FAK100 | Actin Cytoskeleton / Focal Adhesion Staining Kit

Figure 1. Confocal fluorescence microscopy of focal adhesion and actin cytoskeleton in NIH-3T3 cells. (A) F-actin was detected using TRITC-conjugated phalloidin, (B) focal contacts were revealed using anti-Vinculin monoclonal antibody, (C) nuclear counterstaining revealed with DAPI, (D) triple staining with TRITC-conjugated phalloidin, anti-vinculin and DAPI.
Figure 1. Confocal fluorescence microscopy of focal adhesion and actin cytoskeleton in NIH-3T3 cell
FAK100
100 assays  
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      Overview

      Key Spec Table

      Species ReactivityKey Applications
      B, Rb, Po, HICC
      Description
      Catalogue Number FAK100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Actin Cytoskeleton / Focal Adhesion Staining Kit
      Overview The Actin Cytoskeleton and Focal Adhesion Staining Kit consists of three components (TRITC-conjugated phalloidin, anti-Vinculin and DAPI) for the immunofluorescent staining of actin filaments in the cytoskeleton, focal contacts as well as the nucleus of the cells.

      Reagents and materials supplied in the Actin Cytoskeleton and Focal Adhesion Staining Kit are sufficient for 100 tests (including controls).

      Introduction

      The actin cytoskeleton is a highly dynamic network composed of actin polymers and a large variety of associated proteins. The functions of the actin cytoskeleton is to mediate a variety of essential biological functions in all eukaryotic cells, including intra- and extra-cellular movement and structural support. To perform these functions, the organization of the actin cytoskeleton must be tightly regulated both temporally and spatially. Many proteins associated with the actin cytoskeleton are thus likely targets of signaling pathways controlling actin assembly. Actin cytoskeleton assembly is regulated at multiple levels, including the organization of actin monomers (G-protein) into actin polymers and the superorganization of actin polymers into a filamentous network (F-actin - the major constituent of microfilaments) [1]. This superorganization of actin polymers into a filamentous network is mediated by actin side-binding or cross-linking proteins [2, 3, 4]. Actin cytoskeleton is a dynamic structure that rapidly changes shape and organization in reponse to stimuli and cell cycle progression. Therefore, a disruption of its normal regulation may lead to cell transformations, hence cancer. Transformed cells have been shown to contain less F-actin than untransformed cells and exhibit atypical coordination of F-actin levels to the cell cycle [5]. Orientational distribution of actin filaments within a cell is an important determinant of cellular shape and motility. Therefore,

      Focal adhesion and adherens junctions are membrane-associated complexes that serve as nucleation sites for actin filaments and as cross-linkers between the cell exterior, plasma membrane and actin cytoskeleton [6]. The function of focal adhesions are structural, linking the ECM on the outside to the actin cytoskeleton on the inside. They are also sites of signal transduction, initiating of signaling pathways in response to adhesion. Focal adhesions consist of integrin-type receptors that are attached to the extracellular matrix and are intracellularly associated with protein complexes containing vinculin (universal focal adhesion marker), talin, a-actinin, paxillin, tensin, zyxin and focal adhesion kinase (FAK) [7,8].

      CHEMICON's Actin Cytoskeleton and Focal Adhesion Staining Kit (Catalog number FAK100) is a very sensitive immunocytochemical tool that contains fluorescent-labeled phalloidin (TRITC-conjugated phalloidin) to map the local orientation of actin filaments within cell and a mouse monoclonal antibody to Vinculin that is very specific for the staining of focal contacts in cells.
      References
      Product Information
      Components Vinculin Monoclonal antibody, purified clone 7F9 (Part No. 90227). One vial containing 100 μL at 1 mg/mL. DAPI (Part No. 90229). One vial containing 100 μL. TRITC-conjugated Phalloidin (Part No. 90228). One vial containing 15 μg.
      Applications
      Application The Actin Cytoskeleton & Focal Adhesion Staining Kit consists of TRITC-conjugated phalloidin, anti-Vinculin & DAPI for the immunofluorescent staining of actin filaments in the cytoskeleton as well as the nucleus of the cells.
      Key Applications
      • Immunocytochemistry
      Biological Information
      Host Mouse
      Species Reactivity Bovine Rabbit Pig Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size 100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References | 22 Available | See All References

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      PloS one 7 2012

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      Effect of substrate stiffness on pulmonary fibroblast activation by TGF-β.
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      Mitochondria-targeted peptide MTP-131 alleviates mitochondrial dysfunction and oxidative damage in human trabecular meshwork cells.
      Min Chen,Bingqian Liu,Qianying Gao,Yehong Zhuo,Jian Ge
      Investigative ophthalmology & visual science 52 2011

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      How to assess cytotoxicity of (iron oxide-based) nanoparticles: a technical note using cationic magnetoliposomes.
      Stefaan J H Soenen,Marcel De Cuyper
      Contrast media & molecular imaging 6 2011

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      21698773
      A novel shell-structure cell microcarrier (SSCM) for cell transplantation and bone regeneration medicine.
      Kai Su,Yihong Gong,Chunming Wang,Dong-An Wang
      Pharmaceutical research 28 2011

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      Balance of life and death in alveolar epithelial type II cells: proliferation, apoptosis, and the effects of cyclic stretch on wound healing.
      Lynn M Crosby,Charlean Luellen,Zhihong Zhang,Larry L Tague,Scott E Sinclair,Christopher M Waters
      American journal of physiology. Lung cellular and molecular physiology 301 2011

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      Lab on a chip 11 2011

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      Cell culture and motility study on a polymer surface with a nanometer-scaled stripe structure.
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      High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling.
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      In vitro cellular response and in vivo primary osteointegration of electrochemically modified titanium.
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      Brochure

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      User Guides

      Title
      Actin Cytoskeleton and Focal Adhesion Staining Kit

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents
      Life Science Research > Antibodies and Assays > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents