IHCR1001-6 | Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60

IHCR1001-6
6 mL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, Av, Ch, Ft, H, M, Po, R, Pm, SalIH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number IHCR1001-6
      Brand Family Chemicon®
      Synonyms NeuN
      Trade Name
      • Chemicon
      Description Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60
      Background Information NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
      References
      Product Information
      Format Purified
      Specificity neuron-specific protein NeuN
      Presentation Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% ProClin 300 as preservative.
      Applications
      Application Anti-Neuron-Specific Nuclear Protein Antibody, prediluted, clone A60 detects level of Neuron-Specific Nuclear Protein & has been published & validated for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application Notes Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

      Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

      Incubation: 30 minutes with IHC Select® Detection Kits.

      NeuN, has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1001-6.
      Biological Information
      Immunogen species Purified cell nuclei from mouse brain.
      Clone A60
      Host Mouse
      Specificity neuron-specific protein NeuN
      Species Reactivity Vertebrates Avian Chicken Ferret Human Mouse Pig Rat Primate Salamander
      Antibody Type Monoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain at 2-8°C. Refer to vial for expiration dating.
      Packaging Information
      Material Size 6 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      CHEMICON IHC SELECT RESEARCH#210; - 2204904 2204904
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN - 2059278 2059278
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 1995236 1995236
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 2005949 2005949
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 2036277 2036277
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 2110562 2110562
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 2192694 2192694
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - NG1877942 NG1877942
      CHEMICON IHC SELECT RESEARCH 2470887
      CHEMICON IHC SELECT RESEARCH PREDILUTED MOUSE ANTI-NEURON SPECIFIC NUCLEAR PROTEIN MONOCLONAL ANTIBODY - 2426534 2426534

      Data Sheet

      Title
      Anti-Neuron-Specific Nuclear Protein, prediluted, clone A60 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies