EZRMFGF21-26K | Rat / Mouse FGF-21 ELISA Kit

EZRMFGF21-26K
1  plates (96 wells)
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      FGF-21M, RELISAFluorescent, Colorimetric
      Description
      Catalogue Number EZRMFGF21-26K
      Description Rat / Mouse FGF-21 ELISA Kit
      Background Information Recent studies have demonstrated that fibroblast growth factor 21 (FGF-21), a liver derived protein, is able to increase glucose uptake, as well as lower plasma triglyceride levels in an insulin-independent manner. FGF-21 suppresses glucagon release, thereby increasing insulin sensitivity, and reduces the amount of insulin production by pancreatic β-cells. Unlike insulin, which works in a rapid, hormone-like manner, FGF-21 activity requires several hours, indicating changes in gene expression. No significant weight gain was observed when FGF-21 was injected into animals, and mice that had been genetically engineered to overexpress FGF-21 weighed less and had less adipose tissue than the normal controls.

      Although considerable progress has been made in the understanding of the pathobiology of type 2 diabetes, as well in the development of new compounds for the treatment of diabetic patients, this disease remains a major global health concern. Consequently, Millipore is pleased to offer the Rat/Mouse FGF-21 ELISA kit for the quantification of FGF-21 in rat and mouse serum and plasma samples. The insulin-independent activities of FGF-21, as well as the absence of the weight gain effects of insulin, thiazolidinediones and other anti-diabetic drugs, distinguish it from current insulin-sensitizing drugs, making it a promising therapeutic.
      References
      Product Information
      Detection method Fluorescent Colorimetric
      Specificity Cross-reactivity to Human FGF-21: 39%

      No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.
      Precision (%)
      • Inter-assay: < 8 %
      • Intra-assay: < 10%
      Applications
      Application This Rat / Mouse FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.
      Key Applications
      • ELISA
      Application Notes This is a 3-hour assay requiring 20 μL (in duplicate) of rat sample or 10µl (in duplicate) of mouse sample.
      Biological Information
      Specificity Cross-reactivity to Human FGF-21: 39%

      No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.
      Species Reactivity Mouse Rat
      Analytes Available
      • FGF-21
      Accuracy
      • Spike Recovery: 85 – 102 %
      Physicochemical Information
      Linearity of Dilution Please contact Technical Service for this information
      Standard Curve Range
      • 50 – 12000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Qty/Pck 96-well strip plate
      Material Size 1
      Material Package plates (96 wells)
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Rat/Mouse Fibroblast Growth Factor-21 (FGF-21)

      References

      Reference overviewPub Med ID
      Induction of ketosis in rats fed low-carbohydrate, high fat diets depends on the relative abundance of dietary fat and protein.
      Bielohuby, Maximilian, et al.
      American journal of physiology. Endocrinology and metabolism, (2010) 2010

      Show Abstract
      20943751

      User Guides

      Title
      Quality Control Ranges: RAT/MOUSE FGF-21 ELISA