GF030 | Glial Derived Neurotrophic Factor Protein, Recombinant human

GF030
10 µg  
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      Overview

      Key Spec Table

      Species Source Purity
      HumanE.coliGreater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of human GDNF.
      Description
      Catalogue Number GF030
      Brand Family Chemicon®
      Synonyms GDNF
      Trade Name
      • Chemicon
      Description Glial Derived Neurotrophic Factor Protein, Recombinant human
      Overview Human Glial Derived Neurotrophic Factor (GDNF) is a 30.4 kDa protein that specifically promotes dopamine uptake and survival of midbrain neurons. GDNF is a disulphide linked homodimeric protein consisting of two 134 amino acid chains.
      References
      Product Information
      Presentation Lyophilized from 10 mM Sodium Citrate and 150 mM NaCl. Contains no preservative.
      Applications
      Application The human Glial Derived Neurotrophic Factor (GDNF) is a 30.4 kDa protein that specifically promotes dopamine uptake & survival of midbrain neurons.
      Biological Information
      Purity Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of human GDNF.
      Source E.coli
      Species Human
      Specific Activity Chemicon's human GDNF is fully biologically active when compared to standards as determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures. The ED(50) was found to be in the range of 5-10 ng/mL. <br />The ED(50) as determined by the proliferation of rat C6 cells is ≤ 0.1 ng/mL, corresponding to aspecific activity of ≥ 1 x 10<sup>7</sup> units/mg.
      Entrez Gene Number
      Entrez Gene Summary This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
      Gene Symbol
      • ATF
      • GDNF
      • HFB1-GDNF
      • ATF1
      • ATF2
      • hGDNF
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P39905 # Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
      SIZE: 211 amino acids; 23720 Da
      SUBUNIT: Homodimer; disulfide-linked.
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
      DISEASE: SwissProt: P39905 # Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. & Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
      SIMILARITY: SwissProt: P39905 ## Belongs to the TGF-beta family. GDNF subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. The lyophilized power can be reconstituted to 0.1-1.0 mg/mL. This solution can be diluted into water or other buffered solutions. Reconstituted GDNF should be stored in undiluted aliquots at -20°C for up to 6 months.
      Packaging Information
      Material Size 10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2115923 2115923
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2153001 2153001
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2387471 2387471
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2458997 2458997
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2002166 2002166
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2309030 2309030
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2325037 2325037
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - 2494613 2494613
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR -2512414 2512414
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR -2528764 2528764

      References

      Reference overviewPub Med ID
      Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires.
      Vadim S Polikov,Jau-Shyong Hong,William M Reichert
      Journal of neuroscience methods 190 2010

      Show Abstract Full Text Article
      20470825
      Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits.
      Nathaniel A Sowa,Bonnie Taylor-Blake,Mark J Zylka
      The Journal of neuroscience : the official journal of the Society for Neuroscience 30 2010

      Show Abstract Full Text Article
      20147550

      Data Sheet

      Title
      RECOMBINANT HUMAN GLIAL DERIVED NEUROTROPHIC FACTOR - Data Sheet

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