HADP-61HK | Human Adiponectin RIA

HADP-61HK
125 tubes  
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      AdiponectinHRIARadioactive, 125I
      Description
      Catalogue Number HADP-61HK
      Description Human Adiponectin RIA
      Background Information Adiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
      References
      Product Information
      Detection method Radioactive 125I
      Specificity Human Adiponectin 100%
      C1q Human < 0.01%~
      Mouse Adiponectin 400%
      Performance
      • ED80 = 3.9 ± 0.9 ng/mL
        ED50 = 21.0 ± 5.7 ng/mL
        ED20 = 128.0 ± 48.6 ng/mL
      Precision, %
      • Inter-assay: 6.9 – 9.3 (serum sample concentration 1:500 dilution – 3 ng/mL, 6 ng/mL, 15 ng/mL)
      • Intra-assay: 1.8 – 6.2 (serum sample concentration 1:500 dilution – 3 ng/mL, 6 ng/mL, 15 ng/mL)
      Applications
      Application This radioimmunoassay (RIA) measures & quantifies Adiponectin for use in Metabolism & Endocrine research.
      Key Applications
      • Radioimmunoassay
      Application Notes Overnight incubation at room temperature.
      Sample volume <2 μL serum or plasma, or <100 μL tissue culture media
      Biological Information
      Specificity Human Adiponectin 100%
      C1q Human < 0.01%~
      Mouse Adiponectin 400%
      Species Reactivity Human
      Analytes Available
      • Adiponectin
      Entrez Gene Number
      Entrez Gene Summary This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
      Gene Symbol
      • ADIPOQ
      • APM-1
      • ACDC
      • adiponectin
      • apM1
      • ACRP30
      • GBP28
      • APM1
      • apM-1
      • AdipoQ
      Label 125I-Adiponectin
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
      SIZE: 244 amino acids; 26414 Da
      SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
      PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
      DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.
      SIMILARITY: Contains 1 C1q domain. & Contains 1 collagen-like domain.
      MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. & HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW- complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls.
      Accuracy
      • 116% (serum sample concentration 1:500 dilution–5 ng/mL standard)
      • 101% (serum sample concentration 1:500 dilution–100 ng/mL standard)
      • 111% (serum sample concentration 1:500 dilution–20 ng/mL standard)
      Physicochemical Information
      Sensitivity
      • 0.78 ng/mL
      Linearity of Dilution 85–119%
      Standard Curve Range
      • 0.78–200 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size 125 tubes
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      HUMAN ADIPONECTIN RIA KIT

      References | 33 Available | See All References

      Reference overviewPub Med ID
      Association of visceral and subcutaneous fat with glucose intolerance, insulin resistance, adipocytokines and inflammatory markers in Asian Indians (CURES-113).
      Indulekha K, Anjana RM, Surendar J, Mohan V
      Clin Biochem 44 281-7. Epub 2011 Jan 8. 2011

      Show Abstract
      21219897
      The Effect of Dietary Fish Oil in addition to Lifestyle Counselling on Lipid Oxidation and Body Composition in Slightly Overweight Teenage Boys.
      Maiken Højgaard Pedersen,Christian Mølgaard,Lars Ingvar Hellgren,Jeppe Matthiessen,Jens Juul Holst,Lotte Lauritzen
      Journal of nutrition and metabolism 2011 2011

      Show Abstract Full Text Article
      21773017
      Postprandial plasma adiponectin response is reduced in prepubertal premature pubarche girls.
      Larque E, Gil-Campos M, Villada I, Ramirez-Tortosa M, Canete R, Gil A
      Metabolism: clinical and experimental 2010

      Show Abstract
      20096425
      Relationships between contraction properties of knee extensor muscles and fasting IGF-1 and adipocytokines in physically active postmenopausal women.
      Jürimäe T, Pääsuke M, Kums T, Gapeyeva H, Ereline J, Saar M, Jürimäe J
      Clin Physiol Funct Imaging 2010

      Show Abstract
      20633033
      Serum total adiponectin is associated with impaired glucose tolerance in Asian Indian females but not in males.
      Menghua Luo,Reena Oza-Frank,K M Venkat Narayan,Kuppan Gokulakrishnan,Viswanathan Mohan
      Journal of diabetes science and technology 4 2010

      Show Abstract Full Text Article
      20513331
      Menopause impacts the relation of plasma adiponectin levels with the metabolic syndrome.
      Henneman P, Janssens AC, Zillikens MC, Frolich M, Frants RR, Oostra BA, van Duijn CM, van Dijk KW
      Journal of internal medicine 2009

      Show Abstract
      19912464
      Relationship Between Cerebrospinal Fluid Visfatin (PBEF/Nampt) Levels and Adiposity in Humans.
      Manfred Hallschmid, Harpal Randeva, Bee K Tan, Werner Kern, Hendrik Lehnert
      Diabetes 58 637-40 2009

      Show Abstract Full Text Article
      19095760
      Metabolic syndrome affects fatty acid composition of plasma lipids in obese prepubertal children.
      Mercedes Gil-Campos, Maria del Carmen Ramírez-Tortosa, Elvira Larqué, Javier Linde, Concepción M Aguilera, Ramón Cañete, Angel Gil
      Lipids 43 723-32 2008

      Show Abstract
      18592286
      The influence of ghrelin, adiponectin, and leptin on bone mineral density in healthy postmenopausal women.
      Jaak Jürimäe, Toivo Jürimäe, Aire Leppik, Tatjana Kums
      Journal of bone and mineral metabolism 26 618-23 2008

      Show Abstract
      18979162
      Dietary sugars stimulate fatty acid synthesis in adults.
      Elizabeth J Parks, Lauren E Skokan, Maureen T Timlin, Carlus S Dingfelder
      The Journal of nutrition 138 1039-46 2008

      Show Abstract Full Text Article
      18492831

      Data Sheet

      Title
      Bring your biomarkers to life.

      User Guides

      Title
      Quality Control Ranges: HUMAN ADIPONECTIN RIA KIT CATALOG # HADP-61HK