dayton, Ohio) 30 2012

      Show Abstract
      22753232
      Generation and hepatic differentiation of human iPS cells.
      Tetsuya Ishikawa,Keitaro Hagiwara,Takahiro Ochiya
      Methods in molecular biology (Clifton, N.J.) 826 2012

      Show Abstract
      22167643
      Significant clinical, neuropathological and behavioural recovery from acute spinal cord trauma by transplantation of a well-defined somatic stem cell from human umbilical cord blood.
      Jessica Schira,Marcia Gasis,Veronica Estrada,Marion Hendricks,Christine Schmitz,Thorsten Trapp,Fabian Kruse,Gesine Kögler,Peter Wernet,Hans-Peter Hartung,Hans Werner Müller
      Brain : a journal of neurology 135 2012

      Show Abstract
      21903726
      A novel in vivo model for the study of human breast cancer metastasis using primary breast tumor-initiating cells from patient biopsies.
      Carolyn G Marsden,Mary Jo Wright,Latonya Carrier,Krzysztof Moroz,Radhika Pochampally,Brian G Rowan
      BMC cancer 12 2012

      Show Abstract
      22233382
      Integration potential of mouse and human bone marrow-derived mesenchymal stem cells.
      Maria Kuzma-Kuzniarska,Aleksandra Rak-Raszewska,Simon Kenny,David Edgar,Bettina Wilm,Cristina Fuente Mora,Jamie A Davies,Patricia Murray
      Differentiation; research in biological diversity 83 2012

      Show Abstract
      22364880
      Recurrent genomic instability of chromosome 1q in neural derivatives of human embryonic stem cells.
      Christine Varela,Jérôme Alexandre Denis,Jérôme Polentes,Maxime Feyeux,Sophie Aubert,Benoite Champon,Geneviève Piétu,Marc Peschanski,Nathalie Lefort
      The Journal of clinical investigation 122 2012

      Show Abstract
      22269325

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation