FTAV00003 | Fast Trap Adenovirus Purification and Concentration Kit

FTAV00003
3 purifications and concentrations  3 purifications and concentrations
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      Overview

      Description
      Catalogue Number FTAV00003
      Trade Name
      • Fast-Trap
      Description Fast Trap Adenovirus Purification and Concentration Kit
      Overview The Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification of ≥ 3 mL of adenovirus serotype 5, up to a capacity of approximately 1x1013 total adenovirus particles.

      The purification kit protocol calls for releasing the virus from the infected cells using multiple freeze-thaw cycles, then collecting the crude adenovirus. Most of the large cellular debris is removed by centrifugation, leaving the viable virus particles in the supernatant. The supernatant is further clarified by passing it through a Steriflip-HV 0.45 μm filter. After adding a binding buffer, the virus solution is passed over a treated filter that adsorbs the virus particles, allowing the smaller cellular debris and media to pass through the filter. Following a wash to remove any weakly bound proteins, the virus is eluted off the filter with an elution buffer. Finally, the virus can be concentrated and exchanged into the desired buffer using an Amicon® Ultra centrifugal filter unit.

      For laboratory use only; not for diagnostic or in vivo use.
      References
      Product Information
      Components 3 – Fast-Trap virus filter units
      3 – Steriflip-HV filter units
      1 – 10x Binding buffer, 20 mL
      1 – Equilibration buffer, 100 mL
      1 – Wash buffer, 200 mL
      1 – Elution buffer, 21 mL
      3 – Amicon Ultra-4 filter units, 50 kDa NMWL
      1 – Test tube stand
      1 – User Guide
      1 – Overview of Procedure card
      1 – Material Safety Data Sheet
      Applications
      Key Applications
      • Adenovirus Purification and Concentration
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • General Lab Use
      Storage and Shipping Information
      Storage Conditions The Fast-Trap Adenovirus Purification and Concentration Kit should be stored at room temperature.

      Warnings and Precautions:
      WARNING: This kit permits quick purification of adenovirus, an infectious agent which should be handled under BSL2 safety precautions. Wear hand, eye, face and body personal protective
      equipment (PPE) when using this kit. Millipore Corporation takes no responsibility for improper use of this kit.

      WARNING:All virus purification steps (except water bath incubation and centrifugation) should be conducted in a biosafety cabinet.
      For research use only. Not for use in diagnostic or human clinical procedures.
      Variation in the amount of adenovirus purified with this kit may be due in part to infection success and cell conditions.
      Packaging Information
      Material Size 3 purifications and concentrations
      Material Package 3 purifications and concentrations
      Transport Information
      Supplemental Information
      Specifications