FCABS259F | Milli-Mark™ Anti-Caspase 1-FITC Antibody

Flow Cytometry Analysis:
Representative lot data.
FC analysis of HL-60 cells stained with Anti-Caspase-1, FITC conjugated. Cells were stained with the antibody (green) or an isotype control (grey).
Flow Cytometry Analysis:
Representative lot data.
FC analysis of HL-60 cells stained wit
FCABS259F
100 tests  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCRbFITCPolyclonal Antibody
      Description
      Catalogue Number FCABS259F
      Synonyms interleukin 1-beta convertase
      CASP1 nirs variant 1 IL-1 beta-converting enzyme Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme caspase 1 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase) interleukin 1-B converting enzyme
      Trade Name
      • Milli-Mark
      Description Milli-Mark™ Anti-Caspase 1-FITC Antibody
      Background Information Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an ezyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.
      References
      Product Information
      Format FITC
      Specificity Antibody recognizes Human Caspase 1 antigen.
      Control
      • HL-60 cells
      Presentation Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

      Applications
      Application This Milli-Mark Anti-Caspase 1-FITC Antibody is validated for use in FC for the detection of Caspase 1.
      Key Applications
      • Flow Cytometry
      Biological Information
      Immunogen species KLH-conjugated, synthetic peptide corresponding to amino acid residues 129-149 of human Caspase 1.
      Host Rabbit
      Specificity Antibody recognizes Human Caspase 1 antigen.
      Isotype IgG
      Species Reactivity Human
      Species Reactivity Note Tested and passed on Human.
      Antibody Type Polyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summary This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
      Gene Symbol
      • ICE
      • CASP1
      • IL1BCE
      • P45
      • IL-1BC
      • IL1BC
      • p45
      • IL1B-convertase
      • CASP-1
      Purification Method Protein A Purified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

      SIZE: 404 amino acids; 45159 Da

      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.

      SUBCELLULAR LOCATION: Cytoplasm.

      TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

      PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

      SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by flow cytometry using HL-60 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
      Packaging Information
      Material Size 100 tests
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies